Invitrogen

Huntingtin Monoclonal Antibody (HDC8A4)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-82100

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rabbit

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HDC8A4

Immunogen

Recombinant protein corresponding to amino acids 2703-2911 of huntingtin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2280122

Product Specific Information

Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol.

Mouse anti Huntingtin antibody, clone HDC8A4 reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein.

Target Information

Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HD protein; HD protein homolog; Huntingtin; Huntington disease gene homolog; Huntington disease protein; huntington disease protein homolog; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4

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Gene Aliases: AI256365; C430023I11Rik; HD; Hdh; HTT; IT15

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UniProt ID: (Human) P42858

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Entrez Gene ID: (Human) 3064, (Mouse) 15194, (Rabbit) 100351801

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Function(s)
p53 binding receptor binding protein binding profilin binding transcription factor binding dynactin binding identical protein binding ion channel binding dynein intermediate chain binding beta-tubulin binding diazepam binding
Process(es)
establishment of mitotic spindle orientation retrograde vesicle-mediated transport, Golgi to ER apoptotic process Golgi organization central nervous system development positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity regulation of protein phosphatase type 2A activity vocal learning positive regulation of cilium assembly vesicle transport along microtubule animal organ development mRNA transport negative regulation of cysteine-type endopeptidase activity negative regulation of extrinsic apoptotic signaling pathway urea cycle citrulline metabolic process protein import into nucleus mitochondrial transport ER to Golgi vesicle-mediated transport mitochondrion organization endoplasmic reticulum organization dopamine receptor signaling pathway spermatogenesis gastrulation brain development cell aging learning or memory learning grooming behavior locomotory behavior axo-dendritic transport associative learning determination of adult lifespan visual learning anatomical structure morphogenesis embryo development anterior/posterior pattern specification endosomal transport lactate biosynthetic process from pyruvate quinolinate biosynthetic process striatum development olfactory lobe development neural plate formation neurogenesis peptide hormone secretion insulin secretion social behavior hormone metabolic process negative regulation of apoptotic process negative regulation of neuron apoptotic process regulation of mitochondrial membrane permeability regulation of synaptic plasticity paraxial mesoderm formation neuron development neuron apoptotic process response to calcium ion regulation of mitochondrial membrane potential L-glutamate import iron ion homeostasis negative regulation of neuron death
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