Invitrogen

IBSP Polyclonal Antibody

1 Published Figure

View all (6) Bone SialoProtein antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

IBSP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IBSP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

IBSP Antibody (PA5-50633) in IHC (P)

Immunohistochemical analysis of IBSP in paraffin embedded Human colon cancer tissue using IBSP Polyclonal Antibody (Product # PA5-50633) at a 1:30 dilution.

IBSP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IBSP Antibody in Immunohistochemistry (IHC)

Product Image Gallery

IBSP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of IBSP in paraffin embedded Human colon cancer tissue using IBSP Polyclonal Antibody (Product # PA5-50633) at a 1:30 dilution.

IBSP Antibody in Immunohistochemistry (IHC) — Fig. 1 Bone-metastatic ER + breast cancer cells secrete miR-19a and IBSP. a EVs were prepared from the conditioned medium of bone-metastatic cell line MCF7BoM2 and the parental cell line MCF7, followed by extraction of RNA. They were then subjected to miRNA profiling by GeneAtlas miRNA Array. Using the criteria of FDR < 0.05 and Fold change > 1.3, 156 miRNAs were selected for further study. These miRNAs were then examined whether they are secreted in the blood of ER + patients using the cohort data set (GSE41922) of the circulating microRNA profiles between 23 ER + breast cancer (BC) patients and 22 healthy donors (Normal). Three miRNAs (miR-19a. miR-133b, miR-576-5p), were found to be significantly upregulated (FDR < 0.05 and logFC > 0.5) in the blood of breast cancer patients. BoM, bone metastatic. b Kaplan-Meier analysis of miR-19a was performed between breast cancer patients with low miR-19a and high miR-19a expression using GSE22220. c RNA from bone-met(metastatic) lesions ( n = 6), primary tumors ( n = 6), lung and brain lesions ( n = 6) of ER + breast cancer patients, and normal bones ( n = 4) was extracted and miR-19a expression was examined by Taqman-PCR. A two-sided student's t -test was performed (Primary vs Bone-met, p = 0.0047; Other-met vs Bone-met, p = 0.0249; Normal Bone vs Bone-met, p = 0.0059). Data are presented as mean values +- SEM. d The expression of miR-19a in parental cell lines (MCF7, T47D, and MDA-MB-231) and bone-tropic cell lines (MCF7BoM2, T47DBo

Product Details

PA5-50633

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:100

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region derived from internal residues of human integrin-binding sialoprotein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2636086

Product Specific Information

The antibody detects endogenous levels of total IBSP protein.

Target Information

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer.

Nature communications

Wu K,Feng J,Lyu F,Xing F,Sharma S,Liu Y,Wu SY,Zhao D,Tyagi A,Deshpande RP,Pei X,Ruiz MG,Takahashi H,Tsuzuki S,Kimura T,Mo YY,Shiozawa Y,Singh R,Watabe K

PA5-50633 was used in Immunohistochemistry (Paraffin), Western Blot to identify liquid biopsy biomarkers for patients with high risk of bone metastasis.

Tue Aug 31 00:00:00 UTC 2021

Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer.

Nature communications

Wu K,Feng J,Lyu F,Xing F,Sharma S,Liu Y,Wu SY,Zhao D,Tyagi A,Deshpande RP,Pei X,Ruiz MG,Takahashi H,Tsuzuki S,Kimura T,Mo YY,Shiozawa Y,Singh R,Watabe K

PA5-50633 was used in Immunohistochemistry (Paraffin), Western Blot to identify liquid biopsy biomarkers for patients with high risk of bone metastasis.

Tue Aug 31 00:00:00 UTC 2021

Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer.

Nature communications

Wu K,Feng J,Lyu F,Xing F,Sharma S,Liu Y,Wu SY,Zhao D,Tyagi A,Deshpande RP,Pei X,Ruiz MG,Takahashi H,Tsuzuki S,Kimura T,Mo YY,Shiozawa Y,Singh R,Watabe K

PA5-50633 was used in Immunohistochemistry (Paraffin), Western Blot to identify liquid biopsy biomarkers for patients with high risk of bone metastasis.

Tue Aug 31 00:00:00 UTC 2021

Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer.

Nature communications

Wu K,Feng J,Lyu F,Xing F,Sharma S,Liu Y,Wu SY,Zhao D,Tyagi A,Deshpande RP,Pei X,Ruiz MG,Takahashi H,Tsuzuki S,Kimura T,Mo YY,Shiozawa Y,Singh R,Watabe K

PA5-50633 was used in Immunohistochemistry (Paraffin), Western Blot to identify liquid biopsy biomarkers for patients with high risk of bone metastasis.

Tue Aug 31 00:00:00 UTC 2021

Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer.

Nature communications

Wu K,Feng J,Lyu F,Xing F,Sharma S,Liu Y,Wu SY,Zhao D,Tyagi A,Deshpande RP,Pei X,Ruiz MG,Takahashi H,Tsuzuki S,Kimura T,Mo YY,Shiozawa Y,Singh R,Watabe K

PA5-50633 was used in Immunohistochemistry (Paraffin), Western Blot to identify liquid biopsy biomarkers for patients with high risk of bone metastasis.

Tue Aug 31 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Bone sialoprotein 2; Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein

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Gene Aliases: BNSP; BSP; BSP-II; IBSP; SP-II

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UniProt ID: (Human) P21815

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Entrez Gene ID: (Human) 3381

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molecular_function

osteoblast differentiation cell adhesion extracellular matrix organization bone mineralization cellular response to growth factor stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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