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Invitrogen
ICSA Polyclonal Antibody
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ICSA Antibody (PA5-144412) in WB
Western blot analysis of ICSA in recombinant protein using a dilution of 1:2,000. Samples were incubated with ICSA polyclonal antibody (Product # PA5-144412). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 40.3 kDa. Observed band size: 45 kDa.
Product Details
PA5-144412
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Shigella flexneri ICSA protein (311-547aa)
Conjugate
Form
Liquid
Concentration
1.745 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091654
Target Information
An immunogenic protein essential for bacterial spreading, it elicits polar deposition of filamentous actin (actin-based motility) (PubMed:2542950, PubMed:8702989). Inside the host (human) cell mediates nucleation and polymerization of actin molecules on the bacterial surface, which provides the propulsive force for intracellular movement and intercellular dissemination of the bacterium (PubMed:2644195, PubMed:8468279, PubMed:2542950). During invasion of mammalian cells, triggers autophagy by binding to host ATG5. Interaction with IcsB leads to escape from the host autophagy defense system (PubMed:15576571). Binds ATP and displays weak ATPase activity (PubMed:8468279). Mediates polar adhesion to host cells and their invasion. The bile salt deoxycholate activates adhesion and invasion of host cells, possibly by altering IcsA's conformation. The adhesion and intercellular dissemination phenotypes can be separated (PubMed:24721572).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: virG
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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