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Invitrogen
IDH2 Monoclonal Antibody (6B13)
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FIGURE: 1 / 9
IDH2 Antibody (MA5-49159) in ICC/IF
Immunocytochemistry analysis of IDH2 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in IDH2 Monoclonal antibody (Product # MA5-49159) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Product Details
MA5-49159
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
6B13
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IDH2 (413-447aa KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074817
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Immunogen sequence identical to the related mouse and rat sequences.
Positive Control - WB: human Hela whole cell, human Sw620 whole cell, human MCF-7 whole cell, human HEPG2 whole cell, human Jurkat whole cell, rat heart tissue, rat liver tissue, rat PC-12 whole cell, mouse heart tissue, mouse liver tissue, mouse NIH/3T3 whole cell. IHC: human testicular cancer tissue, human rectal cancer tissue, human melanoma tissue, human ovarian cancer tissue, rat skeletal muscle tissue. ICC/IF: A431 cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NADas the electron acceptor and the other NADP. Five isocitrate dehydrogenases have been reported: three NAD-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ICD-M; IDH; IDP; isocitrate dehydrogenase 2 (NADP+), mitochondrial; Isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP+-specific isocitrate dehydrogenase; Oxalosuccinate decarboxylase
Gene Aliases: D2HGA2; E430004F23; ICD-M; IDH; Idh-2; IDH2; IDHM; IDP; IDPM; mNADP-IDH
UniProt ID: (Human) P48735, (Mouse) P54071, (Rat) P56574
Entrez Gene ID: (Human) 3418, (Mouse) 269951, (Rat) 361596
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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