Proteintech

IDH3A Polyclonal Antibody

IDH3A Antibody in Immunocytochemistry (ICC/IF)

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IDH3A Antibody (15909-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 15909-1-AP (IDH3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IDH3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IDH3A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15909-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IDH3A Fusion Protein Ag8662 (1-366 aa encoded by BC021967)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAGPAWISK VSRLLGAFHN PKQVTRGFTG GVQTVTLIPG DGIGPEISAA VMKIFDAAKA PIQWEERNVT AIQGPGGKWM IPSEAKESMD KNKMGLKGPL KTPIAAGHPS MNLLLRKTFD LYANVRPCVS IEGYKTPYTD VNIVTIRENT EGEYSGIEHV IVDGVVQSIK LITEGASKRI AEFAFEYARN NHRSNVTAVH KANIMRMSDG LFLQKCREVA ESCKDIKFNE MYLDTVCLNM VQDPSQFDVL VMPNLYGDIL SDLCAGLIGG LGVTPSGNIG ANGVAIFESV HGTAPDIAGK DMANPTALLL SAVMMLRHMG LFDHAARIEA ACFATIKDGK SLTKDLGGNA KCSDFTEEIC RRVKDLD (1-366 aa encoded by BC021967)

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of IDH3α Enhanced the Efficacy of Chemoimmunotherapy by Regulating Acidic Tumor Microenvironments.

Cancers

Zhang L,Song Y,Dai X,Xu W,Li M,Zhu Y

Thu Mar 16 00:00:00 UTC 2023

Inhibition of IDH3α Enhanced the Efficacy of Chemoimmunotherapy by Regulating Acidic Tumor Microenvironments.

Cancers

Zhang L,Song Y,Dai X,Xu W,Li M,Zhu Y

Thu Mar 16 00:00:00 UTC 2023

Inhibition of IDH3α Enhanced the Efficacy of Chemoimmunotherapy by Regulating Acidic Tumor Microenvironments.

Cancers

Zhang L,Song Y,Dai X,Xu W,Li M,Zhu Y

Thu Mar 16 00:00:00 UTC 2023

Inhibition of IDH3α Enhanced the Efficacy of Chemoimmunotherapy by Regulating Acidic Tumor Microenvironments.

Cancers

Zhang L,Song Y,Dai X,Xu W,Li M,Zhu Y

Thu Mar 16 00:00:00 UTC 2023

Mitochondrial protein hyperacetylation underpins heart failure with preserved ejection fraction in mice.

Journal of molecular and cellular cardiology

Liu X,Zhang Y,Deng Y,Yang L,Ou W,Xie M,Ding L,Jiang C,Yu H,Li Q,Li T

Fri Apr 01 00:00:00 UTC 2022

Mitochondrial protein hyperacetylation underpins heart failure with preserved ejection fraction in mice.

Journal of molecular and cellular cardiology

Liu X,Zhang Y,Deng Y,Yang L,Ou W,Xie M,Ding L,Jiang C,Yu H,Li Q,Li T

Fri Apr 01 00:00:00 UTC 2022

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

The EMBO journal

Xu Y,Shi T,Cui X,Yan L,Wang Q,Xu X,Zhao Q,Xu X,Tang QQ,Tang H,Pan D

Wed Dec 15 00:00:00 UTC 2021

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Nature communications

Wang XH,Xu S,Zhou XY,Zhao R,Lin Y,Cao J,Zang WD,Tao H,Xu W,Li MQ,Zhao SM,Jin LP,Zhao JY

Tue Jun 08 00:00:00 UTC 2021

Proteomic comparison of different synaptosome preparation procedures.

Amino acids

Gulyássy P,Puska G,Györffy BA,Todorov-Völgyi K,Juhász G,Drahos L,Kékesi KA

Tue Dec 01 00:00:00 UTC 2020

Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.

The Journal of investigative dermatology

Gu Z,Zhang H,Guo X,Cao Y

Sat Aug 01 00:00:00 UTC 2020

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.

Cell reports

Holderith N,Heredi J,Kis V,Nusser Z

Tue Jul 28 00:00:00 UTC 2020

A glycolytic shift in Schwann cells supports injured axons.

Nature neuroscience

Babetto E,Wong KM,Beirowski B

Thu Oct 01 00:00:00 UTC 2020

Mutant huntingtin does not cross the mitochondrial outer membrane.

Human molecular genetics

Hamilton J,Brustovetsky T,Khanna R,Brustovetsky N

Sat Oct 10 00:00:00 UTC 2020

Complement component 1q subcomponent binding protein in the brain of the rat.

Scientific reports

Barna J,Dimén D,Puska G,Kovács D,Csikós V,Oláh S,Udvari EB,Pál G,Dobolyi Á

Thu Mar 14 00:00:00 UTC 2019

Mouse Idh3a mutations cause retinal degeneration and reduced mitochondrial function.

Disease models & mechanisms

Findlay AS,Carter RN,Starbuck B,McKie L,Nováková K,Budd PS,Keighren MA,Marsh JA,Cross SH,Simon MM,Potter PK,Morton NM,Jackson IJ

Tue Dec 18 00:00:00 UTC 2018

Mouse Idh3a mutations cause retinal degeneration and reduced mitochondrial function.

Disease models & mechanisms

Findlay AS,Carter RN,Starbuck B,McKie L,Nováková K,Budd PS,Keighren MA,Marsh JA,Cross SH,Simon MM,Potter PK,Morton NM,Jackson IJ

Tue Dec 18 00:00:00 UTC 2018

Ifi27 is indispensable for mitochondrial function and browning in adipocytes.

Biochemical and biophysical research communications

Jin W,Jin W,Pan D

Mon Jun 18 00:00:00 UTC 2018

Ifi27 is indispensable for mitochondrial function and browning in adipocytes.

Biochemical and biophysical research communications

Jin W,Jin W,Pan D

Mon Jun 18 00:00:00 UTC 2018

Ifi27 is indispensable for mitochondrial function and browning in adipocytes.

Biochemical and biophysical research communications

Jin W,Jin W,Pan D

Mon Jun 18 00:00:00 UTC 2018

Ifi27 is indispensable for mitochondrial function and browning in adipocytes.

Biochemical and biophysical research communications

Jin W,Jin W,Pan D

Mon Jun 18 00:00:00 UTC 2018

Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Hu Z,Du M,Lai W,Liang Y,Liu Q,Mo Y,Bei J,Li S,Yang Y,Xu J,Cui L

Thu Jul 05 00:00:00 UTC 2018

Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Hu Z,Du M,Lai W,Liang Y,Liu Q,Mo Y,Bei J,Li S,Yang Y,Xu J,Cui L

Thu Jul 05 00:00:00 UTC 2018

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

Tue Dec 26 00:00:00 UTC 2017

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

Tue Dec 26 00:00:00 UTC 2017

Synaptic mitochondria: a brain mitochondria cluster with a specific proteome.

Journal of proteomics

Völgyi K,Gulyássy P,Háden K,Kis V,Badics K,Kékesi KA,Simor A,Györffy B,Tóth EA,Lubec G,Juhász G,Dobolyi A

Wed Apr 29 00:00:00 UTC 2015

Synaptic mitochondria: a brain mitochondria cluster with a specific proteome.

Journal of proteomics

Völgyi K,Gulyássy P,Háden K,Kis V,Badics K,Kékesi KA,Simor A,Györffy B,Tóth EA,Lubec G,Juhász G,Dobolyi A

Wed Apr 29 00:00:00 UTC 2015

Synaptic mitochondria: a brain mitochondria cluster with a specific proteome.

Journal of proteomics

Völgyi K,Gulyássy P,Háden K,Kis V,Badics K,Kékesi KA,Simor A,Györffy B,Tóth EA,Lubec G,Juhász G,Dobolyi A

Wed Apr 29 00:00:00 UTC 2015

Synaptic mitochondria: a brain mitochondria cluster with a specific proteome.

Journal of proteomics

Völgyi K,Gulyássy P,Háden K,Kis V,Badics K,Kékesi KA,Simor A,Györffy B,Tóth EA,Lubec G,Juhász G,Dobolyi A

Wed Apr 29 00:00:00 UTC 2015

Does the Rewarmed Heart Restore the Myocardial Proteome to That of the Pre-Cooled State?--A Proteomic Analysis of Surgical Samples.

Circulation journal : official journal of the Japanese Circulation Society

Oda T,Yamaguchi A,Shimizu K,Nikai T,Matsumoto K

Wed Sep 07 00:00:00 UTC 2016

Does the Rewarmed Heart Restore the Myocardial Proteome to That of the Pre-Cooled State?--A Proteomic Analysis of Surgical Samples.

Circulation journal : official journal of the Japanese Circulation Society

Oda T,Yamaguchi A,Shimizu K,Nikai T,Matsumoto K

Wed Sep 07 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase 3 (NAD+) alpha; Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH

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Gene Aliases: 1110003P10Rik; 1500012E04Rik; AA407078; AI316514; IDH3A

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UniProt ID: (Human) P50213, (Mouse) Q9D6R2, (Rat) Q99NA5

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Entrez Gene ID: (Human) 3419, (Mouse) 67834, (Rat) 114096

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Function(s)

magnesium ion binding isocitrate dehydrogenase (NAD+) activity NAD binding oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor metal ion binding

Process(es)

carbohydrate metabolic process tricarboxylic acid cycle isocitrate metabolic process 2-oxoglutarate metabolic process NADH metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IDH3A Polyclonal Antibody

IDH3A Antibody (15909-1-AP) in ICC/IF

Product Details

15909-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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