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IFI35 Antibody (CF503357) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFI35 (RC200929, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-IFI35. Positive lysates LY401696 (100 µg) and LC401696 (20 µg) can be purchased separately from OriGene.
Product Details
CF503357
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI2B9
Immunogen
Full length human recombinant protein of human IFI35 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Interferon-Induced Protein 35 (IFI35), encoded by the IFI35 gene, is located on chromosome 17 and is recognized for its role in immune response modulation, especially under viral stress conditions. Structurally, IFI35 is part of a complex regulatory network that influences non-canonical NF-kB signaling. This process involves the proteasomal processing of p105 into the DNA-binding transcription factor p50, which forms a heterodimer with RELB (RELB/p50) to activate cell chemotaxis autonomously. The gene's dynamic expression under viral stress has been linked to various immune regulatory networks and is crucial for immune cell recruitment and maintenance, particularly M2-like tumor-associated macrophages (TAMs) within the tumor microenvironment. IFI35 upregulation is significant in conditions of neuroinflammation and multiple sclerosis, especially in patients treated with interferons (IFN), indicating its potential as a biomolecular marker. Additionally, IFI35's role in triple-negative breast cancer highlights its impact on antitumor immunity, where its ablation enhances the infiltration of effector CD8+ T cells and sensitizes tumors to anti-PD-1 immunotherapy. This positions IFI35 as a key player in both immune response and cancer treatment strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ifi-35; IFP 35; Interferon-induced 35 kDa protein; interferon-induced protein 35
Gene Aliases: IFI35; IFP35
UniProt ID: (Human) P80217
Entrez Gene ID: (Human) 3430
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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