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Invitrogen
IFIT3 Polyclonal Antibody
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IFIT3 Antibody (PA5-89571) in WB
Western blot analysis of IFIT3 in extracts of various cell lines. Samples were incubated with IFIT3 Polyclonal antibody (Product # PA5-89571) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-89571
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse IFIT3 (NP_0346311)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805660
Product Specific Information
Positive Samples: HT-29, COS-7, Mouse liver; Cellular Location: Cytoplasm, Mitochondrion
Immunogen sequence: YAWIYYHMGR LSEAQAYVDK VRQVCQKFAN PYSMECPELE CEEGWTRLKC GRNERAKMCF EKALEEKPKD PECSSGMAIA MFRLEEKPEK QFSVDALKQA M
Target Information
IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CIG49; GARG-49; Glucocorticoid-attenuated response gene 49 protein; IFI-60K; IFIT-3; IFIT-4; Interferon-induced 60 kDa protein; Interferon-induced protein with tetratricopeptide repeats 3; Interferon-induced protein with tetratricopeptide repeats 4; IRG2; ISG-60; P60; Retinoic acid-induced gene G protein; RP11-149I23.4
Gene Aliases: CIG-49; cig41; GARG-49; Garg49; Ifi49; IFI60; IFIT3; IFIT4; IRG2; Isg49; ISG60; P49; P60; RIG-G
UniProt ID: (Human) O14879, (Mouse) Q64345
Entrez Gene ID: (Human) 3437, (Mouse) 15959, (Rat) 309526
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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