Proteintech

IFITM2/3 Monoclonal Antibody (2E8D12)

IFITM2/3 Antibody in Immunocytochemistry (ICC/IF)

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IFITM2/3 Antibody (66081-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IFITM2/3 antibody (Product # 66081-1-Ig) (Clone: 2E8D12) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

IFITM2/3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IFITM2/3 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

66081-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 6 publications 6 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E8D12

Immunogen

IFITM2/3 Fusion Protein Ag17863 (1-133 aa encoded by BC070243)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.85 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes both IFITM2 and IFITM3.

Immunogen sequence: MNHTVQTFF SPVNSGQPPN YEMLKEEHEV AVLGAPHNPA PPTSTVIHIR SETSVPDHVV WSLFNTLFMN PCCLGFIAFA YSVKSRDRKM VGDVTGAQAY ASTAKCLNIW ALILGILMTI LLIVIPVLIF QAYG (1-133 aa encoded by BC070243)

Target Information

IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Arenavirus Entry and Replication by the Cell-Intrinsic Restriction Factor ZMPSTE24 Is Enhanced by IFITM Antiviral Activity.

Frontiers in microbiology

Stott-Marshall RJ,Foster TL

Sun May 12 00:00:00 UTC 2024

Escape of HIV-1 envelope glycoprotein from the restriction of infection by IFITM3.

Journal of virology

Drouin A,Migraine J,Durand MA,Moreau A,Burlaud-Gaillard J,Beretta M,Roingeard P,Bouvin-Pley M,Braibant M

Mon Mar 01 00:00:00 UTC 2021

Syncytia formation by SARS-CoV-2-infected cells.

The EMBO journal

Buchrieser J,Dufloo J,Hubert M,Monel B,Planas D,Rajah MM,Planchais C,Porrot F,Guivel-Benhassine F,Van der Werf S,Casartelli N,Mouquet H,Bruel T,Schwartz O

Tue Dec 01 00:00:00 UTC 2020

Syncytia formation by SARS-CoV-2-infected cells.

The EMBO journal

Buchrieser J,Dufloo J,Hubert M,Monel B,Planas D,Rajah MM,Planchais C,Porrot F,Guivel-Benhassine F,Van der Werf S,Casartelli N,Mouquet H,Bruel T,Schwartz O

Tue Dec 01 00:00:00 UTC 2020

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

mBio

Ahi YS,Yimer D,Shi G,Majdoul S,Rahman K,Rein A,Compton AA

Tue Jan 21 00:00:00 UTC 2020

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

mBio

Ahi YS,Yimer D,Shi G,Majdoul S,Rahman K,Rein A,Compton AA

Tue Jan 21 00:00:00 UTC 2020

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Science (New York, N.Y.)

Buchrieser J,Degrelle SA,Couderc T,Nevers Q,Disson O,Manet C,Donahue DA,Porrot F,Hillion KH,Perthame E,Arroyo MV,Souquere S,Ruigrok K,Dupressoir A,Heidmann T,Montagutelli X,Fournier T,Lecuit M,Schwartz O

Fri Jul 12 00:00:00 UTC 2019

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Science (New York, N.Y.)

Fri Jul 12 00:00:00 UTC 2019

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.

International journal of molecular sciences

Winkler M,Wrensch F,Bosch P,Knoth M,Schindler M,Gärtner S,Pöhlmann S

Thu Aug 08 00:00:00 UTC 2019

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.

International journal of molecular sciences

Winkler M,Wrensch F,Bosch P,Knoth M,Schindler M,Gärtner S,Pöhlmann S

Thu Aug 08 00:00:00 UTC 2019

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Proceedings of the National Academy of Sciences of the United States of America

Shi G,Ozog S,Torbett BE,Compton AA

Tue Oct 23 00:00:00 UTC 2018

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Proceedings of the National Academy of Sciences of the United States of America

Shi G,Ozog S,Torbett BE,Compton AA

Tue Oct 23 00:00:00 UTC 2018

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Proceedings of the National Academy of Sciences of the United States of America

Shi G,Ozog S,Torbett BE,Compton AA

Tue Oct 23 00:00:00 UTC 2018

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Proceedings of the National Academy of Sciences of the United States of America

Shi G,Ozog S,Torbett BE,Compton AA

Tue Oct 23 00:00:00 UTC 2018

Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.

Frontiers in immunology