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Invitrogen
IFITM3 Polyclonal Antibody
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FIGURE: 1 / 6
IFITM3 Antibody (PA5-75524) in ICC/IF
Immunofluorescence analysis of IFITM3 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IFITM3 Polyclonal Antibody (Product # PA5-75524) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
Product Details
PA5-75524
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 9-54 of Human IFITM3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2719252
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
IFITM3 (interferon induced transmembrane protein 3), also known as 1-8U or IP15, is a multi-pass membrane protein that belongs to the IFITM (interferon inducible transmembrane) family of proteins. IFITM proteins are induced by type I and type II interferons and contain multiple interferon (IFN)-stimulated response elements (ISREs) in their promoter regions. IFITM proteins play important roles in many cellular processes and their expression requires the presence of the chromatin remodeling SWI/SNF-like BAF complexes. Cellular processes involving IFITM proteins include cellular anti-proliferative activities and homotypic cell adhesion functions of interferons. In addition, IFITM genes are often upregulated in various cancer cells, suggesting a possible role in carcinogenesis. Localizing to the membrane, IFITM3 is a 133 amino acid protein that is induced by IFN-alpha and IFN-gamma. IFITM3 expression can be regulated by TEF-1, Brg-1 and Sp1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dispanin subfamily A member 2b; DSPA2b; Fragilis; Fragilis 3; Fragilis protein; ifitm-like protein 1; Interferon-induced transmembrane protein 3; Interferon-inducible protein 1-8U; Interferon-inducible protein 15; interferon-inducible protein variant 10; Mil-1; Mouse ifitm-like protein 1
Gene Aliases: 1-8U; 1110004C05Rik; Cd225; Cdw217; DSPA2b; Fgls; IFITM3; IP15; mil-1; rat8
UniProt ID: (Human) Q01628, (Rat) P26376, (Mouse) Q9CQW9
Entrez Gene ID: (Human) 10410, (Rat) 361673, (Mouse) 66141
receptor-mediated endocytosis
heart development
negative regulation of cell proliferation
response to virus
negative regulation of viral transcription
response to interferon-gamma
response to interferon-alpha
response to interferon-beta
negative regulation of viral genome replication
negative regulation of viral entry into host cell
defense response to virus
cardiac muscle cell differentiation
type I interferon signaling pathway
immune system process
response to biotic stimulus
innate immune response
immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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