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          • Primary Antibodies ›
          • IFN gamma Antibodies

          Invitrogen

          IFN gamma Monoclonal Antibody (4S.B3), Biotin, eBioscience™

          80 Published Figures
          71 References
          View all (211) IFN gamma antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite IFN gamma Monoclonal Antibody (4S.B3), Biotin, eBioscience™

          • Published Figures (80)
          IFN gamma Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          IFN gamma Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 80

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          IFN gamma Antibody (13-7319-81) in Flow

          International journal of molecular sciences 2020 - The polarizations of monocyte and GBM progression in a co-cultured model under hypoxic conditions. ( A ) THP-1 monocytes were co-cultured with U87-green fluorescent protein (GFP) or U251-GFP under hypoxic conditions for 48 h. The co-cultured cells were plotted on a side scatter versus FITC. The THP-1 monocytes (GFP-negative cells) were analyzed to assess the levels of cluster of differentiation (CD) 206, ar... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in Flow Cytometry (Flow)
          IFN gamma Antibody in ELISA (ELISA)

          Product Details

          13-7319-81

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          -
          View 68 publications 68 publications

          ELISA (ELISA)

          0.5-2 µg/mL
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4S.B3

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • eFluor 450
          • eFluor 506
          • eFluor 660
          • FITC
          • PE 
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_466940

          Product Specific Information

          Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

          Applications Reported: The 4S.B3 antibody has been reported for use in ELISPOT, and ELISA.

          Applications Tested: The 4S.B3 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human interferon gamma (IFN gamma) in combination with the purified NIB42 (14-7318) or MD-1 (14-7317) antibodies for capture and recombinant human IFN gamma (29-8319) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g

          View more View less

          Gene Aliases: IFG; IFI; IFNG

          View more View less

          UniProt ID: (Human) P01579

          View more View less

          Entrez Gene ID: (Human) 3458

          View more View less

          Function(s)
          cytokine activity interferon-gamma receptor binding
          Process(es)
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter neutrophil apoptotic process regulation of the force of heart contraction adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response apoptotic process movement of cell or subcellular component humoral immune response cell cycle arrest cell surface receptor signaling pathway positive regulation of cell proliferation response to virus positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration antigen processing and presentation neutrophil chemotaxis negative regulation of epithelial cell differentiation endoplasmic reticulum unfolded protein response negative regulation of myelination positive regulation of synaptic transmission, cholinergic negative regulation of interleukin-17 production positive regulation of interleukin-12 production positive regulation of interleukin-23 production positive regulation of tumor necrosis factor production positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response positive regulation of peptidyl-serine phosphorylation of STAT protein positive regulation of smooth muscle cell apoptotic process positive regulation of T cell proliferation response to drug positive regulation of tyrosine phosphorylation of Stat1 protein defense response to bacterium defense response to protozoan negative regulation of growth of symbiont in host positive regulation of chemokine biosynthetic process positive regulation of interleukin-12 biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process positive regulation of neuron differentiation positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgG isotypes negative regulation of smooth muscle cell proliferation positive regulation of interleukin-1 beta secretion regulation of insulin secretion T cell receptor signaling pathway sensory perception of mechanical stimulus positive regulation of membrane protein ectodomain proteolysis defense response to virus positive regulation of killing of cells of other organism interferon-gamma-mediated signaling pathway regulation of interferon-gamma-mediated signaling pathway positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity positive regulation of fructose 1,6-bisphosphate metabolic process positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity cellular response to lipopolysaccharide cellular response to interleukin-18 positive regulation of establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway regulation of neuronal action potential positive regulation of exosomal secretion positive regulation of tumor necrosis factor (ligand) superfamily member 11 production regulation of hepatocyte proliferation
          It has to be done as per old AB suggested Products section.
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