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Invitrogen
IFRD1 Polyclonal Antibody
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FIGURE: 1 / 3
IFRD1 Antibody (PA5-100753) in ICC/IF
Immunocytochemistry analysis of IFRD1 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with IFRD1 polyclonal antibody (Product # PA5-100753) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100753
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IFRD1(Accession O00458), corresponding to amino acid residues G32-Y82.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850256
Product Specific Information
Antibody detects endogenous levels of total IFRD1.
Target Information
Tis7, also known as IFRD1, is a 442 amino acid containing membrane associated, non-nuclear intracellular protein belonging to the IFRD family. A regulatory protein expressed in a variety of tissues, Tis7 is known to play an important role in the regulation of gene activity in the proliferative and/or differentiative pathways induced by NGF. Reports suggest that Tis7 may be an autocrine factor that attenuates or amplifies the initial ligand induced signal and might also augment the adaptive response by stimulating the production of differentiated enterocytes. Tis7 acts as a negative regulator of transcriptional activity and represses expression of genes involved in myogenesis, muscle maintenance, and regeneration in a histone deacetylase dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12-O-tetradecanoylphorbol-13-acetate-induced sequence 7; Interferon-related developmental regulator 1; IRPR; Nerve growth factor-inducible protein PC4; pheochromocytoma cell-4; TIS7 protein; TPA induced sequence 7; TPA-induced sequence 7
Gene Aliases: Ifnl; IFRD1; PC4; TIS7
UniProt ID: (Human) O00458, (Mouse) P19182, (Rat) P20695
Entrez Gene ID: (Human) 3475, (Mouse) 15982, (Rat) 29596
myoblast fate determination
striated muscle tissue development
negative regulation of axon extension
skeletal muscle tissue regeneration
negative regulation of collateral sprouting
multicellular organism development
cell differentiation
muscle cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
neuroblast proliferation
adult somatic muscle development
glial cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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