Proteintech

IFT57 Polyclonal Antibody

IFT57 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

IFT57 Antibody (11083-1-AP) in ICC/IF

Immunofluorescent analysis of MDCK cells using 11083-1-AP (IFT57 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IFT57 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IFT57 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11083-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 10 publications 10 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Chicken, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IFT57 Fusion Protein Ag1566 (1-429 aa encoded by BC011899)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTAALAVVT TSGLEDGVPR SRGEGTGEVV LERGPGAAYH MFVVMEDLVE KLKLLRYEEE FLRKSNLKAP SRHYFALPTN PGEQFYMFCT LAAWLINKAG RPFEQPQEYD DPNATISNIL SELRSFGRTA DFPPSKLKSG YGEHVCYVLD CFAEEALKYI GFTWKRPIYP VEELEEESVA EDDAELTLNK VDEEFVEEET DNEENFIDLN VLKAQTYHLD MNETAKQEDI LESTTDAAEW SLEVERVLPQ LKVTIRTDNK DWRIHVDQMH QHRSGIESAL KETKGFLDKL HNEITRTLEK ISSREKYINN QLENLVQEYR AAQAQLSEAK ERYQQGNGGV TERTRLLSEV MEELEKVKQE MEEKGSSMTD GAPLVKIKQS LTKLKQETVE MDIRIGIVEH TLLQSKLKEK SNMTRNMHAT VIPEPATGFY (1-429 aa encoded by BC011899)

Target Information

HIPPI is required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import.

Molecular biology of the cell

Kobayashi T,Ishida Y,Hirano T,Katoh Y,Nakayama K

Fri Jan 01 00:00:00 UTC 2021

Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import.

Molecular biology of the cell

Kobayashi T,Ishida Y,Hirano T,Katoh Y,Nakayama K

Fri Jan 01 00:00:00 UTC 2021

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

The EMBO journal

Duan S,Li H,Zhang Y,Yang S,Chen Y,Qiu B,Huang C,Wang J,Li J,Zhu X,Yan X

Fri Jan 15 00:00:00 UTC 2021

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

The EMBO journal

Duan S,Li H,Zhang Y,Yang S,Chen Y,Qiu B,Huang C,Wang J,Li J,Zhu X,Yan X

Fri Jan 15 00:00:00 UTC 2021

Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.

Human molecular genetics

Dupont MA,Humbert C,Huber C,Siour Q,Guerrera IC,Jung V,Christensen A,Pouliet A,Garfa-Traoré M,Nitschké P,Injeyan M,Millar K,Chitayat D,Shannon P,Girisha KM,Shukla A,Mechler C,Lorentzen E,Benmerah A,Cormier-Daire V,Jeanpierre C,Saunier S,Delous M

Thu Aug 15 00:00:00 UTC 2019

Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.

Human molecular genetics

Thu Aug 15 00:00:00 UTC 2019

FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.

Proceedings of the National Academy of Sciences of the United States of America

Honda A,Kita T,Seshadri SV,Misaki K,Ahmed Z,Ladbury JE,Richardson GP,Yonemura S,Ladher RK

Tue Aug 14 00:00:00 UTC 2018

FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.

Proceedings of the National Academy of Sciences of the United States of America

Honda A,Kita T,Seshadri SV,Misaki K,Ahmed Z,Ladbury JE,Richardson GP,Yonemura S,Ladher RK

Tue Aug 14 00:00:00 UTC 2018

FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.

Proceedings of the National Academy of Sciences of the United States of America

Honda A,Kita T,Seshadri SV,Misaki K,Ahmed Z,Ladbury JE,Richardson GP,Yonemura S,Ladher RK

Tue Aug 14 00:00:00 UTC 2018

FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.

Proceedings of the National Academy of Sciences of the United States of America

Honda A,Kita T,Seshadri SV,Misaki K,Ahmed Z,Ladbury JE,Richardson GP,Yonemura S,Ladher RK

Tue Aug 14 00:00:00 UTC 2018

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.

eLife

Vuolo L,Stevenson NL,Heesom KJ,Stephens DJ

Tue Oct 16 00:00:00 UTC 2018

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.

eLife

Vuolo L,Stevenson NL,Heesom KJ,Stephens DJ

Tue Oct 16 00:00:00 UTC 2018

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.

eLife

Vuolo L,Stevenson NL,Heesom KJ,Stephens DJ

Tue Oct 16 00:00:00 UTC 2018

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.

eLife

Vuolo L,Stevenson NL,Heesom KJ,Stephens DJ

Tue Oct 16 00:00:00 UTC 2018

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Developmental cell

Kanie T,Abbott KL,Mooney NA,Plowey ED,Demeter J,Jackson PK

Mon Jul 10 00:00:00 UTC 2017

In Vitro Modeling Using Ciliopathy-Patient-Derived Cells Reveals Distinct Cilia Dysfunctions Caused by CEP290 Mutations.

Cell reports

Shimada H,Lu Q,Insinna-Kettenhofen C,Nagashima K,English MA,Semler EM,Mahgerefteh J,Cideciyan AV,Li T,Brooks BP,Gunay-Aygun M,Jacobson SG,Cogliati T,Westlake CJ,Swaroop A

Tue Jul 11 00:00:00 UTC 2017

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Nature communications

Taulet N,Vitre B,Anguille C,Douanier A,Rocancourt M,Taschner M,Lorentzen E,Echard A,Delaval B

Mon Dec 04 00:00:00 UTC 2017

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Nature communications

Taulet N,Vitre B,Anguille C,Douanier A,Rocancourt M,Taschner M,Lorentzen E,Echard A,Delaval B

Mon Dec 04 00:00:00 UTC 2017

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

PLoS genetics

Hsu Y,Garrison JE,Kim G,Schmitz AR,Searby CC,Zhang Q,Datta P,Nishimura DY,Seo S,Sheffield VC

Sun Oct 01 00:00:00 UTC 2017

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

PLoS genetics

Hsu Y,Garrison JE,Kim G,Schmitz AR,Searby CC,Zhang Q,Datta P,Nishimura DY,Seo S,Sheffield VC

Sun Oct 01 00:00:00 UTC 2017

Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex.

The Journal of biological chemistry

Katoh Y,Terada M,Nishijima Y,Takei R,Nozaki S,Hamada H,Nakayama K

Fri May 20 00:00:00 UTC 2016

Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex.

The Journal of biological chemistry

Katoh Y,Terada M,Nishijima Y,Takei R,Nozaki S,Hamada H,Nakayama K

Fri May 20 00:00:00 UTC 2016

Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex.

The Journal of biological chemistry

Katoh Y,Terada M,Nishijima Y,Takei R,Nozaki S,Hamada H,Nakayama K

Fri May 20 00:00:00 UTC 2016

Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex.

The Journal of biological chemistry

Katoh Y,Terada M,Nishijima Y,Takei R,Nozaki S,Hamada H,Nakayama K

Fri May 20 00:00:00 UTC 2016

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Nature cell biology

Raman M,Sergeev M,Garnaas M,Lydeard JR,Huttlin EL,Goessling W,Shah JV,Harper JW

Thu Oct 01 00:00:00 UTC 2015

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Nature cell biology

Raman M,Sergeev M,Garnaas M,Lydeard JR,Huttlin EL,Goessling W,Shah JV,Harper JW

Thu Oct 01 00:00:00 UTC 2015

Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.

Cell cycle (Georgetown, Tex.)

Siller SS,Burke MC,Li FQ,Takemaru K

Tue Aug 30 00:00:00 UTC 2016

Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.

Cell cycle (Georgetown, Tex.)

Siller SS,Burke MC,Li FQ,Takemaru K

Tue Aug 30 00:00:00 UTC 2016

DYNC2LI1 mutations broaden the clinical spectrum of dynein-2 defects.

Scientific reports

Kessler K,Wunderlich I,Uebe S,Falk NS,Gießl A,Brandstätter JH,Popp B,Klinger P,Ekici AB,Sticht H,Dörr HG,Reis A,Roepman R,Seemanová E,Thiel CT

Wed Jul 01 00:00:00 UTC 2015

DYNC2LI1 mutations broaden the clinical spectrum of dynein-2 defects.

Scientific reports

Kessler K,Wunderlich I,Uebe S,Falk NS,Gießl A,Brandstätter JH,Popp B,Klinger P,Ekici AB,Sticht H,Dörr HG,Reis A,Roepman R,Seemanová E,Thiel CT

Wed Jul 01 00:00:00 UTC 2015

Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.

PloS one

Xu Y,Cao J,Huang S,Feng D,Zhang W,Zhu X,Yan X

Mon Apr 18 00:00:00 UTC 2016

Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.

PloS one

Xu Y,Cao J,Huang S,Feng D,Zhang W,Zhu X,Yan X

Mon Apr 18 00:00:00 UTC 2016

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.

Developmental cell

Liew GM,Ye F,Nager AR,Murphy JP,Lee JS,Aguiar M,Breslow DK,Gygi SP,Nachury MV

Mon Nov 10 00:00:00 UTC 2014

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.

Developmental cell

Liew GM,Ye F,Nager AR,Murphy JP,Lee JS,Aguiar M,Breslow DK,Gygi SP,Nachury MV

Mon Nov 10 00:00:00 UTC 2014

Primary cilia signaling mediates intraocular pressure sensation.

Proceedings of the National Academy of Sciences of the United States of America

Luo N,Conwell MD,Chen X,Kettenhofen CI,Westlake CJ,Cantor LB,Wells CD,Weinreb RN,Corson TW,Spandau DF,Joos KM,Iomini C,Obukhov AG,Sun Y

Tue Sep 02 00:00:00 UTC 2014

Primary cilia signaling mediates intraocular pressure sensation.

Proceedings of the National Academy of Sciences of the United States of America

Luo N,Conwell MD,Chen X,Kettenhofen CI,Westlake CJ,Cantor LB,Wells CD,Weinreb RN,Corson TW,Spandau DF,Joos KM,Iomini C,Obukhov AG,Sun Y

Tue Sep 02 00:00:00 UTC 2014

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Nature cell biology

Zhao H,Zhu L,Zhu Y,Cao J,Li S,Huang Q,Xu T,Huang X,Yan X,Zhu X

Sun Dec 01 00:00:00 UTC 2013

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Nature cell biology

Zhao H,Zhu L,Zhu Y,Cao J,Li S,Huang Q,Xu T,Huang X,Yan X,Zhu X

Sun Dec 01 00:00:00 UTC 2013

BBS proteins interact genetically with the IFT pathway to influence SHH-related phenotypes.

Human molecular genetics

Zhang Q,Seo S,Bugge K,Stone EM,Sheffield VC

Tue May 01 00:00:00 UTC 2012

BBS proteins interact genetically with the IFT pathway to influence SHH-related phenotypes.

Human molecular genetics

Zhang Q,Seo S,Bugge K,Stone EM,Sheffield VC

Tue May 01 00:00:00 UTC 2012

BBS proteins interact genetically with the IFT pathway to influence SHH-related phenotypes.

Human molecular genetics

Zhang Q,Seo S,Bugge K,Stone EM,Sheffield VC

Tue May 01 00:00:00 UTC 2012

BBS proteins interact genetically with the IFT pathway to influence SHH-related phenotypes.

Human molecular genetics

Zhang Q,Seo S,Bugge K,Stone EM,Sheffield VC

Tue May 01 00:00:00 UTC 2012

A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.

PLoS genetics

Seo S,Zhang Q,Bugge K,Breslow DK,Searby CC,Nachury MV,Sheffield VC

Tue Nov 01 00:00:00 UTC 2011

A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.

PLoS genetics

Seo S,Zhang Q,Bugge K,Breslow DK,Searby CC,Nachury MV,Sheffield VC

Tue Nov 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Dermal papilla-derived protein 8; estrogen-related receptor beta like 1; Estrogen-related receptor beta-like protein 1; FLJ10147; HIP1 protein interactor; HIP1-interacting protein; huntingtin interacting protein-1 interacting protein; huntingtin-interacting protein-1 protein interactor; intraflagellar transport 57 homolog; Intraflagellar transport protein 57 homolog; MHS4R2; Vestrogen-related receptor beta like 1

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Gene Aliases: 4833420A15Rik; DERP8; ESRRBL1; HIPPI; IFT57; MHS4R2

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UniProt ID: (Human) Q9NWB7, (Mouse) Q8BXG3

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Entrez Gene ID: (Human) 55081, (Dog) 478553, (Mouse) 73916, (Rat) 303968

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Function(s)

DNA binding protein binding

Process(es)

neural tube closure heart looping transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process smoothened signaling pathway intraciliary transport cilium assembly regulation of apoptotic process negative regulation of epithelial cell proliferation left/right pattern formation cilium morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFT57 Polyclonal Antibody

IFT57 Antibody (11083-1-AP) in ICC/IF

Product Details

11083-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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