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IGF1 Antibody (500-P157GBT-25UG) in WB
Western Blot: To detect mIGF-I by Western Blot analysis IGF1 Polyclonal Antibody, Biotin (Product # 500-P157GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P157GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IGF-I
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929538
Product Specific Information
AA Sequence of recombinant protein: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine IGF-I. Anti-Murine IGF-I-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mIGF-I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IGF-I (500-P157G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIGF-I.
Western Blot: To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P157GBT-1MG will be provided as 2 x 500 µg
Target Information
IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1A; IGFIa; Insulin like growth factor; Insulin-like growth factor 1; Insulin-like growth factor I; M-IGF-1; OTTHUMP00000195084; R-IGF-1; Somatomedin; Somatomedin C
Gene Aliases: C730016P09Rik; Igf-1; Igf-I; Igf1
UniProt ID: (Mouse) P05017
Entrez Gene ID: (Mouse) 16000
cell activation
regulation of protein phosphorylation
blood vessel remodeling
regulation of translation
nervous system development
memory
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
response to heat
glial cell differentiation
regulation of gene expression
positive regulation of cardiac muscle hypertrophy
phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration
muscle hypertrophy
myotube cell development
positive regulation of smooth muscle cell migration
positive regulation of cerebellar granule cell precursor proliferation
water homeostasis
proteoglycan biosynthetic process
positive regulation of cell growth
lung development
positive regulation of cell migration
mammary gland development
exocrine pancreas development
regulation of establishment or maintenance of cell polarity
regulation of intracellular steroid hormone receptor signaling pathway
positive regulation of protein import into nucleus, translocation
negative regulation of smooth muscle cell apoptotic process
multicellular organism growth
bone mineralization involved in bone maturation
positive regulation of activated T cell proliferation
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of DNA binding
positive regulation of MAPK cascade
protein kinase B signaling
positive regulation of insulin-like growth factor receptor signaling pathway
myoblast differentiation
positive regulation of fat cell differentiation
positive regulation of osteoblast differentiation
positive regulation of glycogen biosynthetic process
positive regulation of DNA replication
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
positive regulation of Ras protein signal transduction
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
lung alveolus development
cell development
positive regulation of smooth muscle cell proliferation
branching morphogenesis of an epithelial tube
inner ear development
chondroitin sulfate proteoglycan biosynthetic process
positive regulation of epithelial cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
detection of mechanical stimulus involved in sensory perception
regulation of protein metabolic process
myoblast proliferation
positive regulation of protein kinase B signaling
regulation of calcium ion transport
negative regulation of oocyte development
lung vasculature development
lung lobe morphogenesis
Type I pneumocyte differentiation
Type II pneumocyte differentiation
prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis
prostate gland growth
prostate gland epithelium morphogenesis
prostate gland stromal morphogenesis
negative regulation of androgen receptor signaling pathway
ERK1 and ERK2 cascade
negative regulation of ERK1 and ERK2 cascade
positive regulation of calcineurin-NFAT signaling cascade
positive regulation of protein serine/threonine kinase activity
positive regulation of steroid hormone biosynthetic process
negative regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of neuron death
positive regulation of trophectodermal cell proliferation
positive regulation of myoblast proliferation
negative regulation of extrinsic apoptotic signaling pathway
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