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          • Primary Antibodies ›
          • IGF1 Antibodies

          Invitrogen

          IGF1 Recombinant Rabbit Monoclonal Antibody (8W6E4)

          View all (39) IGF1 antibodies

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          Cite IGF1 Recombinant Rabbit Monoclonal Antibody (8W6E4)

          • Antibody Testing Data (3)
          IGF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          IGF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          IGF1 Antibody (MA5-35060) in IHC (P)

          Immunohistochemistry analysis of IGF1 in paraffin-embedded human placenta. Samples were incubated with IGF1 Monoclonal antibody (Product # MA5-35060) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          IGF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          IGF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          IGF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

          Product Details

          MA5-35060

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          8W6E4

          Immunogen

          A synthetic peptide corresponding to a sequence within amino acids 70-150 of human IGF1 (P05019)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.35 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2848965

          Product Specific Information

          Immunogen sequence: GFYFNKPTGY GSSSRRAPQT GIVDECCFRS CDLRRLEMYC APLKPAKSAR SVRAQRHTDM PKTQKYQPPS TNKNTKSQRR K

          Target Information

          IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1A; IGFIa; Insulin like growth factor; Insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); Insulin-like growth factor I; insulin-like growth factor IB; M-IGF-1; Mechano growth factor; MGF; OTTHUMP00000195084; R-IGF-1; Somatomedin; Somatomedin C; Somatomedin-C

          View more View less

          Gene Aliases: C730016P09Rik; IBP1; IGF-1; IGF-I; IGF1; IGFI; MGF

          View more View less

          UniProt ID: (Human) P05019, (Mouse) P05017

          View more View less

          Entrez Gene ID: (Human) 3479, (Mouse) 16000

          View more View less

          Function(s)
          insulin receptor binding insulin-like growth factor receptor binding integrin binding hormone activity steroid binding protein binding growth factor activity protein serine/threonine kinase activator activity
          Process(es)
          activation of MAPK activity skeletal system development response to hypoxia cell activation platelet degranulation DNA replication regulation of translation movement of cell or subcellular component signal transduction Ras protein signal transduction muscle organ development female pregnancy aging response to nutrient memory cell proliferation positive regulation of cell proliferation response to heat glycolate metabolic process regulation of gene expression positive regulation of glycoprotein biosynthetic process positive regulation of cardiac muscle hypertrophy phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling response to activity skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration muscle hypertrophy myotube cell development positive regulation of smooth muscle cell migration proteoglycan biosynthetic process positive regulation of cell migration response to caffeine activation of protein kinase B activity response to lipopolysaccharide cellular response to insulin stimulus positive regulation of protein import into nucleus, translocation negative regulation of smooth muscle cell apoptotic process response to nicotine bone mineralization involved in bone maturation regulation of multicellular organism growth positive regulation of activated T cell proliferation positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of apoptotic process positive regulation of DNA binding positive regulation of MAPK cascade protein kinase B signaling positive regulation of insulin-like growth factor receptor signaling pathway cellular protein metabolic process myoblast differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of Ras protein signal transduction insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of peptidyl-tyrosine phosphorylation protein stabilization detection of mechanical stimulus involved in sensory perception response to glucocorticoid myoblast proliferation regulation of calcium ion transport negative regulation of oocyte development positive regulation of cell growth involved in cardiac muscle cell development ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to electrical stimulus cellular response to estradiol stimulus negative regulation of release of cytochrome c from mitochondria negative regulation of neuron death positive regulation of trophectodermal cell proliferation negative regulation of cholangiocyte apoptotic process positive regulation of transcription regulatory region DNA binding negative regulation of extrinsic apoptotic signaling pathway regulation of protein phosphorylation blood vessel remodeling nervous system development negative regulation of cell proliferation glial cell differentiation positive regulation of cerebellar granule cell precursor proliferation water homeostasis positive regulation of cell growth lung development mammary gland development exocrine pancreas development regulation of establishment or maintenance of cell polarity regulation of intracellular steroid hormone receptor signaling pathway multicellular organism growth regulation of cell proliferation negative regulation of cysteine-type endopeptidase activity involved in apoptotic process lung alveolus development cell development branching morphogenesis of an epithelial tube inner ear development chondroitin sulfate proteoglycan biosynthetic process regulation of protein metabolic process positive regulation of protein kinase B signaling lung vasculature development lung lobe morphogenesis Type I pneumocyte differentiation Type II pneumocyte differentiation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis prostate gland growth prostate gland epithelium morphogenesis prostate gland stromal morphogenesis negative regulation of androgen receptor signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity positive regulation of steroid hormone biosynthetic process extrinsic apoptotic signaling pathway in absence of ligand positive regulation of myoblast proliferation
          It has to be done as per old AB suggested Products section.
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