Proteintech

IGF2BP3 Polyclonal Antibody

IGF2BP3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

IGF2BP3 Antibody (14642-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

IGF2BP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IGF2BP3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14642-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 54 publications 54 publications

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 14 publications 14 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IGF2BP3 Fusion Protein Ag6226 (240-579 aa encoded by BC065269)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Both isoforms (64 kDa and 22 kDa) of IGFBP3 can be recognized by this antibody. IGFBP3 is nuclear and cytoplasmic in staining.

Immunogen sequence: AEKSITILS TPEGTSAACK SILEIMHKEA QDIKFTEEIP LKILAHNNFV GRLIGKEGRN LKKIEQDTDT KITISPLQEL TLYNPERTIT VKGNVETCAK AEEEIMKKIR ESYENDIASM NLQAHLIPGL NLNALGLFPP TSGMPPPTSG PPSAMTPPYP QFEQSETETV HLFIPALSVG AIIGKQGQHI KQLSRFAGAS IKIAPAEAPD AKVRMVIITG PPEAQFKAQG RIYGKIKEEN FVSPKEEVKL EAHIRVPSFA AGRVIGKGGK TVNELQNLSS AEVVVPRDQT PDENDQVVVK ITGHFYACQV AQRKIQEILT QVKQHQQQKA LQSGPPQSRR K (240-579 aa encoded by BC065269)

Target Information

Insulin-like growth factor-II messenger RNA (mRNA)-binding protein-3 (IMP-3), also known as K homology domain-containing protein overexpressed in cancer (KOC) and L523S, is a member of the insulin-like growth factor-II mRNA-binding protein family and is expressed during embryogenesis and in some malignancies. IMP-3 is expressed in malignant melanoma but not in benign nevi, even when dysplastic features are present; IMP-3 is expressed in a significantly higher proportion of melanomas than Spitz nevi; and IMP-3 is expressed in metastatic melanomas significantly more than in thin melanomas. IMP-3 appears to be involved in the progression of malignant melanoma and may play an important role in the regulation of the biologic behavior of this tumor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The miR-27a-3p/FTO axis modifies hypoxia-induced malignant behaviors of glioma cells.

Acta biochimica et biophysica Sinica

Du P,Meng L,Liao X,Liu Y,Mo X,Gong M,Liao Y

Wed Jan 25 00:00:00 UTC 2023

Multi-omics analysis of N6-methyladenosine reader IGF2BP3 as a promising biomarker in pan-cancer.

Frontiers in immunology

Chen P,Xu J,Cui Z,Wu S,Xie T,Zhang X

Mon Feb 13 00:00:00 UTC 2023

Multi-omics analysis of N6-methyladenosine reader IGF2BP3 as a promising biomarker in pan-cancer.

Frontiers in immunology

Chen P,Xu J,Cui Z,Wu S,Xie T,Zhang X

Mon Feb 13 00:00:00 UTC 2023

UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma.

International journal of oncology

Fu W,Lei X,Lu Q,Zhang J,Guo J,Zhao J,Tong X,Hu X

Sat Apr 01 00:00:00 UTC 2023

Sirtuin 6 is required for the integrated stress response and resistance to inhibition of transcriptional cyclin-dependent kinases.

Science translational medicine

Kartha N,Gianopulos JE,Schrank Z,Cavender SM,Dobersch S,Kynnap BD,Wallace-Povirk A,Wladyka CL,Santana JF,Kim JC,Yu A,Bridgwater CM,Fuchs K,Dysinger S,Lampano AE,Notta F,Price DH,Hsieh AC,Hingorani SR,Kugel S

Wed May 03 00:00:00 UTC 2023

Ascorbic acid protects retinal pigment epithelial cells from high glucose by inhibiting the NF-κB signal pathway through MALAT1/IGF2BP3 axis.

Diabetic medicine : a journal of the British Diabetic Association

Tian Y,Cheng W,Wang H,Zeng C,Chen X

Mon May 01 00:00:00 UTC 2023

METTL3 regulates alternative splicing of cell cycle-related genes via crosstalk between mRNA m⁶A modifications and splicing factors.

American journal of cancer research

Kim Y,Shin S,Kwon S,Moon K,Baek SV,Jo A,Kim HS,Hwang GH,Bae S,Kim YH,Cho SY,Oh JM

Sun May 14 00:00:00 UTC 2023

Expression pattern analysis of m6A regulators reveals IGF2BP3 as a key modulator in osteoarthritis synovial macrophages.

Journal of translational medicine

Lu Y,Zhang H,Pan H,Zhang Z,Zeng H,Xie H,Yin J,Tang W,Lin R,Zeng C,Cai D

Mon May 22 00:00:00 UTC 2023

Expression pattern analysis of m6A regulators reveals IGF2BP3 as a key modulator in osteoarthritis synovial macrophages.

Journal of translational medicine

Lu Y,Zhang H,Pan H,Zhang Z,Zeng H,Xie H,Yin J,Tang W,Lin R,Zeng C,Cai D

Mon May 22 00:00:00 UTC 2023

Expression pattern analysis of m6A regulators reveals IGF2BP3 as a key modulator in osteoarthritis synovial macrophages.

Journal of translational medicine

Lu Y,Zhang H,Pan H,Zhang Z,Zeng H,Xie H,Yin J,Tang W,Lin R,Zeng C,Cai D

Mon May 22 00:00:00 UTC 2023

Expression pattern analysis of m6A regulators reveals IGF2BP3 as a key modulator in osteoarthritis synovial macrophages.

Journal of translational medicine

Lu Y,Zhang H,Pan H,Zhang Z,Zeng H,Xie H,Yin J,Tang W,Lin R,Zeng C,Cai D

Mon May 22 00:00:00 UTC 2023

NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer.

Apoptosis : an international journal on programmed cell death

Wang Y,Zhang L,Sun XL,Lu YC,Chen S,Pei DS,Zhang LS

Wed Feb 01 00:00:00 UTC 2023

N⁶-methyladenosine of Spi2a attenuates inflammation and sepsis-associated myocardial dysfunction in mice.

Nature communications

Wang X,Ding Y,Li R,Zhang R,Ge X,Gao R,Wang M,Huang Y,Zhang F,Zhao B,Liao W,Du J

Thu Mar 02 00:00:00 UTC 2023

Methyltransferase-like 3 promotes cervical cancer metastasis by enhancing cathepsin L mRNA stability in an N6-methyladenosine-dependent manner.

Cancer science

Liu P,Ju M,Zheng X,Jiang Y,Yu X,Pan B,Luo R,Jia W,Zheng M

Wed Mar 01 00:00:00 UTC 2023

Characterization of circSCL38A1 as a novel oncogene in bladder cancer via targeting ILF3/TGF-β2 signaling axis.

Cell death & disease

Li P,Mi Q,Yan S,Xie Y,Cui Z,Zhang S,Wang Y,Gao H,Wang Y,Li J,Du L,Wang C

Wed Jan 25 00:00:00 UTC 2023

RNA N6-methyladenosine reader IGF2BP3 interacts with MYCN and facilitates neuroblastoma cell proliferation.

Cell death discovery

Zhu K,Gao T,Wang Z,Zhang L,Tan K,Lv Z

Mon May 08 00:00:00 UTC 2023

RNA N6-methyladenosine reader IGF2BP3 interacts with MYCN and facilitates neuroblastoma cell proliferation.

Cell death discovery

Zhu K,Gao T,Wang Z,Zhang L,Tan K,Lv Z

Mon May 08 00:00:00 UTC 2023

RNA N6-methyladenosine reader IGF2BP3 interacts with MYCN and facilitates neuroblastoma cell proliferation.

Cell death discovery

Zhu K,Gao T,Wang Z,Zhang L,Tan K,Lv Z

Mon May 08 00:00:00 UTC 2023

RNA N6-methyladenosine reader IGF2BP3 interacts with MYCN and facilitates neuroblastoma cell proliferation.

Cell death discovery

Zhu K,Gao T,Wang Z,Zhang L,Tan K,Lv Z

Mon May 08 00:00:00 UTC 2023

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 UTC 2023

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 UTC 2023

Comprehensive transcriptomic profiling and mutational landscape of primary gastric linitis plastica.

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association

Liu Z,Hong LL,Zheng JS,Ling ZN,Zhang ZL,Qi YN,Zhang XY,Zhu TY,Wang JL,Han J,Chen XL,Yu QM,Wang S,Li P,Ling ZQ

Wed Mar 01 00:00:00 UTC 2023

Comprehensive transcriptomic profiling and mutational landscape of primary gastric linitis plastica.

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association

Liu Z,Hong LL,Zheng JS,Ling ZN,Zhang ZL,Qi YN,Zhang XY,Zhu TY,Wang JL,Han J,Chen XL,Yu QM,Wang S,Li P,Ling ZQ

Wed Mar 01 00:00:00 UTC 2023

N⁶-methyladenosine reader IGF2BP3 as a prognostic Biomarker contribute to malignant progression of glioma.

Translational cancer research

Zheng X,Li S,Yu J,Dai C,Yan S,Chen G,Sun C

Fri Apr 28 00:00:00 UTC 2023

Comprehensive analyses of molecular features, prognostic values, and regulatory functionalities of m⁶A-modified long non-coding RNAs in lung adenocarcinoma.

Clinical epigenetics

Ping Y,Huang J,Zhu J,Sun Z,Shang A,Chen C,Liu W,Li D

Fri Apr 07 00:00:00 UTC 2023

The G allele of SNP rs3922 reduces the binding affinity between IGF2BP3 and CXCR5 correlating with a lower antibody production.