Proteintech

IGF2R Polyclonal Antibody

IGF2R Antibody in Immunocytochemistry (ICC/IF)

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IGF2R Antibody (20253-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IGF2R Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IGF2R Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20253-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to IGF2R.

Target Information

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of approximately 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and approximately 10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion.

bioRxiv : the preprint server for biology

Zhang J,Kennedy A,de Melo Jorge DM,Xing L,Reid W,Bui S,Joppich J,Rose M,Ercan S,Tang Q,Tai AW,Wang Y

Fri Mar 15 00:00:00 UTC 2024

Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization.

Metabolism: clinical and experimental

Hui Y,Xu Z,Li J,Kuang L,Zhong Y,Tang Y,Wei J,Zhou H,Zheng T

Sun Jan 01 00:00:00 UTC 2023

Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization.

Metabolism: clinical and experimental

Hui Y,Xu Z,Li J,Kuang L,Zhong Y,Tang Y,Wei J,Zhou H,Zheng T

Sun Jan 01 00:00:00 UTC 2023

A proteomic signature and potential pharmacological opportunities in the adaptive resistance to MEK and PI3K kinase inhibition in pancreatic cancer cells.

Proteomics

Aguilar-Valdés A,González-Vela F,Sánchez-Vidal H,Martínez-Aguilar J

Tue Aug 01 00:00:00 UTC 2023

Synchronized proinsulin trafficking reveals delayed Golgi export accompanies β-cell secretory dysfunction in rodent models of hyperglycemia.

Scientific reports

Boyer CK,Bauchle CJ,Zhang J,Wang Y,Stephens SB

Thu Mar 30 00:00:00 UTC 2023

Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules.

The Journal of cell biology

Parchure A,Tian M,Stalder D,Boyer CK,Bearrows SC,Rohli KE,Zhang J,Rivera-Molina F,Ramazanov BR,Mahata SK,Wang Y,Stephens SB,Gershlick DC,von Blume J

Mon Dec 05 00:00:00 UTC 2022

Identification and characterization of a membrane receptor that binds to human STC1.

Life science alliance

Wan HT,Ng AH,Lee WK,Shi F,Wong CK

Tue Nov 01 00:00:00 UTC 2022

GCAF(TMEM251) regulates lysosome biogenesis by activating the mannose-6-phosphate pathway.

Nature communications

Zhang W,Yang X,Li Y,Yu L,Zhang B,Zhang J,Cho WJ,Venkatarangan V,Chen L,Burugula BB,Bui S,Wang Y,Duan C,Kitzman JO,Li M

Mon Sep 12 00:00:00 UTC 2022

Proteomics analysis of cancer tissues identifies IGF2R as a potential therapeutic target in laryngeal carcinoma.

Frontiers in endocrinology

Liu B,Hu Y,Wan L,Wang L,Cheng L,Sun H,Liu Y,Wu D,Zhu J,Hong X,Li Y,Zhou C

Sat Nov 05 00:00:00 UTC 2022

Trop2 binding IGF2R induces gefitinib resistance in NSCLC by remodeling the tumor microenvironment.

Journal of Cancer

Sun X,Jia L,Wang T,Zhang Y,Zhao W,Wang X,Chen H

Tue Aug 03 00:00:00 UTC 2021

Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation.

Cell death & disease

Zhang Z,Mou Z,Xu C,Wu S,Dai X,Chen X,Ou Y,Chen Y,Yang C,Jiang H

Sat Aug 07 00:00:00 UTC 2021

Rumen-Protected Glucose Stimulates the Insulin-Like Growth Factor System and mTOR/AKT Pathway in the Endometrium of Early Postpartum Dairy Cows.

Animals : an open access journal from MDPI

Wang Y,Han X,Tan Z,Kang J,Wang Z

Sun Feb 23 00:00:00 UTC 2020

Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.

Molecular biology of the cell

Ireland SC,Huang H,Zhang J,Li J,Wang Y

Sat Aug 01 00:00:00 UTC 2020

Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.

Molecular biology of the cell

Ireland SC,Huang H,Zhang J,Li J,Wang Y

Sat Aug 01 00:00:00 UTC 2020

Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation.

Journal of the science of food and agriculture

Yang S,Zhao N,Sun B,Yang Y,Hu Y,Zhao R

Sun Mar 15 00:00:00 UTC 2020

Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation.

Journal of the science of food and agriculture

Yang S,Zhao N,Sun B,Yang Y,Hu Y,Zhao R

Sun Mar 15 00:00:00 UTC 2020

Mitotically Stable Modification of DNA Methylation in IGF2/H19 Imprinting Control Region Is Associated with Activated Hepatic IGF2 Expression in Offspring Rats from Betaine-Supplemented Dams.

Journal of agricultural and food chemistry

Yang S,Zhao N,Yang Y,Hu Y,Dong H,Zhao R

Wed Mar 21 00:00:00 UTC 2018

Mitotically Stable Modification of DNA Methylation in IGF2/H19 Imprinting Control Region Is Associated with Activated Hepatic IGF2 Expression in Offspring Rats from Betaine-Supplemented Dams.

Journal of agricultural and food chemistry

Yang S,Zhao N,Yang Y,Hu Y,Dong H,Zhao R

Wed Mar 21 00:00:00 UTC 2018

Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.

Human molecular genetics

Tanaka Y,Suzuki G,Matsuwaki T,Hosokawa M,Serrano G,Beach TG,Yamanouchi K,Hasegawa M,Nishihara M

Wed Mar 01 00:00:00 UTC 2017

Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.

Human molecular genetics

Tanaka Y,Suzuki G,Matsuwaki T,Hosokawa M,Serrano G,Beach TG,Yamanouchi K,Hasegawa M,Nishihara M

Wed Mar 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: 300 kDa mannose 6-phosphate receptor; Cation-independent mannose-6-phosphate receptor; CD222; CI Man-6-P receptor; IGF-II receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; M6P/IGF2 receptor; M6P/IGF2R; MPR 300

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Gene Aliases: CD222; CI-M6PR; CIMPR; IGF2R; M6P-R; M6P/IGF2R; MPR 300; MPR1; MPR300; MPRI

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UniProt ID: (Human) P11717

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Entrez Gene ID: (Human) 3482

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Function(s)

glycoprotein binding G-protein alpha-subunit binding retinoic acid binding receptor activity G-protein coupled receptor activity insulin-like growth factor-activated receptor activity transporter activity protein binding mannose binding enzyme binding insulin-like growth factor II binding identical protein binding phosphoprotein binding

Process(es)

liver development receptor-mediated endocytosis signal transduction G-protein coupled receptor signaling pathway spermatogenesis post-embryonic development organ regeneration response to retinoic acid positive regulation of apoptotic process insulin-like growth factor receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IGF2R Polyclonal Antibody

IGF2R Antibody (20253-1-AP) in ICC/IF

Product Details

20253-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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