Proteintech

IGFBP3 Polyclonal Antibody

View all (36) IGFBP3 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (9)

IGFBP3 Antibody in Immunocytochemistry (ICC/IF)

IGFBP3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

IGFBP3 Antibody (10189-2-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 10189-2-AP (IGFBP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IGFBP3 Antibody in Immunocytochemistry (ICC/IF)

IGFBP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IGFBP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IGFBP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IGFBP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IGFBP3 Antibody in Western Blot (WB)

IGFBP3 Antibody in Western Blot (WB)

IGFBP3 Antibody in Western Blot (WB)

IGFBP3 Antibody in Western Blot (WB)

Product Image Gallery

IGFBP3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 10189-2-AP (IGFBP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IGFBP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens).

IGFBP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens).

IGFBP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens).

IGFBP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens).

IGFBP3 Antibody in Western Blot (WB) — Mouse colon tissue were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IGFBP3 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 10189-2-AP ( IGFBP3 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

IGFBP3 Antibody in Western Blot (WB) — A2780 cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

IGFBP3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Product Details

10189-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:500

View 20 publications 20 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

-

View 1 publication 1 publication

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Chicken, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IGFBP3 Fusion Protein Ag0242 (134-291 aa encoded by BC000013)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PGNASESEE DRSAGSVESP SVSSTHRVSD PKFHPLHSKI IIIKKGHAKD SQRYKVDYES QSTDTQNFSS ESKRETEYGP CRREMEDTLN HLKFLNVLSP RGVHIPNCDK KGFYKKKQCR PSKGRKRGFC WCVDKYGQPL PGYTTKGKED VHCYSMQSK (134-291 aa encoded by BC000013)

Target Information

IGFBP3 is a gene that belongs to the insulin-like growth factor binding protein (IGFBP) family. The gene encodes a protein that contains both an IGFBP domain and a thyroglobulin type-I domain. This protein is known to form a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. The complex circulates in the plasma and prolongs the half-life of IGFs while also altering their interaction with cell surface receptors. Various isoforms of the gene have been identified through alternate transcriptional splice variants. Diseases associated with IGFBP3 include Insulin-Like Growth Factor I and Acid-Labile Subunit Deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; BP53; Growth hormone dependent binding protein; growth hormone-dependent binding protein; IBP-3; IGF binding protein 3; IGF-binding protein 3; IGFBP; IGFBP-3; Insulin like growth factor binding protein; insulin-like growth factor binding protein 3; Insulin-like growth factor binding protein 3 precursor; Insulin-like growth factor-binding protein 3; tcag7.703

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Gene Aliases: AI649005; BP-53; IBP3; IGFBP-3; IGFBP3; IGgfbp3

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UniProt ID: (Human) P17936, (Mouse) P47878

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Entrez Gene ID: (Human) 3486, (Mouse) 16009

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fibronectin binding protein binding insulin-like growth factor binding protein tyrosine phosphatase activator activity insulin-like growth factor I binding insulin-like growth factor II binding metal ion binding growth factor binding

regulation of cell growth negative regulation of protein phosphorylation protein phosphorylation negative regulation of cell proliferation regulation of glucose metabolic process negative regulation of smooth muscle cell migration regulation of growth positive regulation of apoptotic process positive regulation of catalytic activity positive regulation of MAPK cascade positive regulation of insulin-like growth factor receptor signaling pathway type B pancreatic cell proliferation positive regulation of myoblast differentiation negative regulation of smooth muscle cell proliferation osteoblast differentiation apoptotic process negative regulation of signal transduction regulation of apoptotic process regulation of insulin-like growth factor receptor signaling pathway cellular protein metabolic process

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