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Invitrogen
IKK epsilon Recombinant Rabbit Monoclonal Antibody (5N4J8)
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FIGURE: 1 / 3
IKK epsilon Antibody (MA5-35371) in IHC (P)
Immunohistochemistry analysis of IKK epsilon in paraffin-embedded human placenta. Samples were incubated with IKK epsilon Monoclonal antibody (Product # MA5-35371) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
MA5-35371
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5N4J8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IKKε (Q14164)
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849272
Product Specific Information
Immunogen sequence: MQSTANYLWH TDDLLGQGAT ASVYKARNKK SGELVAVKVF NTTSYLRPRE VQVREFEVLR KLNHQNIVKL FAVEETGGSR QKVLVMEYCS SGSLLSVLES
Target Information
IKBKE is an activator of nuclear factor-kappa-B. Over expression of IKBKE has been observed in breast cancer cells, and suppression of IKBKE induces cell death. NF-kappa-B is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Ikappa-B is phosphorylated by Ikappa-B kinase (IKK) complex that contains IKK-a, IKK-b, and IKK-gamma. A novel molecule in the IKK complex was recently identified and designated IKK-iota/IKK-i. IKK epsilon is required for the activation of NF-kappa-B by PMA and T cell receptors but not by TNF-a and IL-1. IKK-iota/IKK-i message is expressed in a variety of tissues and is inducible by TNF-a, IL-1, and LPS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IKK-i; IKK-related kinase epsilon; Inducible I kappa-B kinase; inducible IKappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of kappaB kinase epsilon; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; RP11-534L20.1
Gene Aliases: AW558201; IKBKE; IKK-E; IKK-i; IKKE; IKKepsilon; IKKI; KIAA0151
UniProt ID: (Human) Q14164, (Mouse) Q9R0T8
Entrez Gene ID: (Human) 9641, (Rat) 363984, (Mouse) 56489
protein kinase activity
NF-kappaB-inducing kinase activity
protein binding
ATP binding
IkappaB kinase activity
ubiquitin protein ligase binding
K48-linked polyubiquitin binding
K63-linked polyubiquitin binding
nucleotide binding
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein phosphorylation
I-kappaB phosphorylation
intrinsic apoptotic signaling pathway in response to DNA damage
NIK/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
immune response
negative regulation of type I interferon production
response to type I interferon
response to interferon-beta
TRIF-dependent toll-like receptor signaling pathway
protein homooligomerization
cellular response to virus
cellular response to DNA damage stimulus
phosphorylation
signal transduction by protein phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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