Proteintech

IL 1 alpha Polyclonal Antibody

IL 1 alpha Antibody in Immunocytochemistry (ICC/IF)

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IL 1 alpha Antibody (16765-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed RAW 264.7 cells using 16765-1-AP (IL 1 alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IL 1 alpha Antibody in Western Blot (WB)

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Product Details

16765-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

ELISA (ELISA)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IL 1 alpha Fusion Protein Ag10339 (1-271 aa encoded by BC013142)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAKVPDMFE DLKNCYSENE EDSSSIDHLS LNQKSFYHVS YGPLHEGCMD QSVSLSISET SKTSKLTFKE SMVVVATNGK VLKKRRLSLS QSITDDDLEA IANDSEEEII KPRSAPFSFL SNVKYNFMRI IKYEFILNDA LNQSIIRAND QYLTAAALHN LDEAVKFDMG AYKSSKDDAK ITVILRISKT QLYVTAQDED QPVLLKEMPE IPKTITGSET NLLFFWETHG TKNYFTSVAH PNLFIATKQD YWVCLAGGPP SITDFQILEN QA (1-271 aa encoded by BC013142)

Target Information

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CTLA8; Hematopoietin-1; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 1, alpha; interleukin 17; Interleukin-1 alpha; Interleukin-17A; Interleukin1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha; RP23-160G19.8

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Gene Aliases: IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1

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UniProt ID: (Human) P01583, (Mouse) P01582

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Entrez Gene ID: (Human) 3552, (Mouse) 16175

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Function(s)

cytokine activity interleukin-1 receptor binding copper ion binding protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL 1 alpha Polyclonal Antibody

IL 1 alpha Antibody (16765-1-AP) in ICC/IF

Product Details

16765-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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