Invitrogen

IL-1 alpha Monoclonal Antibody (ALF-161), PE, eBioscience™

IL-1 alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

IL-1 alpha Antibody (12-7011-82) in Flow

BALB/c mouse thioglycolate-elicited peritoneal exudate cells were unstimulated (left) or stimulated overnight with LPS in presence of Brefeldin A (Product # 00-4976-03 and Product # 00-4506-51) and IFN gamma (Product # BMS326) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD11b Monoclonal Antibody, APC (Product # 17-0112-82) and 0.25 µg of IL-1 alpha Monoclonal Antibody, PE. Total viable cells were used for analysis.

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Product Details

12-7011-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ALF-161

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_466144

Product Specific Information

Description: The ALF-161 antibody reacts with mouse interleukin-1alpha (IL-1alpha). Mouse IL-1alpha, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported:The ALF-161 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometry.

Applications Tested: Has been pre-titrated can be used at test size: 5 µL (0.25µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

TLR7 controls myeloid-derived suppressor cells expansion and function in the lung of C57BL6 mice infected with Schistosoma japonicum.

PLoS neglected tropical diseases

Zhou L,Zhu Y,Mo L,Wang M,Lin J,Zhao Y,Feng Y,Xie A,Wei H,Qiu H,Huang J,Yang Q

12-7011-82 was used in Flow cytometry/Cell sorting to indicate that TLR7 signaling inhibits the accumulation and function of MDSCs in S. japonicum infected mouse lung by down-regulating the expression of PD-L1/2 and secreting of IL-10, via NF-κB signaling.

Sat Oct 01 00:00:00 UTC 2022

Legionella-Infected Macrophages Engage the Alveolar Epithelium to Metabolically Reprogram Myeloid Cells and Promote Antibacterial Inflammation.

Cell host & microbe

Liu X,Boyer MA,Holmgren AM,Shin S

12-7011-82 was used in Flow Cytometry to reveal that alveolar macrophages engage alveolar epithelial signals to metabolically reprogram monocytes for antibacterial inflammation.

Wed Nov 11 00:00:00 UTC 2020

Blockade of MK2 is protective in inflammation-associated colorectal cancer development.

International journal of cancer

Ray AL,Castillo EF,Morris KT,Nofchissey RA,Weston LL,Samedi VG,Hanson JA,Gaestel M,Pinchuk IV,Beswick EJ

12-7011 was used in Flow cytometry/Cell sorting to investigate the role of MAPK-activated protein kinase 2 in development of colitis-associated colon cancer.

Mon Feb 01 00:00:00 UTC 2016

IL-1α and IL-1β recruit different myeloid cells and promote different stages of sterile inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Rider P,Carmi Y,Guttman O,Braiman A,Cohen I,Voronov E,White MR,Dinarello CA,Apte RN

Tue Nov 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CTLA8; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin-17A; Interleukin1 alpha; RP23-160G19.8

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Gene Aliases: Il-1a; Il1a

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UniProt ID: (Mouse) P01582

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Entrez Gene ID: (Mouse) 16175

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Function(s)

cytokine activity interleukin-1 receptor binding copper ion binding

Process(es)

Performance Guarantee

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IL-1 alpha Monoclonal Antibody (ALF-161), PE, eBioscience™

IL-1 alpha Antibody (12-7011-82) in Flow

Product Details

12-7011-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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