Proteintech

IL-1 beta Polyclonal Antibody

FIGURE: 1 / 2

IL-1 beta Antibody (26048-1-AP) in WB

Untreated, or RAW 264.7 cells treated with100 ng/mL LPS for 3 hours and 300 ng/mL Brefeldin A for the last 3 hours, were subjected to SDS PAGE followed by western blot with (Product # 26048-1-AP) (IL-1 beta antibody) at dilution of 1:1,500 incubated at room temperature for 1.5 hours.

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Product Details

26048-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 42 publications 42 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

ELISA (ELISA)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IL-1B Fusion Protein Ag23461

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

850 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

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Gene Aliases: Il-1b; IL-1beta; Il1b

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UniProt ID: (Mouse) P10749

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Entrez Gene ID: (Mouse) 16176

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Function(s)

receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding

Process(es)

Performance Guarantee

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IL-1 beta Polyclonal Antibody

IL-1 beta Antibody (26048-1-AP) in WB

Product Details

26048-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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