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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • IL-1 beta Antibodies

          Invitrogen

          IL-1 beta Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (119) IL-1 beta antibodies

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          Datasheet
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          Datasheet
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          Cite IL-1 beta Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (1)
          IL-1 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          IL-1 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          IL-1 beta Antibody (PA5-79485) in IHC (P)

          Immunohistochemistry analysis of IL-1 beta in paraffin-embedded section of mouse spleen tissue. Samples were incubated with IL-1 beta polyclonal antibody (Product # PA5-79485) using a dilution of 2 μg/mL overnight at 4°C followed by Peroxidase Conjugated Goat Anti-rabbit IgG incubated for 30 minutes at 37°C. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). T... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          IL-1 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          IL-1 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          IL-1 beta Antibody in Western Blot (WB)
          IL-1 beta Antibody in Western Blot (WB)
          IL-1 beta Antibody in Western Blot (WB)

          Product Details

          PA5-79485

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          2-5 µg/mL
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived rat IL-1 beta recombinant protein (Position: V117-S268).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2746601

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: mouse RAW2647 whole cell, mouse RAW2647 whole cell. IHC: mouse spleen tissue, rat spleen tissue.

          Target Information

          Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

          View more View less

          Gene Aliases: Il-1b; IL-1beta; Il1b

          View more View less

          UniProt ID: (Mouse) P10749

          View more View less

          Entrez Gene ID: (Mouse) 16176, (Rat) 24494

          View more View less

          Function(s)
          receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity fever generation positive regulation of protein phosphorylation inflammatory response immune response positive regulation of cytosolic calcium ion concentration aging memory negative regulation of cell proliferation response to carbohydrate positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of glucose transport positive regulation of cell death negative regulation of glutamate secretion cytokine-mediated signaling pathway hyaluronan biosynthetic process neutrophil chemotaxis sequestering of triglyceride positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to lipopolysaccharide interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of stress-activated MAPK cascade positive regulation of immature T cell proliferation in thymus response to ATP positive regulation of heterotypic cell-cell adhesion social behavior ectopic germ cell programmed cell death positive regulation of myosin light chain kinase activity cellular response to drug positive regulation of T cell proliferation positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of chemokine biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of astrocyte differentiation positive regulation of phagocytosis negative regulation of neurogenesis regulation of insulin secretion leukocyte migration negative regulation of lipid catabolic process positive regulation of lipid catabolic process positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of cell division positive regulation of calcidiol 1-monooxygenase activity negative regulation of adiponectin secretion positive regulation of ERK1 and ERK2 cascade monocyte aggregation cellular response to organic substance cellular response to organic cyclic compound positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation negative regulation of extrinsic apoptotic signaling pathway in absence of ligand response to hypoxia chronic inflammatory response to antigenic stimulus purine nucleobase metabolic process response to nutrient learning or memory estrogen metabolic process glycoprotein metabolic process response to heat response to ozone response to organonitrogen compound response to gamma radiation response to organic cyclic compound pentacyclic triterpenoid metabolic process polyketide metabolic process ovulation response to estradiol response to vitamin D response to L-ascorbic acid response to stilbenoid response to diuretic response to statin wound healing response to drug response to morphine response to peptide hormone response to ethanol response to glucocorticoid cellular response to lipopolysaccharide cellular response to antibiotic cellular response to mechanical stimulus cellular response to glucose stimulus cellular response to lipid cellular response to fatty acid cellular response to methotrexate response to dexamethasone
          It has to be done as per old AB suggested Products section.
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