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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • IL-1 beta Antibodies

          Invitrogen

          IL-1 beta Monoclonal Antibody (CRM56), FITC, eBioscience™

          7 Published Figures
          7 References
          View all (119) IL-1 beta antibodies

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          Datasheet
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          Cite IL-1 beta Monoclonal Antibody (CRM56), FITC, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (7)
          IL-1 beta Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          IL-1 beta Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          IL-1 beta Antibody (11-7018-42) in Flow

          Normal human peripheral blood cells were stimulated for 4 hours with LPS (Product # 00-4976-03) in the presence of Brefeldin A (Product # 00-4506-51). Cells were then surface stained with CD14 Monoclonal Antibody, APC (Product # 17-0149-42), followed by intracellular staining using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with and Mouse IgG1 kappa Isotype Control, FITC (Product # 11-4714-82) (left) or CRM56 Monoclonal Antibody, FITC (right). Cells in the monocyte gate were used for analy... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          IL-1 beta Antibody in Flow Cytometry (Flow)
          IL-1 beta Antibody in Flow Cytometry (Flow)
          IL-1 beta Antibody in Flow Cytometry (Flow)
          IL-1 beta Antibody in Flow Cytometry (Flow)
          IL-1 beta Antibody in Flow Cytometry (Flow)
          IL-1 beta Antibody in Flow Cytometry (Flow)
          IL-1 beta Antibody in Neutralization (Neu)
          IL-1 beta Antibody in Neutralization (Neu)

          Product Details

          11-7018-42

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (0.125 µg)/test
          View 6 publications 6 publications

          Neutralization (Neu)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Mouse / IgG1

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          CRM56

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • Alexa Fluor 647 
          • Brilliant UV 395
          • Brilliant Violet 650
          • Functional Grade
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10598802

          Product Specific Information

          Description: The CRM56 antibody reacts with human and baboon interleukin-1 beta; (IL-1 beta). IL-1 beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.

          Applications Reported: The CRM56 antibody has been reported for use in intracellular flow cytometric analysis.

          Applications Tested: This CRM56 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1, beta; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta

          View more View less

          Gene Aliases: IL-1; IL1-BETA; IL1B; IL1F2

          View more View less

          UniProt ID: (Human) P01584

          View more View less

          Entrez Gene ID: (Human) 3553

          View more View less

          Function(s)
          cytokine activity interleukin-1 receptor binding protein domain specific binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity fever generation response to hypoxia positive regulation of protein phosphorylation chronic inflammatory response to antigenic stimulus positive regulation of T cell mediated immunity purine nucleobase metabolic process apoptotic process inflammatory response signal transduction positive regulation of cytosolic calcium ion concentration cell-cell signaling embryo implantation aging memory estrogen metabolic process negative regulation of cell proliferation glycoprotein metabolic process response to heat response to ozone response to gamma radiation positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of glucose transport negative regulation of glutamate secretion smooth muscle adaptation cytokine-mediated signaling pathway pentacyclic triterpenoid metabolic process hyaluronan biosynthetic process neutrophil chemotaxis polyketide metabolic process ovulation sequestering of triglyceride positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to estradiol interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of immature T cell proliferation in thymus positive regulation of histone phosphorylation response to ATP response to vitamin D response to L-ascorbic acid positive regulation of heterotypic cell-cell adhesion positive regulation of histone acetylation social behavior ectopic germ cell programmed cell death positive regulation of myosin light chain kinase activity response to stilbenoid cellular response to drug response to diuretic response to statin wound healing positive regulation of T cell proliferation positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling response to morphine negative regulation of MAP kinase activity response to peptide hormone protein kinase B signaling positive regulation of JUN kinase activity positive regulation of chemokine biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol negative regulation of neuron differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of protein export from nucleus positive regulation of astrocyte differentiation positive regulation of phagocytosis regulation of insulin secretion negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of cell division positive regulation of cell adhesion molecule production positive regulation of calcidiol 1-monooxygenase activity negative regulation of adiponectin secretion positive regulation of ERK1 and ERK2 cascade monocyte aggregation cellular response to antibiotic cellular response to mechanical stimulus cellular response to organic substance cellular response to glucose stimulus cellular response to fatty acid cellular response to organic cyclic compound cellular response to methotrexate response to dexamethasone positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand regulation of establishment of endothelial barrier negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation positive regulation of interleukin-6 secretion negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.
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