Invitrogen

IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™

29 Published Figures

38 References

View all (96) IL-10 antibodies

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Protocols

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Published Figures (29)

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IL-10 Antibody (11-7101-41) in Flow

Neural regeneration research 2024 - Fasudil-modified macrophages affect macrophage polarization. A mouse model of chronic EAE was induced by subcutaneous injection of myelin oligodendrocyte glycoprotein peptde 35-55. Macrophages from EAE mice isolated with magnetic beads were treated with 15 mug/mL of Fasudil at 37degC in 5% CO 2 atmosphere for 72 hours. Fasudil-treated macrophages were suspended in PBS at a concentration of 2 x 10 7 cells/20... View More

IL-10 Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

Product Details

11-7101-41

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 35 publications 35 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES5-16E3

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572513

Product Specific Information

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.

Applications Tested: This JES5-16E3 antibody has been pre-titrated and tested by intracellular staining for flow cytometric analysis. test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of LPS-stimulated normal human peripheral blood cells. This can be used at test size: 5 µL (0.25 µg)/test per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2024

Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer.

Cancer immunology, immunotherapy : CII

Jianyi D,Haili G,Bo Y,Meiqin Y,Baoyou H,Haoran H,Fang L,Qingliang Z,Lingfei H

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Sun Jan 01 00:00:00 UTC 2023

Myeloid cell-specific topoisomerase 1 inhibition using DNA origami mitigates neuroinflammation.

EMBO reports

Zhu K,Wang Y,Sarlus H,Geng K,Nutma E,Sun J,Kung SY,Bay C,Han J,Min JH,Benito-Cuesta I,Lund H,Amor S,Wang J,Zhang XM,Kutter C,Guerreiro-Cacais AO,Högberg B,Harris RA

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Tue Jul 05 00:00:00 UTC 2022

RORγt phosphorylation protects against T cell-mediated inflammation.

Cell reports

Ma S,Patel SA,Abe Y,Chen N,Patel PR,Cho BS,Abbasi N,Zeng S,Schnabl B,Chang JT,Huang WJM

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Tue Mar 15 00:00:00 UTC 2022

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Mon Nov 01 00:00:00 UTC 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Sat May 01 00:00:00 UTC 2021

Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

Theranostics

Tong L,Hao H,Zhang Z,Lv Y,Liang X,Liu Q,Liu T,Gong P,Zhang L,Cao F,Pastorin G,Lee CN,Chen X,Wang JW,Yi H

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Fri Jan 28 00:00:00 UTC 2022

Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi.

Pathogens (Basel, Switzerland)

Medina-Buelvas DM,Rodríguez-Sosa M,Vega L

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Sat Nov 06 00:00:00 UTC 2021

Blockade of TLR2 and TLR4 Attenuates Inflammatory Response and Parasite Load in Cutaneous Leishmaniasis.

Frontiers in immunology

Carneiro PP,Dórea AS,Oliveira WN,Guimarães LH,Brodskyn C,Carvalho EM,Bacellar O

11-7101-41 was used in Flow Cytometry to show that reduction in oxidative burst, IL-1β, TNF and CXCL9 production were observed.

Mon Dec 13 00:00:00 UTC 2021

Retinoic Acid Induces Functionally Suppressive Foxp3⁺RORγt⁺ T Cells In Vitro.

Frontiers in immunology

Martínez-Blanco M,Lozano-Ojalvo D,Pérez-Rodríguez L,Benedé S,Molina E,López-Fandiño R

11-7101-41 was used in Flow Cytometry to show that under the influence of IL-2 and TGF-β, suppressive Foxp3+RORγt+ T cells that express and secrete IL-17 can be produced in vitro and RA further contributes to stabilize this phenotype.

Thu Oct 21 00:00:00 UTC 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Mon May 10 00:00:00 UTC 2021

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Mon Jun 29 00:00:00 UTC 2020

Memory CD4⁺ T cells enhance B-cell responses to drifting influenza immunization.

European journal of immunology

Gage E,Van Hoeven N,Dubois Cauwelaert N,Larsen SE,Erasmus J,Orr MT,Coler RN

11-7101-41 was used in Flow Cytometry to show that MHC Class II haplotype and T-cell reactivity influences subsequent hemagglutination inhibitory antibody responses.

Fri Feb 01 00:00:00 UTC 2019

c-Maf controls immune responses by regulating disease-specific gene networks and repressing IL-2 in CD4⁺ T cells.

Nature immunology

Gabryšová L,Alvarez-Martinez M,Luisier R,Cox LS,Sodenkamp J,Hosking C,Pérez-Mazliah D,Whicher C,Kannan Y,Potempa K,Wu X,Bhaw L,Wende H,Sieweke MH,Elgar G,Wilson M,Briscoe J,Metzis V,Langhorne J,Luscombe NM,O'Garra A

11-7101 was used in Flow cytometry/Cell sorting to study the role of c-Maf as a regulator of the expression of cytokine-encoding genes.

Tue May 01 00:00:00 UTC 2018

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Neutralization

Fri Dec 01 00:00:00 UTC 2017

Development of effective tumor immunotherapy using a novel dendritic cell-targeting Toll-like receptor ligand.

PloS one

De Silva NH,Akazawa T,Wijewardana V,Inoue N,Oyamada M,Ohta A,Tachibana Y,Wijesekera DPH,Kuwamura M,Nishizawa Y,Itoh K,Izawa T,Hatoya S,Hasegawa T,Yamate J,Inaba T,Sugiura K

11-7101 was used in Flow cytometry/Cell sorting to show that the combined treatment gave rise to emphatic amelioration in DC-based cancer therapy.

Tue Dec 26 00:00:00 UTC 2017

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Tue May 17 00:00:00 UTC 2016

Low-Dose Intestinal Trichuris muris Infection Alters the Lung Immune Microenvironment and Can Suppress Allergic Airway Inflammation.

Infection and immunity

Chenery AL,Antignano F,Burrows K,Scheer S,Perona-Wright G,Zaph C

11-7101 was used in Flow cytometry/Cell sorting to study crosstalk between the intestine and lung during disease, showing that low-dose intestinal Trichuris muris infection alters the lung immune microenvironment.

Mon Feb 01 00:00:00 UTC 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

11-7101 was used in Flow cytometry/Cell sorting to investigate the potential of a previously uncharacterized property of natural human adenovirus infection to dictate, modulate and/or alter the course of HCV infection upon exposure.

Tue Jul 19 00:00:00 UTC 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Tue Jul 19 00:00:00 UTC 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Wed May 25 00:00:00 UTC 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2015

Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner.

Cancer research

Kratochvill F,Gratz N,Qualls JE,Van De Velde LA,Chi H,Kovarik P,Murray PJ

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in ELISA

Wed Jul 01 00:00:00 UTC 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in ELISA

Wed Jul 01 00:00:00 UTC 2015

Dimethyl sulfoxide inhibits spontaneous diabetes and autoimmune recurrence in non-obese diabetic mice by inducing differentiation of regulatory T cells.

Toxicology and applied pharmacology

Lin GJ,Sytwu HK,Yu JC,Chen YW,Kuo YL,Yu CC,Chang HM,Chan DC,Huang SH

11-7101 was used in Flow cytometry/Cell sorting to demonstrate that in vivo DMSO treatment suppresses spontaneous diabetes and autoimmune recurrence in NOD mice.

Thu Jan 15 00:00:00 UTC 2015

Preemptive donor apoptotic cell infusions induce IFN-γ-producing myeloid-derived suppressor cells for cardiac allograft protection.

Journal of immunology (Baltimore, Md. : 1950)

Bryant J,Lerret NM,Wang JJ,Kang HK,Tasch J,Zhang Z,Luo X

11-7101 was used in Flow cytometry/Cell sorting to investigate the role of MDSCs in the graft protection provided by ECDI-SPs, showing that preemptive donor apoptotic cell infusions induce IFN-γ-producing MDSCs.

Sun Jun 15 00:00:00 UTC 2014

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Mon Jan 05 00:00:00 UTC 2015

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Wed Jan 01 00:00:00 UTC 2014

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Wed Jan 01 00:00:00 UTC 2014

Prevention or early cure of type 1 diabetes by intranasal administration of gliadin in NOD mice.

PloS one

Funda DP,Fundova P,Hansen AK,Buschard K

11-7101 was used in Flow cytometry/Cell sorting to investigate whether intranasal (i.n.) administration of gliadin or gluten may arrest the diabetogenic process, showing that i.n. administration of gliadin prevents type 1 diabetes in NOD mice.

Tue Jan 13 00:00:00 UTC 2015

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-41) in Flow Cytometry

Tue Jan 01 00:00:00 UTC 2013

Osteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption in vitro.

PloS one

Buchwald ZS,Kiesel JR,DiPaolo R,Pagadala MS,Aurora R

11-7101 was used in Flow cytometry/Cell sorting to demonstrate the effects of activated CD8 T cell-produced cytokines on osteoclast activity.

Thu Nov 08 00:00:00 UTC 2012

IL-10 regulates viral lung immunopathology during acute respiratory syncytial virus infection in mice.

PloS one

Loebbermann J,Schnoeller C,Thornton H,Durant L,Sweeney NP,Schuijs M,O'Garra A,Johansson C,Openshaw PJ

11-7101 was used in Flow cytometry/Cell sorting to study experimental respiratory syncytial virus infection in IL-10 deficient mice and mice treated with anti-IL-10 receptor antibody.

Mon Jul 23 00:00:00 UTC 2012

Infection with a helminth parasite attenuates autoimmunity through TGF-beta-mediated suppression of Th17 and Th1 responses.

Journal of immunology (Baltimore, Md. : 1950)

Walsh KP,Brady MT,Finlay CM,Boon L,Mills KH

11-7101 was used in Flow cytometry/Cell sorting to provide evidence that parasites can exert bystander immunosuppression of pathogenic T cells that mediate autoimmune diseases.

Sat Aug 01 00:00:00 UTC 2009

Ly49 C/I-dependent NKT cell-derived IL-10 is required for corneal graft survival and peripheral tolerance.

Journal of leukocyte biology

Watte CM,Nakamura T,Lau CH,Ortaldo JR,Stein-Streilein J

11-7101 was used in Flow cytometry/Cell sorting to test if the NK inhibitory receptor functionally contributed to tolerance development.

Tue Apr 01 00:00:00 UTC 2008

Deletion of both ICAM-1 and C3 enhances severity of experimental autoimmune encephalomyelitis compared to C3-deficient mice.

Neuroscience letters

Smith SS,Ludwig M,Wohler JE,Bullard DC,Szalai AJ,Barnum SR

11-7101 was used in Flow cytometry/Cell sorting to examine experimental autoimmune encephalitis in ICAM-1 x C3 double knockout mice.

Fri Sep 12 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

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Gene Aliases: CSIF; Il-10; Il10

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UniProt ID: (Mouse) P18893

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Entrez Gene ID: (Mouse) 16153

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Function(s)

cytokine activity interleukin-10 receptor binding

Process(es)

Performance Guarantee

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IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™

IL-10 Antibody (11-7101-41) in Flow

Product Details

11-7101-41

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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