Invitrogen

IL-10 Monoclonal Antibody (JES5-16E3), Functional Grade, eBioscience™

29 Published Figures

34 References

View all (96) IL-10 antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (29)

FIGURE: 1 / 30

IL-10 Antibody (16-7101-85) in IS

Mouse splenocytes (500,000 cells per well) were activated with LPS (1 µg/mL) for 48 hrs in Mouse IL-10 ELISPOT assay. Control well is medium alone.

IL-10 Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

Product Details

16-7101-85

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 28 publications 28 publications

ELISA (ELISA)

View 3 publications 3 publications

Neutralization (Neu)

Assay-Dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Immunostaining (IS)

1-4 µg/mL

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES5-16E3

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_469225

Product Specific Information

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.

Applications Tested: The Functional Grade purified JES5-16E3 antibody has been tested for ELISPOT capture. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/mL.

The JES5-16E3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-10 (IL-10) in combination with the biotinylated JES5-2A5 (13-7102) antibody for detection and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2024

Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer.

Cancer immunology, immunotherapy : CII

Jianyi D,Haili G,Bo Y,Meiqin Y,Baoyou H,Haoran H,Fang L,Qingliang Z,Lingfei H

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Sun Jan 01 00:00:00 UTC 2023

Myeloid cell-specific topoisomerase 1 inhibition using DNA origami mitigates neuroinflammation.

EMBO reports

Zhu K,Wang Y,Sarlus H,Geng K,Nutma E,Sun J,Kung SY,Bay C,Han J,Min JH,Benito-Cuesta I,Lund H,Amor S,Wang J,Zhang XM,Kutter C,Guerreiro-Cacais AO,Högberg B,Harris RA

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Tue Jul 05 00:00:00 UTC 2022

RORγt phosphorylation protects against T cell-mediated inflammation.

Cell reports

Ma S,Patel SA,Abe Y,Chen N,Patel PR,Cho BS,Abbasi N,Zeng S,Schnabl B,Chang JT,Huang WJM

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Tue Mar 15 00:00:00 UTC 2022

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Mon Nov 01 00:00:00 UTC 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Sat May 01 00:00:00 UTC 2021

Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

Theranostics

Tong L,Hao H,Zhang Z,Lv Y,Liang X,Liu Q,Liu T,Gong P,Zhang L,Cao F,Pastorin G,Lee CN,Chen X,Wang JW,Yi H

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Fri Jan 28 00:00:00 UTC 2022

Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi.

Pathogens (Basel, Switzerland)

Medina-Buelvas DM,Rodríguez-Sosa M,Vega L

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Sat Nov 06 00:00:00 UTC 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Mon May 10 00:00:00 UTC 2021

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Mon Jun 29 00:00:00 UTC 2020

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

16-7101 was used in Flow cytometry/Cell sorting to investigate the role that regulatory T cells (in tumour-draining lymph nodes) play in modulating the malignancy of breast cancer cells for distant metastasis.

Fri Dec 01 00:00:00 UTC 2017

NOX2 deficiency alters macrophage phenotype through an IL-10/STAT3 dependent mechanism: implications for traumatic brain injury.

Journal of neuroinflammation

Barrett JP,Henry RJ,Villapol S,Stoica BA,Kumar A,Burns MP,Faden AI,Loane DJ

16-7101 was used in Neutralization experiments to investigate the signalling pathways that regulate NOX2-dependent macrophage/microglial phenotype switching in the TBI brain.

Fri Mar 24 00:00:00 UTC 2017

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Tue May 17 00:00:00 UTC 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Tue Jul 19 00:00:00 UTC 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Wed May 25 00:00:00 UTC 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2015

Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner.

Cancer research

Kratochvill F,Gratz N,Qualls JE,Van De Velde LA,Chi H,Kovarik P,Murray PJ

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2015

Canonical wnt signaling in dendritic cells regulates Th1/Th17 responses and suppresses autoimmune neuroinflammation.

Journal of immunology (Baltimore, Md. : 1950)

Suryawanshi A,Manoharan I,Hong Y,Swafford D,Majumdar T,Taketo MM,Manicassamy B,Koni PA,Thangaraju M,Sun Z,Mellor AL,Munn DH,Manicassamy S

16-7101 was used in Flow cytometry/Cell sorting to study the factors in the tissue microenvironment and signalling networks critical for programming dendritic cells to control chronic inflammation and promote tolerance, showing that Th1/Th17 responses are regulated by canonical Wnt signalling in dendritic cells and suppresses autoimmune neuroinflammation.

Wed Apr 01 00:00:00 UTC 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in ELISA

Wed Jul 01 00:00:00 UTC 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in ELISA

Wed Jul 01 00:00:00 UTC 2015

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Mon Jan 05 00:00:00 UTC 2015

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2014

CD3 antibody and IL-2 complex combination therapy inhibits atherosclerosis by augmenting a regulatory immune response.

Journal of the American Heart Association

Kasahara K,Sasaki N,Yamashita T,Kita T,Yodoi K,Sasaki Y,Takeda M,Hirata K

16-7101 was used in Flow cytometry/Cell sorting to investigate the inhibition of atherosclerosis by a CD3 antibody and IL-2 complex combination.

Tue Apr 22 00:00:00 UTC 2014

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-10 monoclonal antibody (Product # 16-7101-81) in Flow Cytometry

Tue Jan 01 00:00:00 UTC 2013

Comparative analysis of cancer vaccine settings for the selection of an effective protocol in mice.

Journal of translational medicine

Kalli F,Machiorlatti R,Battaglia F,Parodi A,Conteduca G,Ferrera F,Proietti M,Tardito S,Sanguineti M,Millo E,Fenoglio D,De Palma R,Inghirami G,Filaci G

16-7101 was used in a Functional assay to compare different gp100 vaccination strategies to identify the best strategy to provide a 100% protection against experimental melanoma in a reproducible manner.

Sun May 12 00:00:00 UTC 2013

Gr1(int)CD11b+ myeloid-derived suppressor cells in Mycobacterium tuberculosis infection.

PloS one

Obregón-Henao A,Henao-Tamayo M,Orme IM,Ordway DJ

16-7101 was used in Flow cytometry/Cell sorting to compare the bacterial burden, lung pathology and Gr1(int)CD11b(+) myeloid-derived suppressor cell immune responses in M. tuberculosis infected mice.

Fri Jun 20 00:00:00 UTC 2014

Role of arginase 1 from myeloid cells in th2-dominated lung inflammation.

PloS one

Barron L,Smith AM,El Kasmi KC,Qualls JE,Huang X,Cheever A,Borthwick LA,Wilson MS,Murray PJ,Wynn TA

16-7101 was used in Flow cytometry/Cell sorting to investigate the contribution of arginase 1 to asthma pathogenesis.

Mon Dec 09 00:00:00 UTC 2013

The E3 ubiquitin ligase adaptor Ndfip1 regulates Th17 differentiation by limiting the production of proinflammatory cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Ramon HE,Beal AM,Liu Y,Worthen GS,Oliver PM

16-7101 was used in Flow cytometry/Cell sorting to investigate the role of the E3 ubiquitin ligase adaptor Ndfip1 on Th17 differentiation in mice.

Sun Apr 15 00:00:00 UTC 2012

Novel CCL21-vault nanocapsule intratumoral delivery inhibits lung cancer growth.

PloS one

Kar UK,Srivastava MK,Andersson A,Baratelli F,Huang M,Kickhoefer VA,Dubinett SM,Rome LH,Sharma S

16-7101 was used in Flow cytometry/Cell sorting to demonstrate that the vault nanocapsule can efficiently deliver CCL21 to recruit antitumour effectors, sustain antitumour activity and inhibit tumour growth.

Tue May 03 00:00:00 UTC 2011

Separation of graft-versus-host disease from graft-versus-leukemia responses by targeting CC-chemokine receptor 7 on donor T cells.

Blood

Coghill JM,Carlson MJ,Panoskaltsis-Mortari A,West ML,Burgents JE,Blazar BR,Serody JS

16-7101 was used in Flow cytometry/Cell sorting to investigate the role of MHC disparity between donor and recipient, in the attenuated graft-versus-host disease responses of murine T cells lacking CCR7.

Thu Jun 10 00:00:00 UTC 2010

Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment.

Journal of immunology (Baltimore, Md. : 1950)

Nijnik A,Madera L,Ma S,Waldbrook M,Elliott MR,Easton DM,Mayer ML,Mullaly SC,Kindrachuk J,Jenssen H,Hancock RE

16-7101 was used in an ELISA assay to study the importance of the chemokine induction activity of host defence peptides in protection against bacterial infections.

Mon Mar 01 00:00:00 UTC 2010

Role of plasmacytoid dendritic cells for aberrant class II expression in exocrine glands from estrogen-deficient mice of healthy background.

The American journal of pathology

Arakaki R,Nagaoka A,Ishimaru N,Yamada A,Yoshida S,Hayashi Y

16-7101 was used in an ELISA assay to indicate that estrogen deficiency initiates class II transactivator type IV mRNA expression in exocrine glands via epithelial and plasmacytoid DC interactions.

Fri May 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

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Gene Aliases: CSIF; Il-10; Il10

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UniProt ID: (Mouse) P18893

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Entrez Gene ID: (Mouse) 16153

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Function(s)

cytokine activity interleukin-10 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (JES5-16E3), Functional Grade, eBioscience™

IL-10 Antibody (16-7101-85) in IS

Product Details

16-7101-85

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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