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Invitrogen
IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 7
IL-12/IL-23 p40 Antibody (53-7123-82) in Flow
Mouse thioglycolate-elicited peritoneal exudate cells were stimulated overnight with LPS and IFN gamma in the presence of Brefeldin A. Cells were then fixed, permeabilized and stained with 0.125 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (left) or 0.125 µg of Anti-Mouse IL-12/IL-23 p40 Alexa Fluor® 488 (right). Total viable cells, as determined with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.
Product Details
53-7123-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
C17.8
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574437
Product Specific Information
Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.
Applications Reported: This C17.8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This C17.8 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; Interleukin12; RP23-388G23.1
Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; IL-23; Il12a; Il12b; Il12p40; Il23a; Ll12a; p19; p35; p40
UniProt ID: (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14
Entrez Gene ID: (Mouse) 16159, (Mouse) 16160, (Mouse) 83430
positive regulation of T cell mediated cytotoxicity
positive regulation of defense response to virus by host
immune system process
positive regulation of T-helper 1 type immune response
inflammatory response
immune response
positive regulation of activation of JAK2 kinase activity
negative regulation of interleukin-10 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell proliferation
T cell proliferation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat4 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of memory T cell differentiation
innate immune response
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
tissue remodeling
defense response to Gram-negative bacterium
positive regulation of NK T cell activation
positive regulation of NK T cell proliferation
defense response to virus
positive regulation of neutrophil chemotaxis
positive regulation of T-helper 17 type immune response
positive regulation of T-helper 17 cell lineage commitment
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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