Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C8.6), eBioscience™

Product Details

14-7129-85

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

View 6 publications 6 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

C8.6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_468450

Product Specific Information

Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.

Applications Reported: Unlabeled C8.6 has been reported for use as a cold mAb control to block fluorochrome conjugated C8.6 in staining of intracellular IL-12 and in immunblotting under nonreducing conditions. C8.6 has also been reported for use in cytokine neutralization. (Please use Functional Grade purified C8.6, Product # 16-7129, in functional assays).

Applications Tested: The unconjugated C8.6 antibody has been tested as a cold mAb control to block fluorochrome conjugated C8.6 in staining of intracellular IL-12 and nonreducing immunoblots at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IL23 induces IL23R recycling and amplifies innate receptor-induced signalling and cytokines in human macrophages, and the IBD-protective IL23R R381Q variant modulates these outcomes.

Gut

Sun R,Hedl M,Abraham C

14-7129-85 was used in Enzyme-linked immunosorbent assay to analyse protein and RNA expression, signalling and localisation in human monocyte-derived macrophages.

Sat Feb 01 00:00:00 UTC 2020

Regulations of Glycolytic Activities on Macrophages Functions in Tumor and Infectious Inflammation.

Frontiers in cellular and infection microbiology

Yu Q,Wang Y,Dong L,He Y,Liu R,Yang Q,Cao Y,Wang Y,Jia A,Bi Y,Liu G

14-7129-85 was used in Flow Cytometry to provide a basis for a comprehensively understanding the role of glycolysis and OXPHOS in macrophage differentiation during anti-infection and anti-tumor inflammation.

Thu Jun 17 00:00:00 UTC 2021

Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus.

Journal of immunology (Baltimore, Md. : 1950)

Tsai CY,Liong KH,Gunalan MG,Li N,Lim DS,Fisher DA,MacAry PA,Leo YS,Wong SC,Puan KJ,Wong SB

14-7129 was used in Functional assays to investigate the markers of cellular innate immunity against dengue virus, specifically how type I IFNs and IL-18 regulate activation, proliferation and degranulation of T cells against dendritic cells in human patients with dengue virus.

Wed Apr 15 00:00:00 UTC 2015

The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus.

Journal of immunology (Baltimore, Md. : 1950)

Lim DS,Yawata N,Selva KJ,Li N,Tsai CY,Yeong LH,Liong KH,Ooi EE,Chong MK,Ng ML,Leo YS,Yawata M,Wong SB

14-7129 was used in Neutralization experiments to investigate the response of NK cells against dengue-infected cells, showing that a combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells is required.

Sat Nov 15 00:00:00 UTC 2014

Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.

BioMed research international

Lopes DM,Fernandes JS,Cardoso TM,Bafica AM,Oliveira SC,Carvalho EM,Araujo MI,Cardoso LS

14-7129 was used in Flow cytometry/Cell sorting to investigate the dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.

Thu Jul 02 00:00:00 UTC 2015

Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.

PloS one

Deng N,Weaver JM,Mosmann TR

14-7129 was used in Flow cytometry/Cell sorting to study expression of IL-2 in T cell subsets during the anti-influenza response.

Thu Dec 18 00:00:00 UTC 2014

Src-kinase inhibitors sensitize human cells of myeloid origin to Toll-like-receptor-induced interleukin 12 synthesis.

Blood

Wölfl M,Schwinn S,Yoo YE,Reß ML,Braun M,Chopra M,Schreiber SC,Ayala VI,Ohlen C,Eyrich M,Beilhack A,Schlegel PG

14-7129 was used in Flow cytometry/Cell sorting to investigate the role of the dual kinase inhibitor dasatinib on human myeloid cells.

Thu Aug 15 00:00:00 UTC 2013

Immune activation and regulation in simian immunodeficiency virus-Plasmodium fragile-coinfected rhesus macaques.

Journal of virology

Trott KA,Richardson A,Hudgens MA,Abel K

14-7129 was used in Flow cytometry/Cell sorting to investigate how immune activation and elevated IL-10 interacts in HIV and malaria coinfected individuals.

Sun Sep 01 00:00:00 UTC 2013

Human monocytes promote Th1 and Th17 responses to Streptococcus pneumoniae.

Infection and immunity

Olliver M,Hiew J,Mellroth P,Henriques-Normark B,Bergman P

14-7129 was used in Neutralization experiments to examine bacterial and host factors involved in the induction of Th1 and Th17 responses.

Sat Oct 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; IL12, subunit p40; il23; IL23 p41; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; Interleukin12; JKA3 induced upon T-cell activation; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2; RP23-388G23.1

View more View less

Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL-23; IL-23A; IL12A; IL12B; IL23A; IL23P19; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P19; P35; SGRF; UNQ2498/PRO5798

View more View less

UniProt ID: (Human) P29459, (Human) P29460, (Human) Q9NPF7

View more View less

Entrez Gene ID: (Human) 3592, (Human) 3593, (Human) 51561

View more View less

Function(s)

cytokine activity interleukin-12 receptor binding protein binding growth factor activity interleukin-12 beta subunit binding interleukin-27 binding protein heterodimerization activity cytokine receptor activity interleukin-12 alpha subunit binding identical protein binding protein homodimerization activity interleukin-23 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-12/IL-23 p40 Monoclonal Antibody (C8.6), eBioscience™

Product Details

14-7129-85

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!