Invitrogen

IL-12 p70 Monoclonal Antibody (20C2)

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IL-12 p70 Antibody (M122) in ELISA

Sandwich ELISA analysis of human IL-12 was performed using a Human IL-12 (p70) colorimetric ELISA kit (Product # EHIL12) by loading 50 µL per well of a biotinylated anti-human IL-12 antibody (Product # M121B) in duplicate at 0.03125 µg/mL, and 50 µL per well of Human IL-12 (p70) Recombinant Protein (Product # SIL12) in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/mL across a 7 µg/mL anti-human IL-12 (Product # M122) pre-coated plate and incubating for 3 hours at room temperature. The plate was washed then incubated with 100 µL per well of S... View More

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Product Details

M122

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 18 publications 18 publications

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Bacteria, Human

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

20C2

Immunogen

Recombinant human IL-12 (p70)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

AB_223556

Product Specific Information

M122 targets IL-12 in ELISA, and WB applications and shows reactivity with Human samples.

The M122 immunogen is recombinant human IL-12 (p70).

M122 detects IL-12 which has a predicted molecular weight of approximately 23 kDa.

The M122 IL12 antibody (clone 20C2) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M121B (biotinylated conjugate of clone C8.6) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M122 (clone 20C2) and biotinylated antibody M121B (clone C8.6) have successfully been used in combination with recombinant IL12 p70 protein SIL12 in ELISA applications.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1.

EBioMedicine

Kristoff J,Palma ML,Garcia-Bates TM,Shen C,Sluis-Cremer N,Gupta P,Rinaldo CR,Mailliard RB

M122 was used in an ELISA assay to test the use of antigen-presenting type 1-polarized, monocyte-derived dendritic cells generated from chronic HIV-1-infected individuals on ART as a means to induce HIV-1 latency reversal in autologous CD4+ T cells harboring replication-competent provirus.

Wed May 01 00:00:00 UTC 2019

The Phosphoinositide Kinase PIKfyve Promotes Cathepsin-S-Mediated Major Histocompatibility Complex Class II Antigen Presentation.

iScience

Baranov MV,Bianchi F,Schirmacher A,van Aart MAC,Maassen S,Muntjewerff EM,Dingjan I,Ter Beest M,Verdoes M,Keyser SGL,Bertozzi CR,Diederichsen U,van den Bogaart G

M122 was used in an ELISA assay to study the role of PIKfyve in the processing and presentation of antigens.

Fri Jan 25 00:00:00 UTC 2019

A Comparative Study of the T Cell Stimulatory and Polarizing Capacity of Human Primary Blood Dendritic Cell Subsets.

Mediators of inflammation

Sittig SP,Bakdash G,Weiden J,Sköld AE,Tel J,Figdor CG,de Vries IJ,Schreibelt G

Published figure using IL-12 p70 monoclonal antibody (Product # M122) in ELISA

Tue Jan 17 00:00:00 UTC 2017

Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity.

Oncoimmunology

van Beek JJ,Gorris MA,Sköld AE,Hatipoglu I,Van Acker HH,Smits EL,de Vries IJ,Bakdash G

Published figure using IL-12 p70 monoclonal antibody (Product # M122) in ELISA

Tue Jan 12 00:00:00 UTC 2021

IL-12 receptor β1 deficiency alters in vivo T follicular helper cell response in humans.

Blood

Schmitt N,Bustamante J,Bourdery L,Bentebibel SE,Boisson-Dupuis S,Hamlin F,Tran MV,Blankenship D,Pascual V,Savino DA,Banchereau J,Casanova JL,Ueno H

M122 was used in blocking or activating experiment to study human T follicular helper cell responses in individuals lacking IL-Rbeta1

Thu Apr 25 00:00:00 UTC 2013

Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta.

Retrovirology

Dolcini GL,Solana ME,Andreani G,Celentano AM,Parodi LM,Donato AM,Elissondo N,González Cappa SM,Giavedoni LD,Martínez Peralta L

M122 was used in ELISA to study the role of Trypanosoma cruzi in HIV-1 replication in human placenta

Tue Jul 01 00:00:00 UTC 2008

Interleukin-12 production by leukemia-derived dendritic cells counteracts the inhibitory effect of leukemic microenvironment on T cells.

Experimental hematology

Curti A,Pandolfi S,Aluigi M,Isidori A,Alessandrini I,Chiodoni C,Testoni N,Colombo MP,Baccarani M,Lemoli RM

M122 was used in blocking/activating experiment to investigate the role of interleukin-12 produced by leukemia-derived dendritic cells for the inhibitory effect of leukemic microenvironment on T cells

Thu Dec 01 00:00:00 UTC 2005

High production of proinflammatory and Th1 cytokines by dendritic cells from patients with rheumatoid arthritis, and down regulation upon FcgammaR triggering.

Annals of the rheumatic diseases

Radstake TR,van Lent PL,Pesman GJ,Blom AB,Sweep FG,Rönnelid J,Adema GJ,Barrera P,van den Berg WB

M122 was used in ELISA to investigate the production of proinflammatory and Th1 cytokines by dendritic cells from patients with rheumatoid arthritis

Tue Jun 01 00:00:00 UTC 2004

Vitamin E-loaded dialyzer resets PBMC-operated cytokine network in dialysis patients.

Kidney international

Libetta C,Zucchi M,Gori E,Sepe V,Galli F,Meloni F,Milanesi F,Dal Canton A

M122 was used in ELISA to study the effect of vitamin E on cytokine profile in dialysis patients

Thu Apr 01 00:00:00 UTC 2004

Interleukin 6 and 12, alanine aminotransferase activity, and HCV viral load in children with chronic hepatitis C treated with interferon and ribavirin.

Inflammation

Kowala-Piaskowska A,Mozer-Lisewska I,Figlerowicz M,Machowska L,Słuzewski W

M122 was used in ELISA to study the effect of interferon and ribavirin treatment on interluekin 6 and 12, alanine aminotransferase activity, and HCV viral load

Wed Dec 01 00:00:00 UTC 2004

Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.

Journal of hepatology

Loffreda S,Muratori P,Muratori L,Mele L,Bianchi FB,Lenzi M

M122 was used in ELISA to investigate the augmented production of Th1-type cytokines in HCV-infected cryoglobulinemic patients

Sat Feb 01 00:00:00 UTC 2003

Altered maturation of peripheral blood dendritic cells in patients with breast cancer.

British journal of cancer

Della Bella S,Gennaro M,Vaccari M,Ferraris C,Nicola S,Riva A,Clerici M,Greco M,Villa ML

M122 was used in an ELISA assay to evaluate the in vivo relevance of two pathways in breast cancer patients.

Mon Oct 20 00:00:00 UTC 2003

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Transplantation proceedings

Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M

M122 was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients

Sun Jun 01 00:00:00 UTC 2003

Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.

Infection and immunity

McDowell MA,Marovich M,Lira R,Braun M,Sacks D

M122 was used in ELISA to investigate the mechanism for the effect of Leishmania on interleukin 12 p70 secretion

Thu Aug 01 00:00:00 UTC 2002

Monocyte induction of IL-10 and down-regulation of IL-12 by iC3b deposited in ultraviolet-exposed human skin.