Invitrogen

IL-13 Monoclonal Antibody (eBio1316H), Biotin, eBioscience™

10 Published Figures

15 References

View all (67) IL-13 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (10)

IL-13 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

IL-13 Antibody (13-7135-81) in ICC/IF

JCI insight 2020 - Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Immunocytochemistry

IL-13 Antibody in Immunohistochemistry (IHC)

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Product Details

13-7135-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 3 publications 3 publications

ELISA (ELISA)

0.25-1 µg/mL

View 6 publications 6 publications

Neutralization (Neu)

View 3 publications 3 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio1316H

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_763555

Product Specific Information

Description: The eBio1316H antibody reacts with mouse IL-13. The eBio1316H antibody is a neutralizing antibody. Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Ralpha chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.

Applications Reported: The eBio1316H antibody has been reported useful for neutralization, ELISA detection, and ELISPOT detection.

Applications Tested: The biotinylated eBio1316H antibody has been tested as the detection antibody in a sandwich ELISA (and ELISPOT assay) for analysis of mouse interleukin-13 (IL-13) in combination with the purified eBio13A (14-7133) antibody for capture and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25-1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

For intracellular staining of mouse IL-13, the fluorochrome labelled eBio13A antibody is recommended.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An innate IL-25-ILC2-MDSC axis creates a cancer-permissive microenvironment for Apc mutation-driven intestinal tumorigenesis.

Science immunology

Jou E,Rodriguez-Rodriguez N,Ferreira AF,Jolin HE,Clark PA,Sawmynaden K,Ko M,Murphy JE,Mannion J,Ward C,Matthews DJ,Buczacki SJA,McKenzie ANJ

Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Neutralization

Fri Jun 03 00:00:00 UTC 2022

IL-13 is a driver of COVID-19 severity.

JCI insight

Donlan AN,Sutherland TE,Marie C,Preissner S,Bradley BT,Carpenter RM,Sturek JM,Ma JZ,Moreau GB,Donowitz JR,Buck GA,Serrano MG,Burgess SL,Abhyankar MM,Mura C,Bourne PE,Preissner R,Young MK,Lyons GR,Loomba JJ,Ratcliffe SJ,Poulter MD,Mathers AJ,Day AJ,Mann BJ,Allen JE,Petri WA

Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Neutralization

Mon Aug 09 00:00:00 UTC 2021

IL-13 is a driver of COVID-19 severity.

medRxiv : the preprint server for health sciences

Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Neutralization

Mon Mar 01 00:00:00 UTC 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

13-7135-81 was used in Flow cytometry/Cell sorting to demonstrate a critical role for antigen-presenting ILC3s in orchestrating immune tolerance in the airway by restricting pro-inflammatory T cell responses to both allergens and microbes.

Tue Nov 23 00:00:00 UTC 2021

BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice.

JCI insight

Radhakrishnan RK,Thandi RS,Tripathi D,Paidipally P,McAllister MK,Mulik S,Samten B,Vankayalapati R

Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Immunocytochemistry

Thu Mar 12 00:00:00 UTC 2020

T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.

Cell reports

Yan J,Pandey SP,Barnes BJ,Turner JR,Abraham C

13-7135 was used in an ELISA assay to identify a previously undefined key role for T cell-intrinsic IRF5. In mice, IRF5 in CD4+ T cells promotes Th1- and Th17-associated cytokines and decreases Th2-associated cytokines.

Tue Jun 30 00:00:00 UTC 2020

Allergen-specific IgG antibody signaling through FcγRIIb promotes food tolerance.

The Journal of allergy and clinical immunology

Burton OT,Tamayo JM,Stranks AJ,Koleoglou KJ,Oettgen HC

13-7135 was used in an ELISA assay to suggest that allergen-specific IgG antibodies can act to induce and sustain immunologic tolerance to foods.

Mon Jan 01 00:00:00 UTC 2018

IgE promotes type 2 innate lymphoid cells in murine food allergy.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Burton OT,Medina Tamayo J,Stranks AJ,Miller S,Koleoglou KJ,Weinberg EO,Oettgen HC

13-7135 was used in an ELISA assay to determine the effect of IgE mediated mast cell activation on intestinal ILC3 responses following ingestion of food allergens.

Thu Mar 01 00:00:00 UTC 2018

T cell-intrinsic IL-1R signaling licenses effector cytokine production by memory CD4 T cells.

Nature communications

Jain A,Song R,Wakeland EK,Pasare C

13-7135 was used in Flow cytometry/Cell sorting to study the role of IL-1 as a licensing signal for memory CD4 T cell function.

Thu Aug 09 00:00:00 UTC 2018

Intratracheal myriocin enhances allergen-induced Th2 inflammation and airway hyper-responsiveness.

Immunity, inflammation and disease

Edukulla R,Rehn KL,Liu B,McAlees JW,Hershey GK,Wang YH,Lewkowich I,Lindsley AW

13-7135 was used in an ELISA assay to evaluate the immune regulatory role of de novo ceramide generation in vitro and in vivo.

Thu Sep 01 00:00:00 UTC 2016

Group 2 innate lymphoid cells facilitate sensitization to local, but not systemic, TH2-inducing allergen exposures.

The Journal of allergy and clinical immunology

Gold MJ,Antignano F,Halim TY,Hirota JA,Blanchet MR,Zaph C,Takei F,McNagny KM

13-7135 was used in an ELISA assay to reveal a critical role for ILC2s in the development of adaptive type 2 responses to local antigen exposure.

Tue Apr 01 00:00:00 UTC 2014

Signaling pathways activated by a protease allergen in basophils.

Proceedings of the National Academy of Sciences of the United States of America

Rosenstein RK,Bezbradica JS,Yu S,Medzhitov R

13-7135 was used in Flow cytometry/Cell sorting to study signaling pathways activated by a protease allergen in basophils.

Tue Nov 18 00:00:00 UTC 2014

Interleukin 13- and interleukin 17A-induced pulmonary hypertension phenotype due to inhalation of antigen and fine particles from air pollution.

Pulmonary circulation

Park SH,Chen WC,Esmaeil N,Lucas B,Marsh LM,Reibman J,Grunig G

Mon Dec 01 00:00:00 UTC 2014

Contribution of IL-13 to early exocrinopathy in Id3-/- mice.

Molecular immunology

Mahlios J,Zhuang Y

13-7135 was used in Immunofluorescence to examine the role of cytokines produced by T cells in the systemic regulation of salivary gland function.

Sat Oct 01 00:00:00 UTC 2011

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

13-7135 was used in an ELISA assay to suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.

Wed Jul 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

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Gene Aliases: Il-13; Il13

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UniProt ID: (Mouse) P20109

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Entrez Gene ID: (Mouse) 16163

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Function(s)

cytokine activity cytokine receptor binding interleukin-13 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-13 Monoclonal Antibody (eBio1316H), Biotin, eBioscience™

IL-13 Antibody (13-7135-81) in ICC/IF

Product Details

13-7135-81

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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