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IL-15 Antibody (500-P173BT-25UG) in WB
Western Blot: To detect mIL-15 by Western Blot analysis IL-15 Polyclonal Antibody, Biotin (Product # 500-P173BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-15 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P173BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-15
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929633
Product Specific Information
AA Sequence of recombinant protein: MNWIDVRYDL EKIESLIQSI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VAESGCKECE ELEEKTFTEF LQSFIRIVQM FINTS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-15. Anti-Murine IL-15-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mIL-15 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-15 (500-P173) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-15.
Western Blot: To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-15 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P173BT-1MG will be provided as 2 x 500 µg
Target Information
IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: il 15; IL-15; ILN; Interleukin; Interleukin-15; Interleukin15; M-IL-15; MGC9721
Gene Aliases: AI503618; IL-15; Il15
UniProt ID: (Mouse) P48346
Entrez Gene ID: (Mouse) 16168
natural killer cell differentiation
NK T cell proliferation
immune response
signal transduction
positive regulation of cell proliferation
hyaluronan metabolic process
positive regulation of interleukin-17 production
positive regulation of natural killer cell proliferation
positive regulation of natural killer cell differentiation
positive regulation of T cell proliferation
tyrosine phosphorylation of Stat5 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
extrathymic T cell selection
regulation of T cell differentiation
cell maturation
lymph node development
negative regulation of smooth muscle cell proliferation
regulation of defense response to virus by host
positive regulation of immune response
positive regulation of protein O-linked glycosylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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