Invitrogen

IL-17A Monoclonal Antibody (eBioMM17F3), Functional Grade, eBioscience™

5 Published Figures

23 References

View all (159) IL-17A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (5)

IL-17A Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 5

IL-17A Antibody (16-7173-81) in IHC

Proceedings of the National Academy of Sciences of the United States of America 2014 - Published figure using IL-17A monoclonal antibody (Product # 16-7173-81) in Immunohistochemistry

IL-17A Antibody in Flow Cytometry (Flow)

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Product Details

16-7173-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

View 12 publications 12 publications

Functional Assay (Functional)

Assay-Dependent

View 6 publications 6 publications

Inhibition Assays (IA)

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioMM17F3

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_763583

Product Specific Information

Description: The eBioMM17F3 antibody is a mouse IgG1 immunoglobulin which reacts with mouse IL-17A. The eBioMM17F3 antibody is a neutralizing antibody. The eBioMM17F3 antibody has been tested by indirect ELISA against mouse IL-17B, IL-17C, IL-17D, IL-17E, and IL-17F, and has been found not to react to these other IL-17 family members. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Applications Reported:The eBioMM17F3 antibody has been reported useful for neutralization of mouse IL-17A bioactivity and for intracellular staining of IL-17A-producing cells (IC Flow).

Applications Tested: The Functional Grade Purified eBioMM17F3 antibody has been tested by LAL assay to verify low endotoxin level and has been tested for neutralization of IL-17A bioactivity.

For in vitro neutralization, the Functional Grade purified eBioMM17F3 antibody at 8.0 ng/mL has been found to neutralize by 50% the biological effect of 5.0 ng/mL mouse IL-17A in an assay of IL-6 secretion by NIH/3T3 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation:Less than 10%, as determined by HPLC.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Xuanfei Baidu Decoction reduces acute lung injury by regulating infiltration of neutrophils and macrophages via PD-1/IL17A pathway.

Pharmacological research

Wang Y,Wang X,Li Y,Xue Z,Shao R,Li L,Zhu Y,Zhang H,Yang J

Published figure using IL-17A monoclonal antibody (Product # 16-7173-81) in Neutralization

Tue Feb 01 00:00:00 UTC 2022

RKIP mediates autoimmune inflammation by positively regulating IL-17R signaling.

EMBO reports

Lin W,Wang N,Zhou K,Su F,Jiang Y,Shou J,Liu H,Ma C,Qian Y,Wang K,Wang X

Published figure using IL-17A monoclonal antibody (Product # 16-7173-81) in Neutralization

Fri Jun 01 00:00:00 UTC 2018

NDR1 protein kinase promotes IL-17- and TNF-α-mediated inflammation by competitively binding TRAF3.

EMBO reports

Ma C,Lin W,Liu Z,Tang W,Gautam R,Li H,Qian Y,Huang H,Wang X

16-7173 was used in Neutralization experiments to study how IL-17 signal transduction is regulated, and the importance of this in numerous autoimmune disorders.

Sat Apr 01 00:00:00 UTC 2017

Protective efficacy of the chimeric Staphylococcus aureus vaccine candidate IC in sepsis and pneumonia models.

Scientific reports

Yang L,Cai C,Feng Q,Shi Y,Zuo Q,Yang H,Jing H,Wei C,Zhuang Y,Zou Q,Zeng H

16-7173 was used in Neutralization experiments to design a novel chimeric vaccine for S. aureus infection.

Thu Feb 11 00:00:00 UTC 2016

Protective efficacy of the chimeric Staphylococcus aureus vaccine candidate IC in sepsis and pneumonia models.

Scientific reports

Yang L,Cai C,Feng Q,Shi Y,Zuo Q,Yang H,Jing H,Wei C,Zhuang Y,Zou Q,Zeng H

16-7173 was used in Neutralization experiments to design a novel chimeric vaccine for S. aureus infection.

Thu Feb 11 00:00:00 UTC 2016

Th17-biased RORγt transgenic mice become susceptible to a viral model for multiple sclerosis.

Brain, behavior, and immunity

Martinez NE,Sato F,Kawai E,Omura S,Takahashi S,Yoh K,Tsunoda I

16-7173 was used in Neutralization experiments to suggest that a Th17-biased "gain-of-function" mutation could increase susceptibility to virus-mediated demyelinating diseases.

Thu Jan 01 00:00:00 UTC 2015

Th17-biased RORγt transgenic mice become susceptible to a viral model for multiple sclerosis.

Brain, behavior, and immunity

Martinez NE,Sato F,Kawai E,Omura S,Takahashi S,Yoh K,Tsunoda I

16-7173 was used in Neutralization experiments to suggest that a Th17-biased "gain-of-function" mutation could increase susceptibility to virus-mediated demyelinating diseases.

Thu Jan 01 00:00:00 UTC 2015

Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Li JT,Melton AC,Su G,Hamm DE,LaFemina M,Howard J,Fang X,Bhat S,Huynh KM,O'Kane CM,Ingram RJ,Muir RR,McAuley DF,Matthay MA,Sheppard D

16-7173 was used in Blocking experiments to study the role of adaptive immune cells in acute respiratory distress syndrome (ARDS), suggesting that IL-17A released by αβTh17 cells plays a significant role and could be a target for therapy.

Wed Jul 01 00:00:00 UTC 2015

Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Li JT,Melton AC,Su G,Hamm DE,LaFemina M,Howard J,Fang X,Bhat S,Huynh KM,O'Kane CM,Ingram RJ,Muir RR,McAuley DF,Matthay MA,Sheppard D

Wed Jul 01 00:00:00 UTC 2015

IL-17A is a novel player in dialysis-induced peritoneal damage.

Kidney international

Rodrigues-Díez R,Aroeira LS,Orejudo M,Bajo MA,Heffernan JJ,Rodrigues-Díez RR,Rayego-Mateos S,Ortiz A,Gonzalez-Mateo G,López-Cabrera M,Selgas R,Egido J,Ruiz-Ortega M

16-7173 was used in Neutralization experiments to identify IL-17A as a key mediator of peritoneal inflammation and fibrosis in dialysis patients.

Fri Aug 01 00:00:00 UTC 2014

IL-17A is a novel player in dialysis-induced peritoneal damage.

Kidney international

Rodrigues-Díez R,Aroeira LS,Orejudo M,Bajo MA,Heffernan JJ,Rodrigues-Díez RR,Rayego-Mateos S,Ortiz A,Gonzalez-Mateo G,López-Cabrera M,Selgas R,Egido J,Ruiz-Ortega M

16-7173 was used in Neutralization experiments to identify IL-17A as a key mediator of peritoneal inflammation and fibrosis in dialysis patients.

Fri Aug 01 00:00:00 UTC 2014

Mechanisms of NDV-3 vaccine efficacy in MRSA skin versus invasive infection.

Proceedings of the National Academy of Sciences of the United States of America

Yeaman MR,Filler SG,Chaili S,Barr K,Wang H,Kupferwasser D,Hennessey JP,Fu Y,Schmidt CS,Edwards JE,Xiong YQ,Ibrahim AS

16-7173 was used in Neutralization experiments to evaluate the efficacy and immunologic mechanisms of a vaccine containing a recombinant glycoprotein antigen in mouse skin and skin structure infection.

Tue Dec 23 00:00:00 UTC 2014

Mechanisms of NDV-3 vaccine efficacy in MRSA skin versus invasive infection.

Proceedings of the National Academy of Sciences of the United States of America

Yeaman MR,Filler SG,Chaili S,Barr K,Wang H,Kupferwasser D,Hennessey JP,Fu Y,Schmidt CS,Edwards JE,Xiong YQ,Ibrahim AS

Tue Dec 23 00:00:00 UTC 2014

Interleukin-6 (IL-6) and IL-17 synergistically promote viral persistence by inhibiting cellular apoptosis and cytotoxic T cell function.

Journal of virology

Hou W,Jin YH,Kang HS,Kim BS

16-7173 was used in Flow cytometry/Cell sorting to indicate that an excessive level of IL-6 cytokine produced following viral infection promotes the development of IL-17-producing pathogenic helper T cells.

Fri Aug 01 00:00:00 UTC 2014

Th17 can regulate silica-induced lung inflammation through an IL-1β-dependent mechanism.

Journal of cellular and molecular medicine

Song L,Weng D,Dai W,Tang W,Chen S,Li C,Chen Y,Liu F,Chen J

16-7173 was used in Blocking experiments to understand the underlying mechanisms of IL-1β and IL-17 in silicosis, which may aid development of novel therapies for the disease.

Mon Sep 01 00:00:00 UTC 2014

Reduction of IL-17A might suppress the Th1 response and promote the Th2 response by boosting the function of Treg cells during silica-induced inflammatory response in vitro.

Mediators of inflammation

Tang W,Liu F,Chen Y,Song L,Dai W,Li C,Weng D,Chen J

16-7173 was used in Functional assays to study the role of IL-17A in the silica-induced inflammatory response.

Mon Dec 15 00:00:00 UTC 2014

T-bet regulates immunity to Francisella tularensis live vaccine strain infection, particularly in lungs.

Infection and immunity

Melillo AA,Foreman O,Bosio CM,Elkins KL

16-7173 was used in an ELISA assay to demonstrate that T-bet is important in host resistance to primary live vaccine strain infection and intranasal secondary challenge.

Tue Apr 01 00:00:00 UTC 2014

Induced IL-17-producing invariant NKT cells require activation in presence of TGF-β and IL-1β.

Journal of immunology (Baltimore, Md. : 1950)

Monteiro M,Almeida CF,Agua-Doce A,Graca L

16-7173 was used in Neutralization experiments to demonstrate that IL-17 production by iNKT cells can be imprinted in natural iNKT17 cells or peripherally induced.

Tue Jan 15 00:00:00 UTC 2013

Interleukin-10 (IL-10) inhibits Borrelia burgdorferi-induced IL-17 production and attenuates IL-17-mediated Lyme arthritis.

Infection and immunity

Hansen ES,Medić V,Kuo J,Warner TF,Schell RF,Nardelli DT

16-7173 was used in Neutralization experiments to study the roles of interleukin (IL)-10 and IL-17 in the development of Lyme arthritis.

Sun Dec 01 00:00:00 UTC 2013

Inhibition of CD73 improves B cell-mediated anti-tumor immunity in a mouse model of melanoma.

Journal of immunology (Baltimore, Md. : 1950)

Forte G,Sorrentino R,Montinaro A,Luciano A,Adcock IM,Maiolino P,Arra C,Cicala C,Pinto A,Morello S

16-7173 was used in Neutralization experiments to demonstrate that both T-cells and B cells play a pivotal role in the APCP-induced anti-tumor immune response.

Sat Sep 01 00:00:00 UTC 2012

Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation.

The Journal of allergy and clinical immunology

Liu SY,Wang WZ,Yen CL,Tsai MY,Yang PW,Wang JY,Ho CY,Shieh CC

16-7173 was used in an ELISA assay to reveal that leukocyte NADPH oxidase is an essential regulator of toluene diisocyanate-induced airway inflammation through redox modification of immune responses.

Fri Apr 01 00:00:00 UTC 2011

Molecular immune responses to aerosol challenge with Francisella tularensis in mice inoculated with live vaccine candidates of varying efficacy.

PloS one

Shen H,Harris G,Chen W,Sjostedt A,Ryden P,Conlan W

16-7173 was used in Immunohistochemistry to characterize local and systemic cytokine and chemokine responses in mice immunized with vaccines with different efficacies against aerosol challenge.

Tue Oct 12 00:00:00 UTC 2010

In vitro-differentiated TH17 cells mediate lethal acute graft-versus-host disease with severe cutaneous and pulmonary pathologic manifestations.

Blood

Carlson MJ,West ML,Coghill JM,Panoskaltsis-Mortari A,Blazar BR,Serody JS

16-7173-81 was used in In vitro Assay to demonstrate that a highly purified population of Th17 cells is capable of inducing lethal graft-versus-host disease, hallmarked by extensive pathologic cutaneous and pulmonary lesions.

Thu Feb 05 00:00:00 UTC 2009

Th17 cells enhance viral persistence and inhibit T cell cytotoxicity in a model of chronic virus infection.

The Journal of experimental medicine

Hou W,Kang HS,Kim BS

Mon Feb 16 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; il 17; IL-17; ILN; Interleukin; interleukin 17; Interleukin-17A; Interleukin17A; R-IL-17-A

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Gene Aliases: Ctla-8; Ctla8; IL-17; IL-17A; Il17; Il17a

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UniProt ID: (Mouse) Q62386

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Entrez Gene ID: (Mouse) 16171

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Function(s)

cytokine activity

Process(es)

inflammatory response positive regulation of necrotic cell death positive regulation of interleukin-23 production positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter cellular response to interleukin-1 cellular response to glucocorticoid stimulus fibroblast activation positive regulation of cytokine production involved in inflammatory response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-17A Monoclonal Antibody (eBioMM17F3), Functional Grade, eBioscience™

IL-17A Antibody (16-7173-81) in IHC

Product Details

16-7173-81

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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