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Invitrogen
IL-17F Monoclonal Antibody (H17F10A7), eBioscience™
FIGURE: 1 / 1
IL-17F Antibody (14-7479-82) in WB
IL-17 producing cells were generated by culturing human PBMC with anti-Human CD3 (Product # 16-0039-81) and anti-Human CD28 (Product # 16-0289-81) in the presence of Human IL-23 Recombinant for 3 days, followed by 3 days with IL-23 alone. Day 6 cultures were treated with Brefeldin A alone (1) or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours. Cell lysates were run under reducing conditions, immunoblotted with 2 µg/mL of Anti-Human IL-17F Purified and revealed with Anti-Mouse HRP.
Product Details
14-7479-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
H17F10A7
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_891574
Product Specific Information
Description: The monoclonal antibody H17F10A7 reacts with human IL-17F, a member of the IL-17 family of pro-inflammatory cytokines. Like IL-17A, IL-17F is a disulfide-linked, homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-β, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-β and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). These heterodimers can be detected by immunoprecipitation with eBio64CAP17 anti-IL-17A monoclonal antibody followed by immunoblot with H17F10A7.
Applications Reported: This H17F10A7 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This H17F10A7 antibody has been tested at 1-2µg/mL by western blot analysis of cultured, activated Th17 cells, where it detects a band of approximately 18kDa.
It is recommended that H17F10A7 be used under reducing conditions only.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-17F (Interleukin 17F, CTLA-8)) is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils. IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. IL-17F may be secreted as a homodimer, or a heterodimer with IL17A. It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflammation by increasing chemokine production. IL-17F also stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17F is produced, and exists, as a homo-dimer, with homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells. IL-17F has been found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytokine ML-1; IL-17F; IL-24; ILN; Interleukin; Interleukin-17F; interleukin-24; Interleukin17F; mutant IL-17F
Gene Aliases: CANDF6; IL-17F; IL17F; ML-1; ML1
UniProt ID: (Human) Q96PD4
Entrez Gene ID: (Human) 112744
inflammatory response
negative regulation of angiogenesis
regulation of transforming growth factor beta receptor signaling pathway
cytokine biosynthetic process
lymphotoxin A biosynthetic process
regulation of interleukin-2 biosynthetic process
regulation of interleukin-6 biosynthetic process
regulation of interleukin-8 biosynthetic process
regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
cartilage development
positive regulation of cytokine production involved in inflammatory response
positive regulation of interleukin-6 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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