Rockland

IL-18 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

210-401-323

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:200
-

ELISA (ELISA)

1:1,000-1:5,000
-

Immunoprecipitation (IP)

1:500-1:2,000
-

Neutralization (Neu)

1:50-1:400
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions. A control of similarly diluted LOW ENDOTOXIN CONTROL RABBIT IgG (code # 011-001-297) is recommended.

Target Information

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; Interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320

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Gene Aliases: Igif; Il-18; Il18

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UniProt ID: (Mouse) P70380

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Entrez Gene ID: (Mouse) 16173

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Function(s)
cytokine activity
Process(es)
MAPK cascade angiogenesis response to hypoxia inflammatory response immune response extrinsic apoptotic signaling pathway via death domain receptors positive regulation of gene expression positive regulation of smooth muscle cell migration natural killer cell activation regulation of cell adhesion sleep lipopolysaccharide-mediated signaling pathway positive regulation of chemokine production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-17 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell proliferation positive regulation of superoxide anion generation positive regulation of collagen biosynthetic process negative regulation of neutrophil apoptotic process T-helper 1 type immune response interferon-gamma biosynthetic process positive regulation of activated T cell proliferation interleukin-13 biosynthetic process positive regulation of NF-kappaB import into nucleus positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of apoptotic process positive regulation of vascular permeability positive regulation of neuron apoptotic process negative regulation of myoblast differentiation positive regulation of smooth muscle cell proliferation negative regulation of peptidyl-tyrosine phosphorylation detection of mechanical stimulus involved in sensory perception of pain positive regulation of NK T cell proliferation cellular response to lipopolysaccharide cellular response to peptide hormone stimulus cellular response to organic cyclic compound positive regulation of male germ cell proliferation positive regulation of blood vessel remodeling
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