Invitrogen

IL-2 Monoclonal Antibody (JES6-5H4), Alexa Fluor™ 488, eBioscience™

FIGURE: 1 / 26

IL-2 Antibody (53-7021-80) in Flow

Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 APC and buffer (left) or Anti-Mouse IL-2 Alexa Fluor® 488 (right).

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

53-7021-80

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 19 publications 19 publications

ELISA (ELISA)

View 4 publications 4 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES6-5H4

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_469919

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: The JES6-5H4 antibody has been reported useful for ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: The Alexa Fluor® 488 JES6-5H4 antibody has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

Yang G,Wang J,Wang S,Zhu Y

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Sat Aug 20 00:00:00 UTC 2022

Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

Zou Y,Wang X,Xu J,Wang S,Li S,Zhu Y,Wang J

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Mon Oct 31 00:00:00 UTC 2022

In vivo genome-wide CRISPR screening identifies ZNF24 as a negative NF-κB modulator in lung cancer.

Cell & bioscience

Liu L,Lei Y,Chen W,Zhou Q,Zheng Z,Zeng G,Liu W,Feng P,Zhang Z,Yu L,Chen L

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2022

Impaired Treg-DC interactions contribute to autoimmunity in leukocyte adhesion deficiency type 1.

JCI insight

Klaus T,Wilson AS,Vicari E,Hadaschik E,Klein M,Helbich SSC,Kamenjarin N,Hodapp K,Schunke J,Haist M,Butsch F,Probst HC,Enk AH,Mahnke K,Waisman A,Bednarczyk M,Bros M,Bopp T,Grabbe S

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Thu Dec 22 00:00:00 UTC 2022

A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters.

Journal of virology

Lu M,Zhang Y,Dravid P,Li A,Zeng C,Kc M,Trivedi S,Sharma H,Chaiwatpongsakorn S,Zani A,Kenney A,Cai C,Ye C,Liang X,Qiu J,Martinez-Sobrido L,Yount JS,Boyaka PN,Liu SL,Peeples ME,Kapoor A,Li J

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Mon Sep 27 00:00:00 UTC 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Mon May 10 00:00:00 UTC 2021

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Mon Jun 08 00:00:00 UTC 2020

Deletion Timing of Cic Alleles during Hematopoiesis Determines the Degree of Peripheral CD4⁺ T Cell Activation and Proliferation.

Immune network

Park GY,Lee GW,Kim S,Hong H,Park JS,Cho JH,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in ELISA

Thu Oct 01 00:00:00 UTC 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Thu Nov 05 00:00:00 UTC 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Fri Jan 10 00:00:00 UTC 2020

miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival.

Cell reports

Gagnon JD,Kageyama R,Shehata HM,Fassett MS,Mar DJ,Wigton EJ,Johansson K,Litterman AJ,Odorizzi P,Simeonov D,Laidlaw BJ,Panduro M,Patel S,Jeker LT,Feeney ME,McManus MT,Marson A,Matloubian M,Sanjabi S,Ansel KM

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Tue Aug 20 00:00:00 UTC 2019

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Wed Nov 20 00:00:00 UTC 2019

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Wed Oct 11 00:00:00 UTC 2017

Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.

Nature communications

Park S,Lee S,Lee CG,Park GY,Hong H,Lee JS,Kim YM,Lee SB,Hwang D,Choi YS,Fryer JD,Im SH,Lee SW,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in ELISA

Wed Jul 12 00:00:00 UTC 2017

Protein Translation Activity: A New Measure of Host Immune Cell Activation.

Journal of immunology (Baltimore, Md. : 1950)

Seedhom MO,Hickman HD,Wei J,David A,Yewdell JW

53-7021 was used in Flow cytometry/Cell sorting to describe the in vivo ribopuromycylation method.

Mon Aug 15 00:00:00 UTC 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Fri Mar 11 00:00:00 UTC 2016

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in Flow Cytometry

Thu May 01 00:00:00 UTC 2014

Prevention or early cure of type 1 diabetes by intranasal administration of gliadin in NOD mice.

PloS one

Funda DP,Fundova P,Hansen AK,Buschard K

53-7021 was used in Flow cytometry/Cell sorting to investigate whether intranasal (i.n.) administration of gliadin or gluten may arrest the diabetogenic process, showing that i.n. administration of gliadin prevents type 1 diabetes in NOD mice.

Tue Jan 13 00:00:00 UTC 2015

Modulation of pulmonary dendritic cell function during mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Liu Y,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in ELISA

Fri Feb 01 00:00:00 UTC 2008

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 53-7021-82) in ELISA

Tue May 01 00:00:00 UTC 2007

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-2 Monoclonal Antibody (JES6-5H4), Alexa Fluor™ 488, eBioscience™

IL-2 Antibody (53-7021-80) in Flow

Product Details

53-7021-80

Flow Cytometry (Flow)

ELISA (ELISA)

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Performance Guarantee

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