Invitrogen

IL-2 Monoclonal Antibody (MQ1-17H12), APC, eBioscience™

FIGURE: 1 / 15

IL-2 Antibody (17-7029-82) in Flow

Human naïve CD4 T cells were stimulated with anti-CD3/CD28 beads (1:4) (Product # 11132D) for 6 days. Cells were then stimulated with PMA (50ng/ml), ionomycin (500ng/ml) and BFA (5µg/ml) for 5h before staining with antibody against IL-21 and IL-2 (Product # 17-7029-82) at the concentration of 1:100 . Data courtesy of the antibody data exchange program.

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IL-2 clone MQ1-17H12 stains a subset of CD4+ T cells only after stimulation (top) and does not stain CD19+ B cells regardless of stimulation (top and bottom). Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IL-2 (clone MQ1-17H12). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

17-7029-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ1-17H12

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_469492

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported:MQ1-17H12 has been reported for use in staining of intracellular IL-2.

Applications Tested: Has been tested intracellular staining and flow cytometric analysis of activated human normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T cell lymphoma treatment.

Molecular therapy oncolytics

Xia B,Lin K,Wang X,Chen F,Zhou M,Li Y,Lin Y,Qiao Y,Li R,Zhang W,He X,Zou F,Li L,Lu L,Chen C,Li W,Zhang H,Liu B

17-7029-82 was used in Flow cytometry/Cell sorting to show that, because Nbs are smaller and less immunogenic, the synergistic effect of Nb-based bispecific CAR-T cells may improve their safety and efficacy in future clinical applications.

Thu Sep 21 00:00:00 UTC 2023

Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T cell lymphoma treatment.

Molecular therapy oncolytics

Xia B,Lin K,Wang X,Chen F,Zhou M,Li Y,Lin Y,Qiao Y,Li R,Zhang W,He X,Zou F,Li L,Lu L,Chen C,Li W,Zhang H,Liu B

Thu Sep 21 00:00:00 UTC 2023

Baseline innate and T cell populations are correlates of protection against symptomatic influenza virus infection independent of serology.

Nature immunology

Mettelman RC,Souquette A,Van de Velde LA,Vegesana K,Allen EK,Kackos CM,Trifkovic S,DeBeauchamp J,Wilson TL,St James DG,Menon SS,Wood T,Jelley L,Webby RJ,Huang QS,Thomas PG

17-7029-82 was used in Flow cytometry/Cell sorting to demonstrate that preinfection peripheral cell composition improves the prediction of symptomatic influenza susceptibility over vaccination, demographics or serology alone.

Fri Sep 01 00:00:00 UTC 2023

SARS-CoV-2 mRNA vaccination elicits a robust and persistent T follicular helper cell response in humans.

Cell

Mudd PA,Minervina AA,Pogorelyy MV,Turner JS,Kim W,Kalaidina E,Petersen J,Schmitz AJ,Lei T,Haile A,Kirk AM,Mettelman RC,Crawford JC,Nguyen THO,Rowntree LC,Rosati E,Richards KA,Sant AJ,Klebert MK,Suessen T,Middleton WD,Wolf J,Teefey SA,O'Halloran JA,Presti RM,Kedzierska K,Rossjohn J,Thomas PG,Ellebedy AH

17-7029-82 was used in Flow Cytometry to evaluate the T cell receptor sequences and phenotype of SARS-CoV-2 vaccine-induced lymph node follicular helper CD4+ T cells.

Thu Feb 17 00:00:00 UTC 2022

Dynamics of spike-and nucleocapsid specific immunity during long-term follow-up and vaccination of SARS-CoV-2 convalescents.

Nature communications

Koerber N,Priller A,Yazici S,Bauer T,Cheng CC,Mijočević H,Wintersteller H,Jeske S,Vogel E,Feuerherd M,Tinnefeld K,Winter C,Ruland J,Gerhard M,Haller B,Christa C,Zelger O,Roggendorf H,Halle M,Erber J,Lingor P,Keppler O,Zehn D,Protzer U,Knolle PA

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Mon Jan 10 00:00:00 UTC 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Tue Oct 18 00:00:00 UTC 2022

Dissecting the role of CSF2RB expression in human regulatory T cells.

Frontiers in immunology

Côrte-Real BF,Arroyo Hornero R,Dyczko A,Hamad I,Kleinewietfeld M

17-7029-82 was used in Flow Cytometry to identify Treg subpopulations from pools of activated CD4+ T cells and demonstrate the overexpression of CSF2RB in Tregs of patients with autoimmune diseases like MS and SLE, thereby indicating that CSF2RB expression in Tregs could serve as a potential novel biomarker for disease.

Tue Dec 20 00:00:00 UTC 2022

Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.

Frontiers in cellular and infection microbiology

Kathamuthu GR,Kumar NP,Moideen K,Menon PA,Babu S

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Thu Nov 25 00:00:00 UTC 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Tue Aug 24 00:00:00 UTC 2021

Platelets stimulate programmed death-ligand 1 expression by cancer cells: Inhibition by anti-platelet drugs.

Journal of thrombosis and haemostasis : JTH

Asgari A,Lesyk G,Poitras E,Govindasamy N,Terry K,To R,Back V,Rudzinski JK,Lewis JD,Jurasz P

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Mon Nov 01 00:00:00 UTC 2021

Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV.

Nature communications

Alrubayyi A,Gea-Mallorquí E,Touizer E,Hameiri-Bowen D,Kopycinski J,Charlton B,Fisher-Pearson N,Muir L,Rosa A,Roustan C,Earl C,Cherepanov P,Pellegrino P,Waters L,Burns F,Kinloch S,Dong T,Dorrell L,Rowland-Jones S,McCoy LE,Peppa D

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Tue Oct 05 00:00:00 UTC 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Fri Oct 01 00:00:00 UTC 2021

CRISPR-Mediated Base Conversion Allows Discriminatory Depletion of Endogenous T Cell Receptors for Enhanced Synthetic Immunity.

Molecular therapy. Methods & clinical development

Preece R,Pavesi A,Gkazi SA,Stegmann KA,Georgiadis C,Tan ZM,Aw JYJ,Maini MK,Bertoletti A,Qasim W

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Fri Dec 11 00:00:00 UTC 2020

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Mon Apr 02 00:00:00 UTC 2018

Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy.

Journal of immunology (Baltimore, Md. : 1950)

Wang C,Edilova MI,Wagar LE,Mujib S,Singer M,Bernard NF,Croughs T,Lederman MM,Sereti I,Fischl MA,Kremmer E,Ostrowski M,Routy JP,Watts TH

17-7029 was used in Flow cytometry/Cell sorting to show that IL-7 therapy in humans increases the number of circulating HIV-specific CD8 T cells.

Mon Jan 15 00:00:00 UTC 2018

A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.

Protein engineering, design & selection : PEDS

De Luca R,Kachel P,Kropivsek K,Snijder B,Manz MG,Neri D

Published figure using IL-2 monoclonal antibody (Product # 17-7029-82) in Flow Cytometry

Tue May 01 00:00:00 UTC 2018

Cross-Reactivity and Anti-viral Function of Dengue Capsid and NS3-Specific Memory T Cells Toward Zika Virus.

Frontiers in immunology

Lim MQ,Kumaran EAP,Tan HC,Lye DC,Leo YS,Ooi EE,MacAry PA,Bertoletti A,Rivino L

17-7029 was used in Flow Cytometry to assess the cross-reactivity and anti-viral function of Dengue capsid and NS3-specific memory T cells toward Zika virus.

Mon Oct 21 00:00:00 UTC 2019

A High Frequency of HIV-Specific Circulating Follicular Helper T Cells Is Associated with Preserved Memory B Cell Responses in HIV Controllers.

mBio

Claireaux M,Galperin M,Benati D,Nouël A,Mukhopadhyay M,Klingler J,de Truchis P,Zucman D,Hendou S,Boufassa F,Moog C,Lambotte O,Chakrabarti LA

17-7029 was used in Flow cytometry/Cell sorting to suggest that T follicular help may contribute to HIV control and highlight the need for inducing such help in HIV vaccine strategies that aim at eliciting persistent B cell responses.

Tue May 08 00:00:00 UTC 2018

Modulation of CD4⁺ and CD8⁺ T Cell Function and Cytokine Responses in Strongyloides stercoralis Infection by Interleukin-27 (IL-27) and IL-37.

Infection and immunity

Anuradha R,Munisankar S,Bhootra Y,Dolla C,Kumaran P,Nutman TB,Babu S

17-7029 was used in Flow cytometry/Cell sorting to demonstrate that IL-27 and IL-37 limit the induction of particular T cell subsets along with cytokine responses in S. stercoralis infections, which suggest the importance of IL-27 and IL-37 in immune modulation in a chronic helminth infection.

Wed Nov 01 00:00:00 UTC 2017

PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients.

British journal of cancer

Wu X,Zhang H,Xing Q,Cui J,Li J,Li Y,Tan Y,Wang S

17-7029 was used in Immunofluorescence to evaluate the role of PD-1-PD-L1 pathway in CD8(+) T-cell functions in tumour-draining lymph nodes (TDLNs) and tumours of CRC patients.

Tue Sep 23 00:00:00 UTC 2014

Expansion of polyfunctional HIV-specific T cells upon stimulation with mRNA electroporated dendritic cells in the presence of immunomodulatory drugs.

Journal of virology

De Keersmaecker B,Allard SD,Lacor P,Schots R,Thielemans K,Aerts JL

17-7029 was used in Immunofluorescence to provide new information about the effects of IMiDs on antigen-specific T cells and suggest that these drugs increase the efficacy of immune therapies for infectious diseases and cancer.

Sat Sep 01 00:00:00 UTC 2012

Bet v 1-specific T-cell receptor/forkhead box protein 3 transgenic T cells suppress Bet v 1-specific T-cell effector function in an activation-dependent manner.

The Journal of allergy and clinical immunology

Schmetterer KG,Haiderer D,Leb-Reichl VM,Neunkirchner A,Jahn-Schmid B,Küng HJ,Schuch K,Steinberger P,Bohle B,Pickl WF

17-7029 was used in Flow cytometry/Cell sorting to demonstrate a transgenic approach to engineer customised, allergen-specific, human Treg cells, with potential implications for the treatment of allergic diseases.

Sat Jan 01 00:00:00 UTC 2011

Longevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans.

PLoS pathogens

Teirlinck AC,McCall MB,Roestenberg M,Scholzen A,Woestenenk R,de Mast Q,van der Ven AJ,Hermsen CC,Luty AJ,Sauerwein RW

17-7029 was used in Flow cytometry/Cell sorting to study cellular immunological responses to sporozoites and asexual blood-stage malaria parasites.

Thu Dec 01 00:00:00 UTC 2011

Human TCR transgenic Bet v 1-specific Th1 cells suppress the effector function of Bet v 1-specific Th2 cells.

Journal of immunology (Baltimore, Md. : 1950)

Neunkirchner A,Leb-Reichl VM,Schmetterer KG,Mutschlechner S,Kueng HJ,Haiderer D,Schuch K,Wallner M,Jahn-Schmid B,Bohle B,Pickl WF

17-7029 was used in Flow cytometry/Cell sorting to study the mechanisms underlying the cross-reactivity between the respiratory allergen, Bet v 1, and its homologous food allergens.

Sat Oct 15 00:00:00 UTC 2011

PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis.

The Journal of biological chemistry

Hrdinka M,Dráber P,Stepánek O,Ormsby T,Otáhal P,Angelisová P,Brdicka T,Paces J,Horejsí V,Drbal K

17-7029 was used in Functional assays to indicate that PRR7 is a potential regulator of signaling and apoptosis in activated T cells.

Fri Jun 03 00:00:00 UTC 2011

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

17-7029 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 UTC 2011

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

17-7029 was used in Flow cytometry/Cell sorting to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 UTC 2010

Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display.

Cancer research

Swoboda RK,Somasundaram R,Caputo L,Ochoa EM,Gimotty PA,Marincola FM,Van Belle P,Barth S,Elder D,Guerry D,Czerniecki B,Schuchter L,Vonderheide RH,Herlyn D

17-7029 was used in Flow cytometry/Cell sorting to investigate an antigen shared by melanoma and glioma cells that is recognised by T helper cells.

Sun Apr 15 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: IL-2; IL2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)

Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-2 receptor binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

Process(es)

Performance Guarantee

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IL-2 Monoclonal Antibody (MQ1-17H12), APC, eBioscience™

IL-2 Antibody (17-7029-82) in Flow

Product Details

17-7029-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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