Invitrogen

IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eBioscience™

8 Published Figures

8 References

View all (38) IL-21 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

IL-21 Antibody (14-7219-82) in ELISA

Standard curve of human IL-21 ELISA.

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Product Details

14-7219-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 8 publications 8 publications

ELISA (ELISA)

1-4 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

eBio3A3-N2 (3A3-N2)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1311258

Product Specific Information

Description: The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15.

Applications Reported: The eBio3A3-N2 antibody has been reported for use in ELISA capture of IL-21 analysis.

Applications Tested: The eBio3A3-N2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-21 (IL-21) in combination with the biotin eBio2B2-G20 (Product # 13-7218-81) antibody for detection and recombinant standard human IL-21 (Product # 39-8219-65) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 60 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria.

Cell

Rosain J,Neehus AL,Manry J,Yang R,Le Pen J,Daher W,Liu Z,Chan YH,Tahuil N,Türel Ö,Bourgey M,Ogishi M,Doisne JM,Izquierdo HM,Shirasaki T,Le Voyer T,Guérin A,Bastard P,Moncada-Vélez M,Han JE,Khan T,Rapaport F,Hong SH,Cheung A,Haake K,Mindt BC,Pérez L,Philippot Q,Lee D,Zhang P,Rinchai D,Al Ali F,Ahmad Ata MM,Rahman M,Peel JN,Heissel S,Molina H,Kendir-Demirkol Y,Bailey R,Zhao S,Bohlen J,Mancini M,Seeleuthner Y,Roelens M,Lorenzo L,Soudée C,Paz MEJ,González ML,Jeljeli M,Soulier J,Romana S,L'Honneur AS,Materna M,Martínez-Barricarte R,Pochon M,Oleaga-Quintas C,Michev A,Migaud M,Lévy R,Alyanakian MA,Rozenberg F,Croft CA,Vogt G,Emile JF,Kremer L,Ma CS,Fritz JH,Lemon SM,Spaan AN,Manel N,Abel L,MacDonald MR,Boisson-Dupuis S,Marr N,Tangye SG,Di Santo JP,Zhang Q,Zhang SY,Rice CM,Béziat V,Lachmann N,Langlais D,Casanova JL,Gros P,Bustamante J

Published figure using IL-21 monoclonal antibody (Product # 14-7219-82) in Flow Cytometry

Thu Feb 02 00:00:00 UTC 2023

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

Published figure using IL-21 monoclonal antibody (Product # 14-7219-82) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

Effects of Anti-TNFα Treatment on Mucosal Expression of IL-17A, IL-21, and IL-22 and Cytokine-Producing T Cell Subsets in Crohn's Disease.

Mediators of inflammation

Dige A,Magnusson MK,Uhrenholt C,Rasmussen TK,Kragstrup T,Öhman L,Dahlerup J,Agnholt J

Published figure using IL-21 monoclonal antibody (Product # 14-7219-82) in Flow Cytometry

Mon Oct 01 00:00:00 UTC 2018

C5aR1 regulates T follicular helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans.

JCI insight

Verghese DA,Chun N,Paz K,Fribourg M,Woodruff TM,Flynn R,Hu Y,Xiong H,Zhang W,Yi Z,Du J,Blazar BR,Heeger PS

Published figure using IL-21 monoclonal antibody (Product # 14-7219-82) in Flow Cytometry

Thu Dec 20 00:00:00 UTC 2018

Overexpression of microRNA-155 increases IL-21 mediated STAT3 signaling and IL-21 production in systemic lupus erythematosus.

Arthritis research & therapy

Rasmussen TK,Andersen T,Bak RO,Yiu G,Sørensen CM,Stengaard-Pedersen K,Mikkelsen JG,Utz PJ,Holm CK,Deleuran B

14721982 was used in flow cytometry to investigate the effect of miRNA-155 in IL-21 signaling in white cells and it role in systemic lupues erythematosus.

Tue Jun 09 00:00:00 UTC 2015

Circulating CXCR5+PD-1+ response predicts influenza vaccine antibody responses in young adults but not elderly adults.

Journal of immunology (Baltimore, Md. : 1950)

Herati RS,Reuter MA,Dolfi DV,Mansfield KD,Aung H,Badwan OZ,Kurupati RK,Kannan S,Ertl H,Schmader KE,Betts MR,Canaday DH,Wherry EJ

14-7219 was used in Flow cytometry/Cell sorting to characterise cTfh cells expressing CXCR5 and programmed death-1 (PD-1) in response to vaccination against influenza.

Wed Oct 01 00:00:00 UTC 2014

Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.

Gastroenterology

Hovhannisyan Z,Treatman J,Littman DR,Mayer L

14-7219 was used in Flow cytometry/Cell sorting to investigate how Th17 and Tregs interact under chronic inflammatory conditions.

Tue Mar 01 00:00:00 UTC 2011

Reprogramming CD19-specific T cells with IL-21 signaling can improve adoptive immunotherapy of B-lineage malignancies.

Cancer research

Singh H,Figliola MJ,Dawson MJ,Huls H,Olivares S,Switzer K,Mi T,Maiti S,Kebriaei P,Lee DA,Champlin RE,Cooper LJ

Published figure using IL-21 monoclonal antibody (Product # 14-7219-82) in Flow Cytometry

Sun May 15 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CX2; il 21; IL-21; ILN; Interleukin; Interleukin-21; interleukin-21 isoform; Interleukin21; Za11

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Gene Aliases: CVID11; IL-21; IL21; Za11

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UniProt ID: (Human) Q9HBE4

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Entrez Gene ID: (Human) 59067

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Function(s)

cytokine activity cytokine receptor binding interleukin-2 receptor binding

Process(es)

Performance Guarantee

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IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eBioscience™

IL-21 Antibody (14-7219-82) in ELISA

Product Details

14-7219-82

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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