Invitrogen

IL-23 p19 Monoclonal Antibody (G23-8), eBioscience™

IL-23 p19 Antibody in Neutralization (Neu)

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IL-23 p19 Antibody (14-7232-81) in Neu

Scientific reports 2018 - Published figure using IL-23 p19 monoclonal antibody (Product # 14-7232-81) in Neutralization

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Product Details

14-7232-81

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

ELISA (ELISA)

1-4 µg/mL

View 6 publications 6 publications

Neutralization (Neu)

View 6 publications 6 publications

Functional Assay (Functional)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

G23-8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_842742

Product Specific Information

Description: The G23-8 antibody reacts with the p19 subunit of mouse IL-23. The G23-8 antibody was generated from immunization with authentic, insect cell-expressed, recombinant mouse IL-23 heterodimer. The G23-8 antibody can specifically neutralize IL-23 bioactivity with no effect on IL-12 p70 bioactivity.

The use of a p19-specific capture antibody and a p40-specific detection antibody yields an IL-23 ELISA which is exquisitely specific for mouse IL-23. IL-12 p40 homodimer and IL-12 p70 were each run in the assay at 500 ng/mL with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was run in the IL-23 ELISA at 100 ng/mL with no cross reactivity observed; all values were at the limit of detection of the assay. For measurement of total p40 protein levels, the Mouse IL-12/23 Total p40 ELISA Ready-SET-Go! is available (Product # 88-7120-88) .

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by naive and memory T cells, as compared to IL-12.

Applications Reported: The G23-8 antibody has been reported for use as the capture antibody in mouse IL-23 ELISA, for Western blotting, and for neutralization of mouse IL-23 bioactivity.

Applications Tested: The G23-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-23 (p19p40) protein levels in combination with the biotinylated (p40-specific) C17.8 antibody (Product # 13-7123-81) for detection and recombinant mouse IL-23 (Product # 14-8231-63) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.

Important Note: TMB, rather than ABTS, should be used as a substrate to achieve this sensitivity level.

For specific neutralization of mouse IL-23 protein activity (with no effect on IL-12 p70), the functional grade purified G23-8 antibody is recommended (Product # 16-7232-81) .

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intraperitoneal administration of the anti-IL-23 antibody prevents the establishment of intestinal nematodes in mice.

Scientific reports

Gomez-Samblas M,Bernal D,Bolado-Ortiz A,Vilchez S,Bolás-Fernández F,Espino AM,Trelis M,Osuna A

14-7232 was used in Functional assays to suggest that the Th17/Th2 regulatory mechanism provoked by the administration of the anti-IL-23 antibody prevents intestinal nematode implantation in mice.

Thu May 17 00:00:00 UTC 2018

Intraperitoneal administration of the anti-IL-23 antibody prevents the establishment of intestinal nematodes in mice.

Scientific reports

Gomez-Samblas M,Bernal D,Bolado-Ortiz A,Vilchez S,Bolás-Fernández F,Espino AM,Trelis M,Osuna A

14-7232 was used in Functional assays to suggest that the Th17/Th2 regulatory mechanism provoked by the administration of the anti-IL-23 antibody prevents intestinal nematode implantation in mice.

Thu May 17 00:00:00 UTC 2018

Spirulina lipopolysaccharides inhibit tumor growth in a Toll-like receptor 4-dependent manner by altering the cytokine milieu from interleukin-17/interleukin-23 to interferon-γ.

Oncology reports

Okuyama H,Tominaga A,Fukuoka S,Taguchi T,Kusumoto Y,Ono S

14-7232 was used in an ELISA assay to examine the antitumor effects of Spirulina LPS compared to E. coli LPS in an MH134 hepatoma model.

Wed Feb 01 00:00:00 UTC 2017

Pro-inflammatory miR-223 mediates the cross-talk between the IL23 pathway and the intestinal barrier in inflammatory bowel disease.

Genome biology

Wang H,Chao K,Ng SC,Bai AH,Yu Q,Yu J,Li M,Cui Y,Chen M,Hu JF,Zhang S

14-7232 was used in Neutralization experiments to characterize a new mechanistic pathway in IBD, in which miR-223 interacts with the IL23 pathway by targeting CLDN8.

Wed Mar 30 00:00:00 UTC 2016

IL-17-producing γδ T cells and neutrophils conspire to promote breast cancer metastasis.

Nature

Coffelt SB,Kersten K,Doornebal CW,Weiden J,Vrijland K,Hau CS,Verstegen NJM,Ciampricotti M,Hawinkels LJAC,Jonkers J,de Visser KE

14-7232 was used in Neutralization experiments to show that tumours evoke a systemic inflammatory cascade to maximise their chance of metastasising in mouse models of spontaneous breast cancer metastasis.

Thu Jun 18 00:00:00 UTC 2015

A novel crosstalk between TLR4- and NOD2-mediated signaling in the regulation of intestinal inflammation.

Scientific reports

Kim H,Zhao Q,Zheng H,Li X,Zhang T,Ma X

14-7232 was used in an ELISA assay to uncover some novel activities of NOD2 by gene expression profiling revealing the global nature of the cross-regulation between TLR4- and NOD2-mediated signaling in mice.

Wed Jul 08 00:00:00 UTC 2015

The transcription factor T-bet regulates intestinal inflammation mediated by interleukin-7 receptor+ innate lymphoid cells.

Immunity

Powell N,Walker AW,Stolarczyk E,Canavan JB,Gökmen MR,Marks E,Jackson I,Hashim A,Curtis MA,Jenner RG,Howard JK,Parkhill J,MacDonald TT,Lord GM

14-7232 was used in in vivo experiments to investigate if innate lymphoid cells are responsible for driving fulminant disease in Tbx21(-/-)Rag2(-/-) ulcerative colitis mice.

Fri Oct 19 00:00:00 UTC 2012

TLR4 and NKT cell synergy in immunotherapy against visceral leishmaniasis.

PLoS pathogens

Karmakar S,Bhaumik SK,Paul J,De T

14-7232 was used in Neutralization experiments to develop a defined, immunotherapeutic strategy for the treatment of immune diseases using a mouse model of visceral leishmaniasis.

Mon Aug 20 00:00:00 UTC 2012

Overexpression of phospholipase Cε in keratinocytes upregulates cytokine expression and causes dermatitis with acanthosis and T-cell infiltration.

European journal of immunology

Takenaka N,Edamatsu H,Suzuki N,Saito H,Inoue Y,Oka M,Hu L,Kataoka T

Published figure using IL-23 p19 monoclonal antibody (Product # 14-7232-81) in Neutralization

Sat Jan 01 00:00:00 UTC 2011

IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).

PloS one

Rynda A,Maddaloni M,Ochoa-Repáraz J,Callis G,Pascual DW

14-7232 was used in an ELISA assay to demonstrate the potential for IL-28 as an alternative therapy for EAE.

Thu Jan 14 00:00:00 UTC 2010

CCR7 ligands are required for development of experimental autoimmune encephalomyelitis through generating IL-23-dependent Th17 cells.

Journal of immunology (Baltimore, Md. : 1950)

Kuwabara T,Ishikawa F,Yasuda T,Aritomi K,Nakano H,Tanaka Y,Okada Y,Lipp M,Kakiuchi T

14-7232 was used in an ELISA assay to deduce the role of CCL19 and CCL21 in inducing experimental autoimmune encephalomyelitis.

Sat Aug 15 00:00:00 UTC 2009

Endogenous antigen presenting cell-derived IL-10 inhibits T lymphocyte responses to commensal enteric bacteria.

Immunology letters

Albright CA,Sartor RB,Tonkonogy SL

14-7232 was used in an ELISA assay to study the role of interleukin-10 signalling in regulating T lymphocyte responses to commensal bacteria.

Tue Mar 24 00:00:00 UTC 2009

"Re-educating" tumor-associated macrophages by targeting NF-kappaB.

The Journal of experimental medicine

Hagemann T,Lawrence T,McNeish I,Charles KA,Kulbe H,Thompson RG,Robinson SC,Balkwill FR

14-7232-81 was used in Neutralization experiments to elucidate a novel role of NF-kappaB in cancer in maintaining the immunosuppressive phenotype of tumour-associated macrophages.

Mon Jun 09 00:00:00 UTC 2008

Inflammatory arthritis can be reined in by CpG-induced DC-NK cell cross talk.

The Journal of experimental medicine

Wu HJ,Sawaya H,Binstadt B,Brickelmaier M,Blasius A,Gorelik L,Mahmood U,Weissleder R,Carulli J,Benoist C,Mathis D

14-7232 was used in an ELISA assay to investigate the use of unmethylated CpG-oligodeoxynucleotides as antiinflammatory agents.

Mon Aug 06 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: il 23; IL 23 A; Il-12b; Il-12p40; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12B; Il12p40; il23; ILN; Interleukin; interleukin 12B; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; MGC79388; p40; RP23-388G23.1

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Gene Aliases: IL-23; Il23a; p19

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UniProt ID: (Mouse) Q9EQ14

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Entrez Gene ID: (Mouse) 83430

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Function(s)

cytokine activity protein binding interleukin-23 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-23 p19 Monoclonal Antibody (G23-8), eBioscience™

IL-23 p19 Antibody (14-7232-81) in Neu

Product Details

14-7232-81

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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