Invitrogen

IL-23 p19 Monoclonal Antibody (fc23cpg), eFluor™ 660, eBioscience™

1 Published Figure

9 References

View all (35) IL-23 p19 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

IL-23 p19 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

IL-23 p19 Antibody (50-7023-82) in Flow

Intracellular staining of unstimulated (left) or CpG ODN 2395 and monensin-treated (right) mouse dendritic cells with Anti-Mouse IL-12/23 p40 PE (Product # 12-7123-82) and Anti-Mouse IL-23 p19 eFluor® 660. Total viable cells as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) were used for analysis.

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Product Details

50-7023-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

fc23cpg

Immunogen

mouse IL-23p19 subunit

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10609336

Product Specific Information

Description: This fc23cpg monoclonal antibody reacts with the p19 subunit of mouse IL-23, a heterodimeric cytokine made up of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. IL-23 signals through a heterodimeric receptor expressed on activated T cells, macrophages, and dendritic cells. The receptor shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. Binding of IL-23 to its receptor activates Jak/STAT pathways similarly to IL-12, but the two cytokines exhibit distinct functions. Unlike IL-12, which induces T-bet and Th1 differentiation, IL-23 signaling results in RORgammat expression and supports CD4^+ T cells with the Th17 phenotype

Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as agonists of the beta-glucan receptor, Dectin-1. Our studies suggest donor, kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.

Applications Reported: This fc23cpg antibody has been reported for use by intracellular staining and flow cytometric analysis.

Applications Tested: This fc23cpg antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Enzalutamide, an Androgen Receptor Antagonist, Enhances Myeloid Cell-Mediated Immune Suppression and Tumor Progression.

Cancer immunology research

Consiglio CR,Udartseva O,Ramsey KD,Bush C,Gollnick SO

50-7023 was used in Flow cytometry/Cell sorting to investigate how AR antagonism affects myeloid cell function and metabolism in an AR-independent murine colon tumor model.

Tue Sep 01 00:00:00 UTC 2020

The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses.

Antioxidants (Basel, Switzerland)

Lee HJ,Park JS,Yoo HJ,Lee HM,Lee BC,Kim JH

50-7023 was used in Flow cytometry/Cell sorting to show methionine sulfoxide reductase B1 (MsrB1) acts in dendritic cells to regulate the phosphorylation of signal transducer and activator of transcription-6 (STAT6).

Tue Oct 20 00:00:00 UTC 2020

Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity.

Nature communications

Yang W,Yu T,Huang X,Bilotta AJ,Xu L,Lu Y,Sun J,Pan F,Zhou J,Zhang W,Yao S,Maynard CL,Singh N,Dann SM,Liu Z,Cong Y

50-7023-82 was used in Flow Cytometry to demonstrate that microbiota-derived short-chain fatty acids (SCFAs) promote IL-22 production by CD4+ T cells and ILCs through G-protein receptor 41 (GPR41) and inhibiting histone deacetylase (HDAC).

Tue Sep 08 00:00:00 UTC 2020

Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR.

Cell

Mogilenko DA,Haas JT,L'homme L,Fleury S,Quemener S,Levavasseur M,Becquart C,Wartelle J,Bogomolova A,Pineau L,Molendi-Coste O,Lancel S,Dehondt H,Gheeraert C,Melchior A,Dewas C,Nikitin A,Pic S,Rabhi N,Annicotte JS,Oyadomari S,Velasco-Hernandez T,Cammenga J,Foretz M,Viollet B,Vukovic M,Villacreces A,Kranc K,Carmeliet P,Marot G,Boulter A,Tavernier S,Berod L,Longhi MP,Paget C,Janssens S,Staumont-Sallé D,Aksoy E,Staels B,Dombrowicz D

50-7023 was used in Flow cytometry/Cell sorting to conclude that fine-tuning of innate immunity depends on optimization of metabolic demands and minimization of mtROS-induced UPR.

Thu May 16 00:00:00 UTC 2019

Induction of autophagy in Cx3cr1⁺ mononuclear cells limits IL-23/IL-22 axis-mediated intestinal fibrosis.

Mucosal immunology

Mathur R,Alam MM,Zhao XF,Liao Y,Shen J,Morgan S,Huang T,Lee H,Lee E,Huang Y,Zhu X

Published figure using IL-23 p19 monoclonal antibody (Product # 50-7023-82) in Flow Cytometry

Wed May 01 00:00:00 UTC 2019

Suppression of autophagy and HCK signaling promotes PTGS2^high FCGR3^- NK cell differentiation triggered by ectopic endometrial stromal cells.

Autophagy

Mei J,Zhou WJ,Zhu XY,Lu H,Wu K,Yang HL,Fu Q,Wei CY,Chang KK,Jin LP,Wang J,Wang YM,Li DJ,Li MQ

50-7023 was used in Flow cytometry/Cell sorting to show that the macroautophagy/autophagy level of endometrial stromal cells from endometriosis decreased under negative regulation of estrogen.

Fri Oct 11 00:00:00 UTC 2019

Central Role of IL-23 and IL-17 Producing Eosinophils as Immunomodulatory Effector Cells in Acute Pulmonary Aspergillosis and Allergic Asthma.

PLoS pathogens

Guerra ES,Lee CK,Specht CA,Yadav B,Huang H,Akalin A,Huh JR,Mueller C,Levitz SM

50702382 was used in flow cytometry to show that eosinophils act as local sources of IL-23 and IL-17 upon Aspergillus fumigatus infections

Sun Jan 01 00:00:00 UTC 2017

Interleukin (IL)-39 [IL-23p19/Epstein-Barr virus-induced 3 (Ebi3)] induces differentiation/expansion of neutrophils in lupus-prone mice.

Clinical and experimental immunology

Wang X,Liu X,Zhang Y,Wang Z,Zhu G,Han G,Chen G,Hou C,Wang T,Ma N,Shen B,Li Y,Xiao H,Wang R

50-7023 was used in Flow cytometry/Cell sorting to suggest that IL-39 induces differentiation and/or expansion of neutrophils in lupus-prone mice.

Tue Nov 01 00:00:00 UTC 2016

Mitochondrial H2O2 in Lung Antigen-Presenting Cells Blocks NF-κB Activation to Prevent Unwarranted Immune Activation.

Cell reports

Khare A,Raundhal M,Chakraborty K,Das S,Corey C,Kamga CK,Quesnelle K,St Croix C,Watkins SC,Morse C,Oriss TB,Huff R,Hannum R,Ray P,Shiva S,Ray A

50-7023 was used in Flow cytometry/Cell sorting to study how communication between PPARγ and the mitochondria maintains immune quiescence in the airways.

Tue May 24 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: il 23; IL 23 A; Il-12b; Il-12p40; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12B; Il12p40; il23; ILN; Interleukin; interleukin 12B; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; MGC79388; p40; RP23-388G23.1

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Gene Aliases: IL-23; Il23a; p19

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UniProt ID: (Mouse) Q9EQ14

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Entrez Gene ID: (Mouse) 83430

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Function(s)

cytokine activity protein binding interleukin-23 receptor binding

Process(es)

Performance Guarantee

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IL-23 p19 Monoclonal Antibody (fc23cpg), eFluor™ 660, eBioscience™

IL-23 p19 Antibody (50-7023-82) in Flow

Product Details

50-7023-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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