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IL-27 Antibody (PA5-46993) in WB
Western blot analysis of IL-27 in MT‚2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. Samples were incubated in IL-27 polyclonal antibody (Product # PA5-46993) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for the IL‚27 EBI3/IL‚27B at approximately 32 kDa and IL‚27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-46993
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-27 Arg21-Lys229 (IL-27 EBI-3 subunit), Phe29-Pro243 (IL-27 p28 subunit)
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607871
Product Specific Information
In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse IL-27, recombinant human (rh) IL-12, and rhIL-12 p40 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine receptor; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 p28 subunit; IL-27 subunit alpha; IL-27 subunit beta; IL-27-A; IL27 p28; IL27 subunit; IL27-A; IL35 subunit; ILN; Interleukin; interleukin 30; Interleukin-27 subunit alpha; Interleukin-27 subunit beta; Interleukin-30; M-IL-27 (p28); MGC71873; p28
Gene Aliases: EBI3; IL-27; IL-27A; IL-27B; IL27; IL27A; IL27B; IL27p28; IL30; p28
UniProt ID: (Human) Q14213, (Human) Q8NEV9
Entrez Gene ID: (Human) 10148, (Human) 246778
inflammatory response
response to bacterium
regulation of T cell proliferation
positive regulation of interferon-gamma biosynthetic process
innate immune response
regulation of T-helper 1 cell differentiation
regulation of defense response to virus
humoral immune response
cytokine-mediated signaling pathway
T-helper 1 type immune response
T cell proliferation
positive regulation of alpha-beta T cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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