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IL-28B Antibody (PA5-47202) in Neu
Neutralization of IL-28B in HepG2 human hepatocellular carcinoma cell line. Samples were incubated in IL-28B polyclonal antibody (Product # PA5-47202). Recombinant Mouse IL‚28B/IFN‚λ3 reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IL‚28B/IFN‚λ3 (400 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse I... View More
Product Details
PA5-47202
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse IL-28B/IFN-lambda3 Asp20-Val193
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606897
Product Specific Information
In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human IL-28A is observed. In Western blots, approximately 35% cross-reactivity (reducing conditions) and approximately 10% cross-reactivity (non-reducing conditions) with recombinant mouse IL-28A is observed. This antibody neutralizes 100% of recombinant mouse IL-28A.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-28B encodes a cytokine distantly related to type I interferons and the IL-10 family. IL-28B, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IFN-lambda 3; IFN-lambda-3; IL-28B; ILN; interferon alpha; Interferon lambda-3; Interferon lambda-4; Interleukin; interleukin 28; interleukin 28B; interleukin 28C; Interleukin-28B
Gene Aliases: IFL-1; Ifnl3; IL-28B; Il28; Il28b; INF-alpha; INF-lambda
UniProt ID: (Mouse) Q8CGK6
Entrez Gene ID: (Mouse) 338374
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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