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Leinco Technologies
IL-2R alpha (CD25) (Basiliximab) Recombinant Human Monoclonal Antibody (Hu107)
Product Details
LT300-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human,
Rhesus monkey
Host/Isotype
Human
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
Hu107
Immunogen
Human CD25
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Specificity: This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Basiliximab. Basiliximab recognizes human CD25. This product is for research use only.
Endotoxin Level: ≤ 1.0 EU/mg as determined by the LAL method. Purity: >95% by SDS-PAGE and HPLC.
Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing: To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.
This antibody has been tested in the following applications: Western Blotting, Immunohistochemistry, Immunofluorescence, Flow Cytometry, CyTOF®, Blocking.
Target Information
A recombinant chimeric (murine/human) monoclonal antibody (IgG1k) that functions as an immunosuppressive agent, specifically binding to and blocking the interleukin-2 receptor a-chain (IL-2R alpha, also known as CD25 antigen) on the surface of activated T-lymphocytes. It is a 144 kDa glycoprotein obtained from fermentation of an established mouse myeloma cell line genetically engineered to express plasmids containing the human heavy and light chain constant region genes and mouse heavy and light chain variable region genes encoding the RFT5 antibody that binds selectively to the IL-2R alpha. Basiliximab binds with high-affinity to the alpha-subunit (CD25) of the high-affinity IL-2 receptor. This inhibits IL-2 binding, which inhibits T-cell activation and prevents the body from mounting an immune response against the foreign kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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