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IL-3 beta Antibody (500-P177G-1MG) in WB
Western Blot: To detect Rat IL-3beta by Western Blot analysis IL-3 beta Polyclonal Antibody (Product # 500-P177G-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P177G-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat IL-3beta
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929655
Product Specific Information
AA Sequence of recombinant protein: MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-3beta. Anti-Rat IL-3beta-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-3beta matrix.
Sandwich ELISA: To detect Rat IL-3beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-3beta (500-P177GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-3beta.
Western Blot: To detect Rat IL-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hematopoietic growth factor; IL-3; Interleukin; interleukin-3; mast cell growth factor; MCGF; multipotential colony-stimulating factor; P-cell-stimulating factor
Entrez Gene ID: (Rat) 24495
regulation of cell growth
response to hypoxia
positive regulation of protein phosphorylation
positive regulation of myeloid leukocyte differentiation
regulation of glycolytic process
immune response
positive regulation of cell proliferation
response to hormone
regulation of gene expression
negative regulation of autophagy
cytokine-mediated signaling pathway
hemopoiesis
negative regulation of mast cell apoptotic process
myeloid cell apoptotic process
embryonic hemopoiesis
regulation of protein dephosphorylation
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of apoptotic process
positive regulation of DNA replication
positive regulation of JNK cascade
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of mast cell proliferation
extrinsic apoptotic signaling pathway in absence of ligand
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