Invitrogen

IL-4 Polyclonal Antibody

11 Published Figures

12 References

View all (106) IL-4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (11)

IL-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 14

IL-4 Antibody (PA5-25165) in IHC (P)

Immunohistochemistry analysis of IL-4 in formalin fixed and paraffin embedded tonsil. Samples were incubated with IL-4 polyclonal antibody (Product # PA5-25165) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

IL-4 Antibody in Immunohistochemistry (IHC)

IL-4 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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Product Details

PA5-25165

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

View 1 publication 1 publication

Flow Cytometry (Flow)

1:10-1:50

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 122-150 amino acids from the C-terminal region of human L4

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2542665

Product Specific Information

This antibody is predicted to react with non-human primate based on sequence homology.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A novel transgenic mouse model expressing primate-specific nuclear choline acetyltransferase: insights into potential cholinergic vulnerability.

Scientific reports

AlQot HE,Rylett RJ

PA5-25165 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to establish a novel transgenic mouse expressing 82-kDa ChAT that is valuable for studying the role of this primate-specific cholinergic enzyme in pathologies associated with cholinergic neuron vulnerability and dysfunction.

Tue Feb 21 00:00:00 UTC 2023

A novel transgenic mouse model expressing primate-specific nuclear choline acetyltransferase: insights into potential cholinergic vulnerability.

Scientific reports

AlQot HE,Rylett RJ

Tue Feb 21 00:00:00 UTC 2023

Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model.

Experimental & molecular medicine

Lee AR,Lee SY,Choi JW,Um IG,Na HS,Lee JH,Cho ML

PA5-25165 was used in Immunohistochemistry to develop the patient-derived keloid xenograft (PDKX), which is a humanized mouse model, and compared it to the traditional mouse xenograft model (transplantation of only keloid lesions).

Tue Aug 01 00:00:00 UTC 2023

Calf Thymus Polypeptide Restrains the Growth of Colorectal Tumor via Regulating the Intestinal Microbiota-Mediated Immune Function.

Frontiers in pharmacology

Li L,Zhao C,Kong F,Li YC,Wang C,Chen S,Tan HY,Liu Y,Wang D

Published figure using IL-4 polyclonal antibody (Product # PA5-25165) in Immunohistochemistry

Sat Jul 16 00:00:00 UTC 2022

Brain-derived CCR5 Contributes to Neuroprotection and Brain Repair after Experimental Stroke.

Aging and disease

Ping S,Qiu X,Kyle M,Zhao LR

Published figure using IL-4 polyclonal antibody (Product # PA5-25165) in Immunohistochemistry

Mon Feb 01 00:00:00 UTC 2021

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

PA5-25165 was used in Western Blot to demonstrate that the reduced dimeric form of Gal-1 is responsible for inducing improvements to muscle membrane repair, while the monomeric form is responsible for changes in inflammation.

Wed Nov 17 00:00:00 UTC 2021

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

Wed Nov 17 00:00:00 UTC 2021

Reparative Effects of Stem Cell Factor and Granulocyte Colony-Stimulating Factor in Aged APP/PS1 Mice.

Aging and disease

Guo X,Liu Y,Morgan D,Zhao LR

PA5-25165 was used in Immunohistochemistry-immunofluorescence to determine the therapeutic efficacy of stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) (SCF+G-CSF) in aged APPswe/PS1dE9 (APP/PS1) mice.

Tue Dec 01 00:00:00 UTC 2020

Reparative Effects of Stem Cell Factor and Granulocyte Colony-Stimulating Factor in Aged APP/PS1 Mice.

Aging and disease

Guo X,Liu Y,Morgan D,Zhao LR

Tue Dec 01 00:00:00 UTC 2020

Renal expression of cytokines and chemokines in diabetic nephropathy.

BMC nephrology

Araújo LS,Torquato BGS,da Silva CA,Dos Reis Monteiro MLG,Dos Santos Martins ALM,da Silva MV,Dos Reis MA,Machado JR

PA5-25165 was used in Immunohistochemistry to analyze the expression of cytokines and chemokines in renal biopsies from patients with DN and to correlate it with interstitial inflammation and decreased renal function.

Tue Jul 28 00:00:00 UTC 2020

Renal expression of cytokines and chemokines in diabetic nephropathy.

BMC nephrology

Araújo LS,Torquato BGS,da Silva CA,Dos Reis Monteiro MLG,Dos Santos Martins ALM,da Silva MV,Dos Reis MA,Machado JR

Tue Jul 28 00:00:00 UTC 2020

Mast Cells in Alveolar Septa of COVID-19 Patients: A Pathogenic Pathway That May Link Interstitial Edema to Immunothrombosis.

Frontiers in immunology

Motta Junior JDS,Miggiolaro AFRDS,Nagashima S,de Paula CBV,Baena CP,Scharfstein J,de Noronha L

PA5-25165 was used in Immunohistochemistry to report histopathology data obtained in post-mortem lung biopsies of COVID-19.

Mon Oct 26 00:00:00 UTC 2020

Mast Cells in Alveolar Septa of COVID-19 Patients: A Pathogenic Pathway That May Link Interstitial Edema to Immunothrombosis.

Frontiers in immunology

Motta Junior JDS,Miggiolaro AFRDS,Nagashima S,de Paula CBV,Baena CP,Scharfstein J,de Noronha L

PA5-25165 was used in Immunohistochemistry to report histopathology data obtained in post-mortem lung biopsies of COVID-19.

Mon Oct 26 00:00:00 UTC 2020

IL-4/IL-13 remodeling pathway of COVID-19 lung injury.

Scientific reports

Vaz de Paula CB,de Azevedo MLV,Nagashima S,Martins APC,Malaquias MAS,Miggiolaro AFRDS,da Silva Motta Júnior J,Avelino G,do Carmo LAP,Carstens LB,de Noronha L

PA5-25165 was used in Immunohistochemistry to conclude that understanding and managing the aggravated and ineffective immune response elicited by SARS-CoV-2 merits further clarification to improve treatments propose.

Thu Oct 29 00:00:00 UTC 2020

IL-4/IL-13 remodeling pathway of COVID-19 lung injury.

Scientific reports

Vaz de Paula CB,de Azevedo MLV,Nagashima S,Martins APC,Malaquias MAS,Miggiolaro AFRDS,da Silva Motta Júnior J,Avelino G,do Carmo LAP,Carstens LB,de Noronha L

Thu Oct 29 00:00:00 UTC 2020

STAT6 deficiency ameliorates Graves' disease severity by suppressing thyroid epithelial cell hyperplasia.

Cell death & disease

Jiang X,Zha B,Liu X,Liu R,Liu J,Huang E,Qian T,Liu J,Wang Z,Zhang D,Wang L,Chu Y

PA525165 was used in immunohistochemistry - paraffin section to explore the contribution of STAT6 phosphorylation to Graves' disease and study its role in thyroid epithelial cells

Thu Dec 01 00:00:00 UTC 2016

STAT6 deficiency ameliorates Graves' disease severity by suppressing thyroid epithelial cell hyperplasia.

Cell death & disease

Jiang X,Zha B,Liu X,Liu R,Liu J,Huang E,Qian T,Liu J,Wang Z,Zhang D,Wang L,Chu Y

PA525165 was used in immunohistochemistry - paraffin section to explore the contribution of STAT6 phosphorylation to Graves' disease and study its role in thyroid epithelial cells

Thu Dec 01 00:00:00 UTC 2016

Sequential delivery of immunomodulatory cytokines to facilitate the M1-to-M2 transition of macrophages and enhance vascularization of bone scaffolds.

Biomaterials

Spiller KL,Nassiri S,Witherel CE,Anfang RR,Ng J,Nakazawa KR,Yu T,Vunjak-Novakovic G

Published figure using IL-4 polyclonal antibody (Product # PA5-25165) in Immunofluorescence

Thu Jan 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112, (Mouse) P07750

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Entrez Gene ID: (Human) 3565, (Mouse) 16189

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Function(s)

cytokine activity interleukin-4 receptor binding protein binding growth factor activity cytokine receptor binding

Process(es)

Performance Guarantee

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IL-4 Polyclonal Antibody

IL-4 Antibody (PA5-25165) in IHC (P)

Product Details

PA5-25165

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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