Invitrogen

IL-4 Monoclonal Antibody (B-S4), eBioscience™

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

BMS129

Applications
Tested Dilution
Publications

Functional Assay (Functional)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

B-S4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596493

Product Specific Information

Description: IL-4 (B-S4) is a mouse monoclonal antibody raised against IL-4 of humanorigin.

BMS129FI is suitable for intracellular staining of IL-4.

Human IL-4 exists in molecular weight forms between 15,000 and 19,000 daltons, representing variable glycosylation. The gene for human IL-4 is found on the long arm of chromsome 5 in close association with genes for IL-13, IL-5 GM-CSF and IL-3.IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be up-regulated by IL-2 and IL-4.

IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

Applications Tested: Functional Studies (Blocking).

Purity: >95%.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)
cytokine activity interleukin-4 receptor binding protein binding growth factor activity
Process(es)
microglial cell activation innate immune response in mucosa positive regulation of defense response to virus by host T-helper 1 cell lineage commitment negative regulation of acute inflammatory response negative regulation of chronic inflammatory response chemotaxis immune response cellular defense response female pregnancy response to nutrient cholesterol metabolic process regulation of proton transport negative regulation of epithelial cell migration response to organic cyclic compound B cell differentiation positive regulation of B cell proliferation B cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-13 production response to cytokine T-helper 2 cell cytokine production type 2 immune response positive regulation of T cell proliferation positive regulation of activated T cell proliferation regulation of phosphorylation response to drug positive regulation of tyrosine phosphorylation of Stat5 protein defense response to protozoan myeloid dendritic cell differentiation negative regulation of macrophage activation negative regulation of apoptotic process positive regulation of mast cell degranulation negative regulation of nitric oxide biosynthetic process T-helper 2 cell differentiation positive regulation of chemokine biosynthetic process connective tissue growth factor biosynthetic process regulation of isotype switching positive regulation of MHC class II biosynthetic process response to ethanol positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes regulation of immune response positive regulation of sequence-specific DNA binding transcription factor activity retina development in camera-type eye negative regulation of white fat cell proliferation cellular response to mercury ion positive regulation of mononuclear cell migration dendritic cell differentiation extrinsic apoptotic signaling pathway in absence of ligand positive regulation of myoblast fusion negative regulation of complement-dependent cytotoxicity negative regulation of T-helper 17 cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of eosinophil chemotaxis negative regulation of extrinsic apoptotic signaling pathway
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