Invitrogen

IL-6 Polyclonal Antibody

IL-6 Antibody in Immunocytochemistry (ICC/IF)

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IL-6 Antibody (P620) in ICC/IF

Immunofluorescence analysis of IL-6 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IL-6 Polyclonal Antibody (Product # P620) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More

IL-6 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

IL-6 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using IL-6 Polyclonal Antibody (Product # P620), shows upregulation in the expression of IL-6 upon treatment of HUVEC cells with LPS and Brefeldin A, HaCaT cells with Poly(I:C) and PTI, and Poly(dA:dT) and PTI. {TM}

Product Details

P620

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

1:50-1:200

ELISA (ELISA)

1:50-1:5,000

View 6 publications 6 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human IL-6.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_223481

Product Specific Information

P620 detects IL-6 in WB, Flow and ELISA applications with human samples.

The P620 immunogen is recombinant human IL-6.

P620 detects IL-6 which has a predicted molecular weight of approximately 22 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Protective Effect of Fucoxanthin on Zearalenone-Induced Hepatic Damage through Nrf2 Mediated by PI3K/AKT Signaling.

Marine drugs

Ben Ammar R,Zahra HA,Abu Zahra AM,Alfwuaires M,Abdulaziz Alamer S,Metwally AM,Althnaian TA,Al-Ramadan SY

Published figure using IL-6 polyclonal antibody (Product # P620) in Western Blot

Mon Jul 03 00:00:00 UTC 2023

Polypropylene nanoplastic exposure leads to lung inflammation through p38-mediated NF-κB pathway due to mitochondrial damage.

Particle and fibre toxicology

Woo JH,Seo HJ,Lee JY,Lee I,Jeon K,Kim B,Lee K

Published figure using IL-6 polyclonal antibody (Product # P620) in Western Blot

Tue Jan 10 00:00:00 UTC 2023

Oxidative stress and inflammation distinctly drive molecular mechanisms of diastolic dysfunction and remodeling in female and male heart failure with preserved ejection fraction rats.

Frontiers in cardiovascular medicine

Zhazykbayeva S,Hassoun R,Herwig M,Budde H,Kovács Á,Mannherz HG,El-Battrawy I,Tóth A,Schmidt WE,Mügge A,Hamdani N

P620 was used in Western Blotting to suggest that oxidative stress and inflammation distinctly drive diastolic dysfunction and remodeling in female and male rats with HFpEF and that the sex-dependent mechanisms contribute to HF pathology.

Sat Jul 01 00:00:00 UTC 2023

Dihydromyricetin ameliorates osteogenic differentiation of human aortic valve interstitial cells by targeting c-KIT/interleukin-6 signaling pathway.

Frontiers in pharmacology

Zhang S,Fan L,Wang Y,Xu J,Shen Q,Xie J,Zeng Z,Zhou T

P620 was used in Immunocytochemistry to show that DHM markedly ameliorated osteogenic induction medium (OM)-induced osteogenic differentiation of hVICs, as evidenced by downregulation of ALP and Runx2 expression and decreased calcium deposition.

Fri Aug 26 00:00:00 UTC 2022

Dihydromyricetin ameliorates osteogenic differentiation of human aortic valve interstitial cells by targeting c-KIT/interleukin-6 signaling pathway.

Frontiers in pharmacology

Zhang S,Fan L,Wang Y,Xu J,Shen Q,Xie J,Zeng Z,Zhou T

Fri Aug 26 00:00:00 UTC 2022

Design of Targeted Flurbiprofen Biomimetic Nanoparticles for Management of Arthritis: In Vitro and In Vivo Appraisal.

Pharmaceutics

Mohamed HI,El-Kamel AH,Hammad GO,Heikal LA

Published figure using IL-6 polyclonal antibody (Product # P620) in Western Blot

Fri Jan 07 00:00:00 UTC 2022

CD38-NADase is a new major contributor to Duchenne muscular dystrophic phenotype.

EMBO molecular medicine

de Zélicourt A,Fayssoil A,Dakouane-Giudicelli M,De Jesus I,Karoui A,Zarrouki F,Lefebvre F,Mansart A,Launay JM,Piquereau J,Tarragó MG,Bonay M,Forand A,Moog S,Piétri-Rouxel F,Brisebard E,Chini CCS,Kashyap S,Fogarty MJ,Sieck GC,Mericskay M,Chini EN,Gomez AM,Cancela JM,de la Porte S

P620 was used in Immunohistochemistry (PFA) to develop for DMD patients.

Mon May 09 00:00:00 UTC 2022

PIAS1 Alleviates Hepatic Ischemia-Reperfusion Injury in Mice through a Mechanism Involving NFATc1 SUMOylation.

Disease markers

Luo J,Li J,Li T,Zhang Z,Chen G,Li Q,Qi H,Si Z

P620 was used in Western Blotting to show that the NFATc1/HDAC1/IRF-1/p38 MAPK signaling axis is highlighted as a promising therapeutic target for potentiating hepatoprotective effects of PIAS1 against HIRI.

Tue Sep 13 00:00:00 UTC 2022

Downregulation of APRIN expression increases cancer cell proliferation via an interleukin-6/STAT3/cyclin D axis.

Oncology letters

Sohn MS,Kang M,Kang SM,Bae S

Published figure using IL-6 polyclonal antibody (Product # P620) in Neutralization

Fri Jan 01 00:00:00 UTC 2021

Luteolin Improves Cyclophosphamide-Induced Cystitis through TXNIP/NLRP3 and NF-κB Pathways.

Evidence-based complementary and alternative medicine : eCAM

Zhang H,Zhao J,Lu Q,Sun B,Liu X,Yang C,Li S,Li L,Yi S,Yang Z,Xu J

Published figure using IL-6 polyclonal antibody (Product # P620) in Western Blot

Tue Nov 23 00:00:00 UTC 2021

β2-Adrenergic agonists attenuate organic dust-induced lung inflammation.

American journal of physiology. Lung cellular and molecular physiology

Romberger DJ,Heires AJ,Nordgren TM,Poole JA,Toews ML,West WW,Wyatt TA

Published figure using IL-6 polyclonal antibody (Product # P620) in ELISA

Fri Jul 01 00:00:00 UTC 2016

Redox control of the senescence regulator interleukin-1α and the secretory phenotype.

The Journal of biological chemistry

McCarthy DA,Clark RR,Bartling TR,Trebak M,Melendez JA

Published figure using IL-6 polyclonal antibody (Product # P620) in Neutralization

Fri Nov 08 00:00:00 UTC 2013

Redox control of the senescence regulator interleukin-1α and the secretory phenotype.

The Journal of biological chemistry

McCarthy DA,Clark RR,Bartling TR,Trebak M,Melendez JA

Published figure using IL-6 polyclonal antibody (Product # P620) in Neutralization

Fri Nov 08 00:00:00 UTC 2013

Quantification of the neutralization of cytokine biological activity by antibody: the ten-fold reduction bioassay of interleukin-6 as growth factor.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Grossberg SE,Casey M,Grossberg LD

Published figure using IL-6 polyclonal antibody (Product # P620) in Neutralization

Sat Aug 01 00:00:00 UTC 2009

Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.

Blood

Burke SM,Issekutz TB,Mohan K,Lee PW,Shmulevitz M,Marshall JS

P620 was used in ELISA to study the role of human mast cells in the recruitment of natural killer cells

Sun Jun 15 00:00:00 UTC 2008

Combined immunogene therapy of IL-6 and IL-15 enhances anti-tumor activity through augmented NK cytotoxicity.

Cancer letters

Lin CY,Chuang TF,Liao KW,Huang YJ,Pai CC,Chu RM

P620 was used in immunocytochemistry to study the ability of combined IL-6 and IL-15 immunogene therapy to enhance natural killer cell cytotoxicity and anti-tumor activity

Thu Dec 18 00:00:00 UTC 2008

Interleukin-6 fused to an anti-idiotype antibody in a vaccine increases the specific humoral immune response against CA125+ (MUC-16) ovarian cancer.

Cancer research

Reinartz S,Hombach A,Köhler S,Schlebusch H,Wallwiener D,Abken H,Wagner U

P620 was used in blocking or activating experiment to study the capacity of IL-6 fused to an anti-idiotype antibody in a vaccine to increase the specific humoral immune response against CA125+ ovarian cancer

Sun Jun 15 00:00:00 UTC 2003

Changes in macrophages in spleen and lymph nodes during acute African swine fever: expression of cytokines.

Veterinary immunology and immunopathology

Salguero FJ,Ruiz-Villamor E,Bautista MJ,Sánchez-Cordón PJ,Carrasco L,Gómez-Villamandos JC

P620 was used in immunohistochemistry to study the role of cytokine expression by macrophages in spleen and lymph nodes in the pathogenesis of African swine fever

Fri Nov 01 00:00:00 UTC 2002

Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis.

The Journal of clinical investigation

Rasmussen SJ,Eckmann L,Quayle AJ,Shen L,Zhang YX,Anderson DJ,Fierer J,Stephens RS,Kagnoff MF

P620 was used in ELISA to investigate the important roles of epithelial cells in inflammatory response to chlamydia

Wed Jan 01 00:00:00 UTC 1997

Interleukin-6 stimulates neutrophil production of platelet-activating factor.

Journal of leukocyte biology

Biffl WL,Moore EE,Moore FA,Barnett CC,Silliman CC,Peterson VM

P620 was used in blocking/activating experiment to study the effect of interleukin 6 on the platelet-activating factor production by neutrophils

Mon Apr 01 00:00:00 UTC 1996

Entamoeba histolytica trophozoites induce an inflammatory cytokine response by cultured human cells through the paracrine action of cytolytically released interleukin-1 alpha.

The Journal of clinical investigation

Eckmann L,Reed SL,Smith JR,Kagnoff MF

P620 was used in ELISA to study the effect of Entamoeba histolytica trophozoites on cytokine expression

Fri Sep 01 00:00:00 UTC 1995

A distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion.

The Journal of clinical investigation

Jung HC,Eckmann L,Yang SK,Panja A,Fierer J,Morzycka-Wroblewska E,Kagnoff MF

P620 was used in ELISA to investigate the expression of proinflammatory cytokines in human colon epithelial cells

Sun Jan 01 00:00:00 UTC 1995

Localization of cytokines in cholesteatoma tissue.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery

Marenda SA,Aufdemorte TB

P620 was used in immunohistochemistry to investigate the distribution of various cytokines in cholesteatoma tissue

Wed Mar 01 00:00:00 UTC 1995

Serum IL-4, IL-10 and IL-6 levels in inflammatory arthritis.

Rheumatology international

Cicuttini FM,Byron KA,Maher D,Wootton AM,Muirden KD,Hamilton JA

P620 was used in ELISA to investigate the interleukin expression and secretion in patients with inflalmnatory arthritis

Mon May 22 00:00:00 UTC 1995

Soluble IgA and IgG aggregates are catabolized by cultured rat mesangial cells and induce production of TNF-alpha and IL-6, and proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Gómez-Guerrero C,López-Armada MJ,González E,Egido J

P620 was used in blocking/activating experiment to study the mechanism for the mesangial deposits of IgA immune complexes in IgA nephropathy

Thu Dec 01 00:00:00 UTC 1994

Differential expression and ligand-induced modulation of the human interleukin-6 receptor on interleukin-6-responsive cells.

The Journal of biological chemistry

Schwabe M,Zhao J,Kung HF

P620 was used in immunoprecipitation to investigate the differential expression and regulation of interleukin 6 receptor

Fri Mar 11 00:00:00 UTC 1994

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231, (Mouse) P08505

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Entrez Gene ID: (Human) 3569, (Mouse) 16193

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Function(s)

cytokine activity interleukin-6 receptor binding protein binding growth factor activity receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-6 Polyclonal Antibody

IL-6 Antibody (P620) in ICC/IF

Product Details

P620

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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