Invitrogen

IL-6 Monoclonal Antibody (MQ2-13A5), APC, eBioscience™

6 Published Figures

10 References

View all (211) IL-6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

FIGURE: 1 / 7

IL-6 Antibody (17-7069-42) in Flow

Intracellular staining of normal human peripheral blood cells unstimulated (left) or stimulated with LPS and monensin (right) with Anti-Human CD14 PerCP-Cy5-5 (Product # 45-0149-42) and Anti-Human IL-6 APC. Cells in the monocyte gate were used for analysis.

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Product Details

17-7069-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ2-13A5

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10853009

Product Specific Information

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: This MQ2-13A5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MQ2-13A5 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

BCG revaccination in adults enhances pro-inflammatory markers of trained immunity along with anti-inflammatory pathways.

iScience

Ahmed A,Tripathi H,van Meijgaarden KE,Kumar NC,Adiga V,Rakshit S,Parthiban C,Eveline J S,D'Souza G,Dias M,Ottenhoff THM,Netea MG,Joosten SA,Vyakarnam A

Published figure using IL-6 monoclonal antibody (Product # 17-7069-42) in Flow Cytometry

Fri Oct 20 00:00:00 UTC 2023

Peripheral Blood Mononuclear Cells from Patients with Type 1 Diabetes and Diabetic Retinopathy Produce Higher Levels of IL-17A, IL-10 and IL-6 and Lower Levels of IFN-γ-A Pilot Study.

Cells

Obasanmi G,Lois N,Armstrong D,Hombrebueno JMR,Lynch A,Chen M,Xu H

17-7069-42 was used in Flow cytometry/Cell sorting to investigate alterations in inflammatory cytokines in peripheral blood mononuclear cells (PBMCs) in 41 people with type 1 diabetes (T1D), sub-grouped into mild non-proliferative DR (mNPDR; n = 13) and active and inactive (each n = 14) PDR.

Tue Jan 31 00:00:00 UTC 2023

Interleukin 6 regulates the expression of programmed cell death ligand 1 in thyroid cancer.

Cancer science

Zhang GQ,Jiao Q,Shen CT,Song HJ,Zhang HZ,Qiu ZL,Luo QY

Published figure using IL-6 monoclonal antibody (Product # 17-7069-42) in Flow Cytometry

Mon Mar 01 00:00:00 UTC 2021

Exposure to wild-type AAV drives distinct capsid immunity profiles in humans.

The Journal of clinical investigation

Kuranda K,Jean-Alphonse P,Leborgne C,Hardet R,Collaud F,Marmier S,Costa Verdera H,Ronzitti G,Veron P,Mingozzi F

17-7069 was used in Flow cytometry/Cell sorting to show that prior exposure to adeno-associated virus(AAV) drives distinct capsid immunity profiles in healthy subjects.

Mon Dec 03 00:00:00 UTC 2018

Exposure to wild-type AAV drives distinct capsid immunity profiles in humans.

The Journal of clinical investigation

Kuranda K,Jean-Alphonse P,Leborgne C,Hardet R,Collaud F,Marmier S,Costa Verdera H,Ronzitti G,Veron P,Mingozzi F

17-7069 was used in Flow cytometry/Cell sorting to show that prior exposure to adeno-associated virus(AAV) drives distinct capsid immunity profiles in healthy subjects.

Mon Dec 03 00:00:00 UTC 2018

"Inflamm-aging" influences immune cell survival factors in human bone marrow.

European journal of immunology

Pangrazzi L,Meryk A,Naismith E,Koziel R,Lair J,Krismer M,Trieb K,Grubeck-Loebenstein B

17-7069 was used in Flow cytometry/Cell sorting to show that the expression of molecules involved in the maintenance of immunological memory in the human bone marrow changes with age.

Wed Mar 01 00:00:00 UTC 2017

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Zhou Q,Yu X,Demirkaya E,Deuitch N,Stone D,Tsai WL,Kuehn HS,Wang H,Yang D,Park YH,Ombrello AK,Blake M,Romeo T,Remmers EF,Chae JJ,Mullikin JC,Güzel F,Milner JD,Boehm M,Rosenzweig SD,Gadina M,Welch SB,Özen S,Topaloglu R,Abinun M,Kastner DL,Aksentijevich I

Published figure using IL-6 monoclonal antibody (Product # 17-7069-42) in Flow Cytometry

Tue Sep 06 00:00:00 UTC 2016

Regulatory T cells that co-express RORγt and FOXP3 are pro-inflammatory and immunosuppressive and expand in human pancreatic cancer.

Oncoimmunology

Chellappa S,Hugenschmidt H,Hagness M,Line PD,Labori KJ,Wiedswang G,Taskén K,Aandahl EM

17-7069 was used in Flow cytometry/Cell sorting to investigate the phenotype and functional properties of regulatory T cells in patients with pancreatic ductal adenocarcinoma.

Fri Apr 01 00:00:00 UTC 2016

Evidence for innate immune system activation in HIV type 1-infected elite controllers.

The Journal of infectious diseases

Krishnan S,Wilson EM,Sheikh V,Rupert A,Mendoza D,Yang J,Lempicki R,Migueles SA,Sereti I

17-7069 was used in Flow cytometry/Cell sorting to study how elite controllers maintain control of plasma HIV viremia and have evidence of an activated innate immune response.

Sat Mar 01 00:00:00 UTC 2014

Monocyte subsets in schistosomiasis patients with periportal fibrosis.

Mediators of inflammation

Fernandes JS,Araujo MI,Lopes DM,de Souza Rda P,Carvalho EM,Cardoso LS

17-7069 was used in Flow cytometry/Cell sorting to suggest that the three different monocyte populations participate in the immunopathogenesis of periportal fibrosis.

Mon Dec 15 00:00:00 UTC 2014

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

Published figure using IL-6 monoclonal antibody (Product # 17-7069-42) in Flow Cytometry

Mon Sep 08 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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Function(s)

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

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IL-6 Monoclonal Antibody (MQ2-13A5), APC, eBioscience™

IL-6 Antibody (17-7069-42) in Flow

Product Details

17-7069-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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