Invitrogen

IL-8 (CXCL8) Monoclonal Antibody (3IL8-H10)

IL-8 (CXCL8) Antibody in Immunocytochemistry (ICC/IF)

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IL-8 (CXCL8) Antibody (M801) in ICC/IF

Immunofluorescence analysis of IL-8 was performed using 70% confluent log phase U-87 MG cells treated 1 uM thapsigargin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IL-8 (3IL8-H10) Mouse Monoclonal Antibody (Product # M801) at 2µg/mL in 0.1%... View More

IL-8 (CXCL8) Antibody in Radioimmune assays (RIA)

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Product Details

M801

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 27 publications 27 publications

Radioimmune Assays (RIA)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3IL8-H10

Immunogen

Recombinant human IL-8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_223583

Product Specific Information

M801 targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.

The M801 immunogen is recombinant human IL-8.

M801 detects IL-8 which has a predicted molecular weight of approximately 9 kDa.

The M801 IL8 antibody (clone 3IL8-H10) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M802B (biotinylated conjugate of clone I8-S2). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M801 (clone 3IL8-H10) and biotinylated antibody M802B (clone I8-S2) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An Evaluation of the Cytotoxic and Genotoxic Effects of the Marine Toxin C17-SAMT in Human TK6 and HepaRG Cell Lines.

International journal of molecular sciences

Marzougui Z,Le Hegarat L,Hogeveen K,Huet S,Kharrat R,Marrouchi R,Fessard V

M801 was used in Enzyme-linked immunosorbent assay to highlight the potential toxicity of C17-SAMT and the need for further research to better understand its effects.

Tue Apr 25 00:00:00 UTC 2023

Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells.

Journal of periodontology

Kantrong N,Chang AM,Bamashmous S,Hajjar AM,Bucala RJ,Darveau RP

M801 was used in Enzyme-linked immunosorbent assay to demonstrate that MIF is a significant contributor to the maintenance of healthy oral homeostasis.

Thu Dec 01 00:00:00 UTC 2022

Sensing plasma membrane pore formation induces chemokine production in survivors of regulated necrosis.

Developmental cell

Wang W,Prokopec JS,Zhang Y,Sukhoplyasova M,Shinglot H,Wang MT,Linkermann A,Stewart-Ornstein J,Gong YN

M801 was used in Immunohistochemistry (Frozen) to find that cells which undergo limited plasma membrane damage from the pore-forming actions of MLKL, GSDMD, perforin, or detergents experience local activation of PKCs through Ca2+ influx at the damage sites.

Mon Jan 24 00:00:00 UTC 2022

Chronic effects of two rutile TiO₂ nanomaterials in human intestinal and hepatic cell lines.

Particle and fibre toxicology

Jalili P,Krause BC,Lanceleur R,Burel A,Jungnickel H,Lampen A,Laux P,Luch A,Fessard V,Hogeveen K

M801 was used in Enzyme-linked immunosorbent assay to indicate that although no clear effects on cytotoxicity were observed following repeated exposure of differentiated Caco-2 and HepaRG cells to TiO2 NMs, subtle effects on membrane composition could induce potential adverse effects in the long-term.

Tue May 17 00:00:00 UTC 2022

Impact of Progerin Expression on Adipogenesis in Hutchinson-Gilford Progeria Skin-Derived Precursor Cells.

Cells

Najdi F,Krüger P,Djabali K

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in Immunocytochemistry

Fri Jun 25 00:00:00 UTC 2021

Neuropilin-1 is up-regulated by cancer-associated fibroblast-secreted IL-8 and associated with cell proliferation of gallbladder cancer.

Journal of cellular and molecular medicine

Chen C,Zhang R,Ma L,Li Q,Zhao YL,Zhang GJ,Zhang D,Li WZ,Cao S,Wang L,Geng ZM

M801 was used in an ELISA assay to demonstrate how Neuropilin-1 causes gallbladder cancer cell proliferation through upregulation by cancer-associated fibroblast-secreted IL-8.

Sun Nov 01 00:00:00 UTC 2020

Neuropilin-1 is up-regulated by cancer-associated fibroblast-secreted IL-8 and associated with cell proliferation of gallbladder cancer.

Journal of cellular and molecular medicine

Chen C,Zhang R,Ma L,Li Q,Zhao YL,Zhang GJ,Zhang D,Li WZ,Cao S,Wang L,Geng ZM

M801 was used in an ELISA assay to demonstrate how Neuropilin-1 causes gallbladder cancer cell proliferation through upregulation by cancer-associated fibroblast-secreted IL-8.

Sun Nov 01 00:00:00 UTC 2020

Fipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal epithelial cells.

Rhinology

Kwak S,Choi YS,Na HG,Bae CH,Song SY,Kim YD

M801 was used in Enzyme-linked immunosorbent assay to evaluate the effect of fipronil on pro-inflammatory cytokine and mucus production and signalling pathways in human primary nasal.

Sat Feb 01 00:00:00 UTC 2020

Combined effects of okadaic acid and pectenotoxin-2, 13-desmethylspirolide C or yessotoxin in human intestinal Caco-2 cells.

Chemosphere

Alarcan J,Barbé S,Kopp B,Hessel-Pras S,Braeuning A,Lampen A,Le Hégarat L,Fessard V

M801 was used in ELISA to examine the effects of three binary mixtures, OA/PTX-2, OA/SPX-1 and OA/YTX on human intestinal Caco-2 cells.

Thu Aug 01 00:00:00 UTC 2019

The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping.

Journal of immunology research

Nikolić N,Kienzl P,Tajpara P,Vierhapper M,Matiasek J,Elbe-Bürger A

M801 was used in an ELISA assay to investigate the influence of octenidine on epidermal Langerhans cells and cytokine secretion pattern of skin cells during wound healing in a model without disruption of the normal skin structure.

Thu Jul 18 00:00:00 UTC 2019

Extravascular gelation shrinkage-derived internal stress enables tumor starvation therapy with suppressed metastasis and recurrence.

Nature communications

Zhang K,Fang Y,He Y,Yin H,Guan X,Pu Y,Zhou B,Yue W,Ren W,Du D,Li H,Liu C,Sun L,Chen Y,Xu H

M801 was used in an ELISA assay to establish an extravascular gelation shrinkage-derived internal stress strategy for squeezing and narrowing blood vessels, occluding blood & nutrition supply, reducing vascular density, inducing hypoxia and apoptosis and eventually realizing starvation therapy of malignancies.

Tue Nov 26 00:00:00 UTC 2019

Resolvin D2 decreases TLR4 expression to mediate resolution in human monocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Croasdell A,Sime PJ,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in ELISA

Thu Sep 01 00:00:00 UTC 2016

Indole derivatives inhibit hepatitis C virus replication through induction of pro-inflammatory cytokines.

Acta virologica

Lee S,Jin G,Kim D,Son S,Lee K,Lee C

M801 was used in ELISA, Immunocytochemistry, Western Blot, Flow Cytometry to find a novel mode of action of indole derivatives in inhibiting HCV replication, particularly the induction of pro-inflammatory cytokines.

Sun Mar 01 00:00:00 UTC 2015

The human salivary proteome is radiation responsive.

Radiation research

Moore HD,Ivey RG,Voytovich UJ,Lin C,Stirewalt DL,Pogosova-Agadjanyan EL,Paulovich AG

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in Radioimmune Assays

Thu May 01 00:00:00 UTC 2014

Microparticles engineered to highly express peroxisome proliferator-activated receptor-γ decreased inflammatory mediator production and increased adhesion of recipient monocytes.

PloS one

Sahler J,Woeller CF,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in ELISA

Mon Jul 13 00:00:00 UTC 2015

Attenuation of inflammatory mediator production by the NF-κB member RelB is mediated by microRNA-146a in lung fibroblasts.

American journal of physiology. Lung cellular and molecular physiology

McMillan DH,Woeller CF,Thatcher TH,Spinelli SL,Maggirwar SB,Sime PJ,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in ELISA

Sat Jun 01 00:00:00 UTC 2013

Transforming growth factor-β impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line.

British journal of pharmacology

Salem S,Harris T,Mok JS,Li MY,Keenan CR,Schuliga MJ,Stewart AG

M801 was used in ELISA to investigate the effect of transforming growth factor-beta on glucocorticoid activity in human A549 cells

Wed Aug 01 00:00:00 UTC 2012

Ocular fibroblast diversity: implications for inflammation and ocular wound healing.

Investigative ophthalmology & visual science

Xi X,McMillan DH,Lehmann GM,Sime PJ,Libby RT,Huxlin KR,Feldon SE,Phipps RP

M801 was used in ELISA to study the significance of ocular fibroblast diversity for inflammation and ocular wound healing

Fri Jul 01 00:00:00 UTC 2011

Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?

Arthritis and rheumatism

Page TH,Brown A,Timms EM,Foxwell BM,Ray KP

M801 was used in ELISA to investigate the role of p38 kinase in rheumatoid arthritis synovial membranes

Mon Nov 01 00:00:00 UTC 2010

Intermittent haemodiafiltration in refractory congestive heart failure: BNP and balance of inflammatory cytokines.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Libetta C,Sepe V,Zucchi M,Pisacco P,Cosmai L,Meloni F,Campana C,Rampino T,Monti C,Tavazzi L,Dal Canton A

M801 was used in ELISA to assess the interleukin-8 level in patients with decompensated congestive heart failure

Sun Jul 01 00:00:00 UTC 2007

IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.

Journal of immunology (Baltimore, Md. : 1950)

Saffar AS,Alphonse MP,Shan L,Hayglass KT,Simons FE,Gounni AS

M801 was used in ELISA to study the role of mitochondrial pathway in neutrophil survival

Thu Feb 15 00:00:00 UTC 2007

Does the type of surgery effect systemic response following cardiopulmonary bypass?

Journal of cardiac surgery

Takayama H,Soltow LO,Chandler WL,Vocelka CR,Aldea GS

M801 was used in Enzyme-linked immunosorbent assay to conclude that valve surgery induced more prominent systemic response than CABG.

Mon Sep 17 00:00:00 UTC 2007

Expression of a Porphyromonas gingivalis lipid A palmitylacyltransferase in Escherichia coli yields a chimeric lipid A with altered ability to stimulate interleukin-8 secretion.

Cellular microbiology

Bainbridge BW,Coats SR,Pham TT,Reife RA,Darveau RP

M801 was used in an ELISA assay to study the cloning, expression and characterisation of a Porphyromonas gingivalis htrB homolog.

Sun Jan 01 00:00:00 UTC 2006

Protein microarray platform for the multiplex analysis of biomarkers in human sera.

Journal of immunological methods

Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F

M801 was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera

Fri Oct 20 00:00:00 UTC 2006

Oxidized ATP (oATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms.

British journal of pharmacology

Beigi RD,Kertesy SB,Aquilina G,Dubyak GR

M801 was used in ELISA to study the role of oxidized ATP in proinflammatory signaling

Wed Oct 01 00:00:00 UTC 2003

Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease.

Cell biology and toxicology

Urbanowska T,Mangialaio S,Hartmann C,Legay F

M801 was used in ELISA to develop protein microarray technology to monitor biomarkers of rheumatoid arthritis disease

Sun Jun 01 00:00:00 UTC 2003

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Transplantation proceedings

Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M

M801 was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients

Sun Jun 01 00:00:00 UTC 2003

Limitation of thrombin generation, platelet activation, and inflammation by elimination of cardiotomy suction in patients undergoing coronary artery bypass grafting treated with heparin-bonded circuits.

The Journal of thoracic and cardiovascular surgery

Aldea GS,Soltow LO,Chandler WL,Triggs CM,Vocelka CR,Crockett GI,Shin YT,Curtis WE,Verrier ED

M801 was used in Enzyme-linked immunosorbent assay to study efficacy of heparin-bonded circuits to blunt inflammation, platelet dysfunction, and thrombin generation in response to cardiopulmonary bypass have varied.

Mon Apr 01 00:00:00 UTC 2002

Regulation of interleukin 8 expression in human malignant melanoma cells.

Cancer research

Singh RK,Varney ML

M801 was used in ELISA to investigate the regulation of interleukin 8 expression in melanoma cells

Wed Apr 01 00:00:00 UTC 1998

Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis.

The Journal of clinical investigation

Rasmussen SJ,Eckmann L,Quayle AJ,Shen L,Zhang YX,Anderson DJ,Fierer J,Stephens RS,Kagnoff MF

M801 was used in ELISA to investigate the important roles of epithelial cells in inflammatory response to chlamydia

Wed Jan 01 00:00:00 UTC 1997

Antineutrophil cytoplasmic antibodies induce monocyte IL-8 release. Role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors.

The Journal of clinical investigation

Ralston DR,Marsh CB,Lowe MP,Wewers MD

M801 was used in ELISA to investigate the role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors in monocyte interleukin 8 release

Mon Sep 15 00:00:00 UTC 1997

Monocyte Fc gamma receptor cross-linking induces IL-8 production.

Journal of immunology (Baltimore, Md. : 1950)

Marsh CB,Gadek JE,Kindt GC,Moore SA,Wewers MD

M801 was used in ELISA to investigate the influence of monocyte Fc gamma receptor cross-linking on the expression of interleukin 8

Fri Sep 15 00:00:00 UTC 1995

Rapamycin treatment depresses intragraft expression of KC/MIP-2, granzyme B, and IFN-gamma in rat recipients of cardiac allografts.

Journal of immunology (Baltimore, Md. : 1950)

Wieder KJ,Hancock WW,Schmidbauer G,Corpier CL,Wieder I,Kobzik L,Strom TB,Kupiec-Weglinski JW

M801 was used in immunohistochemistry to investigate the effects of rapamycin on KC/MIP-2, granzyme B, and interferon gamma expression on cardiac allografts

Thu Jul 15 00:00:00 UTC 1993

Bioinformatics

Protein Aliases: alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

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Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

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UniProt ID: (Human) P10145

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Entrez Gene ID: (Human) 3576

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Function(s)

interleukin-8 receptor binding protein binding chemokine activity

Process(es)

angiogenesis response to molecule of bacterial origin movement of cell or subcellular component chemotaxis inflammatory response immune response cell cycle arrest signal transduction G-protein coupled receptor signaling pathway negative regulation of cell proliferation calcium-mediated signaling regulation of cell adhesion neutrophil chemotaxis receptor internalization response to endoplasmic reticulum stress intracellular signal transduction PERK-mediated unfolded protein response neutrophil activation cellular response to fibroblast growth factor stimulus regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis embryonic digestive tract development induction of positive chemotaxis chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of neutrophil chemotaxis

Performance Guarantee

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IL-8 (CXCL8) Monoclonal Antibody (3IL8-H10)

IL-8 (CXCL8) Antibody (M801) in ICC/IF

Product Details

M801

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Radioimmune Assays (RIA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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