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IL2RG Antibody (PA5-47409) in Neu
Neutralization of IL2RG in MO7e human megakaryocytic leukemic cell line. Samples were incubated in IL2RG polyclonal antibody (Product # PA5-47409). Recombinant Human IL‚2 stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‚2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Common γ Chain/IL‚2 Rγ Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 20-60 µg/mL.
Product Details
PA5-47409
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Sf 21-derived recombinant human Common gamma Chain/IL-2R gamma Leu23-Asn254
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608968
Product Specific Information
In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL-2 R gamma is observed, less than 10% cross-reactivity with recombinant human (rh) IL-2 R beta is observed, and less than 2% cross-reactivity with rhIL-2 R alpha is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <1.0 EU per 1 µg of the antibody by the LAL method.
Target Information
IL2RG is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD132; CD132 antigen; common cytokine receptor gamma chain; Cytokine receptor common subunit gamma; gammaC; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL2R gamma; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; p64
Gene Aliases: CD132; CIDX; IL-2RG; IL2RG; IMD4; P64; SCIDX; SCIDX1
UniProt ID: (Human) P31785
Entrez Gene ID: (Human) 3561
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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