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IL31RA Antibody (PA5-120973) in WB
Western blot analysis of IL31RA in U-87MG cells. Samples were incubated with IL31RA Polyclonal antibody (Product # PA5-120973) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-120973
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-321 of human IL31RA (NP_0012845011)
Conjugate
Form
Liquid
Concentration
0.16 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914545
Product Specific Information
Positive test controls include: Mouse liver, Mouse heart, Mouse brain, Mouse pancreas. The target is usually found in the following locations: Cell membrane, Single-pass type I membrane protein.
Immunogen sequence: VKPVLGIKRM IQIEWIKPEL APVSSDLKYT LRFRTVNSTS WMEVNFAKNR KDKNQTYNLT GLQPFTEYVI ALRCAVKESK FWSDWSQEKM GMTEEEAPCG LELWRVLKPA EADGRRPVRL LWKKARGAPV LEKTLGYNIW YYPESNTNLT ETMNTTNQQL ELHLGGESFW VSMISYNSLG KSPVATLRIP AIQEKSFQCI EVMQACVAED QLVVKWQSSA LDVNTWMIEW FPDVDSEPTT LSWESVSQAT NWTIQQDKLK PFWCYNISVY PMLHDKVGEP YSIQAYAKEG VPSEGPETKV EN
Target Information
IL-31 is a T cell cytokine that is preferentially produced by T helper type 2 cells. IL-31 signals through a heterodimeric receptor composed of the IL-31 receptor (IL-31R) and the oncostatin M receptor (OSM). This receptor complex recruits JAK1, JAK2, Stat1, Stat3 and Stat5 signaling pathways, as well as the PI3 kinase/AKT cascade. SHP-2 and Shc adapter molecules are also recruited and contribute to an increased activation of the MAP kinase pathway in response to IL-31. Overexpression of IL-31 in mice results in pruritus and skin dermatitis resembling human atopic dermatitis (AD). Comparisons between skin from patients with AD and healthy skin showed IL-31R expression at higher levels on epidermal keratinocytes in AD samples. Infiltrating cells, more numerous in skin from patients with AD compared with that of healthy individuals, expressed IL-31 mRNA. IL-31 may participate in the cause of itch sensation and promote scratching behavior in NC/Nga mice with atopic dermatitis, and may represent a novel target for antipruritic drug development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: class I cytokine receptor; CRL 3; CRL3Glmr; cytokine receptor NR10; Cytokine receptor-like 3; GLM R; GLM-R; GLM-RMGC125346; GP130 like receptor; Gp130-like monocyte receptor; Gp130-like receptor; HGLM R; hGLM-R; HGLMR; IL 31RA; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL-31R-alpha; IL-31RA; IL-31RAGPLCRL; IL31R subunit alpha; interleukin 31RA; Interleukin-31 receptor subunit alpha; MGC125346; mGLM-R; Novel cytokine receptor 10; NR10; PLCA 2; soluble type I cytokine receptor CRL3; ZcytoR17
Gene Aliases: CRL; CRL3; GLM-R; GLMR; GPL; hGLM-R; IL-31RA; IL31RA; NR10; PLCA2; PRO21384; UNQ6368/PRO21073/PRO21384
UniProt ID: (Human) Q8NI17, (Mouse) Q8K5B1
Entrez Gene ID: (Human) 133396, (Mouse) 218624
immune system process
positive regulation of cell proliferation
cytokine-mediated signaling pathway
MAPK cascade
glandular epithelial cell differentiation
acute inflammatory response to antigenic stimulus
defense response
transmembrane receptor protein tyrosine kinase signaling pathway
JAK-STAT cascade
monocyte differentiation
macrophage differentiation
T-helper 2 cell cytokine production
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
homeostatic process
negative regulation of macrophage activation
negative regulation of apoptotic process
positive regulation of transcription, DNA-templated
defense response to other organism
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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