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ILF3 Antibody (PA5-115420) in ICC/IF
Immunocytochemistry analysis of ILF3 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115420) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115420
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ILF3(Accession Q12906), corresponding to amino acid residues Q319-P369.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900056
Product Specific Information
Antibody detects endogenous levels of total ILF3.
Target Information
UBA6 (UBE1L2, MOP-4)is a novel E1 enzyme specific for ubiquitin and belongs to the ubiquitin-activating E1 family. It is a part of the cascade to attach ubiquitin (Ub) covalently to substrate proteins. UBA6 activates ubiquitin by first adenylating its carboxy-terminal glycine residue with ATP and then linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Its isoforms influence spermatogenesis and male fertility. UBA6 is highly expressed in human monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Double-stranded RNA-binding protein 76; double-stranded RNA-binding protein, 76 kD; DRBP76; dsRNA binding protein NFAR-2/MPP4; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; Interleukin enhancer-binding factor 3; M phase phosphoprotein 4; M-phase phosphoprotein 4; MPP4; NF-AT-90; NFAR; nuclear factor 90; Nuclear factor associated with dsRNA; Nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; TCP80; Translational control protein 80
Gene Aliases: CBTF; DRBF; DRBP76; ILF3; MBII-26; MMP4; MPHOSPH4; MPP4; NF-AT-90; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR-1; NFAR2; TCP110; TCP80
UniProt ID: (Human) Q12906, (Mouse) Q9Z1X4, (Rat) Q9JIL3
Entrez Gene ID: (Human) 3609, (Mouse) 16201, (Rat) 84472
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
protein methylation
negative regulation of translation
negative regulation of viral genome replication
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
defense response to virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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